The invention provides a multivalent pneumococcal conjugate vaccine. The vaccine consists of a liquid injection and lyophilized powder; the liquid injection is the conjugate that contains 15 pneumococcal serotypes 1, 2, 3, 4, 5, 6A, 6B, 7F, 9V, 12F, 14, 18C, 19A, 19F and 23F and is prepared by taking diphtheria toxoid as carrier protein; and the lyophilized powder is the conjugate that contains 8pneumococcal serotypes 8, 10A, 11A, 15B, 22F, 24F, 33F and 35B and is prepared by taking tetanus toxoid as the carrier protein. Through the combined using after the lyophilized powder is dissolved inthe liquid injection, the vaccine can be used for preventing the infection caused by the pneumococcus including the serotypes.