Preparation method of type B haemophilus influenzae capsular polysaccharide and combined vaccine

Abstract

The invention discloses a preparation method of a type B haemophilus influenzae capsular polysaccharide. The preparation method comprises the steps of fermentation culture, sterilization and precipitation, polysaccharide extraction and purification, polysaccharide derivatization and polysaccharide protein conjugate preparation. The invention further discloses a multivalent combined vaccine which is prepared through the steps that an acellular pertussis stock solution, refined diphtheria toxoid and refined tetanus toxoid are added into aluminum hydroxide to be adsorbed and then added into a type B haemophilus influenzae capsular polysaccharide stock solution, and the mixed solution is diluted with normal saline. According to the preparation method, the prior art is optimized, and the high-purity low-impurity-content type B haemophilus influenzae capsular polysaccharide can be obtained; meanwhile, the type B haemophilus influenzae capsular polysaccharide is combined with multiple component vaccines to form the multivalent vaccine, the multivalent vaccine can be immune to multiple diseases simultaneously, the inoculating times are decreased, the medical risk is reduced, and the better immune effect can be obtained through one-time inoculating.

Description

technical field [0001] The invention relates, in particular, to a preparation method of Haemophilus influenzae type B capsular polysaccharide and a combined vaccine. Background technique [0002] Haemophilus influenzae is a common pathogenic bacterium, which is only pathogenic to humans, and can be divided into encapsulated strains and non-encapsulated strains. Capsular strains are divided into six serotypes (a-f) according to the specific antigens of capsular polysaccharides, among which type b antigens are the most virulent and are the main pathogenic bacteria of children with sepsis, meningitis and pneumonia. The Hib capsule is composed of lipopolysaccharide and capsular polysaccharide, the main component is ribose phosphate alcohol phosphate (polyriboylribitolphosphate, PRP), its structure includes a ribose, a ribitol and a phosphate base. [0003] Hib is transmitted by droplets and mainly infects children under 5 years old. Neonates transmit serum bactericidal antibod...

AI Technical Summary

Problems solved by technology

[0008] The DTaP vaccine to prevent diphtheria, tetanus and pertussis has been widely used in infant immunization in China, and the Hib conjugate vaccine to prevent infant meningitis and pneumonia has been successfully developed and included in the local expanded immunization program. The immunization procedures of the two vaccines are the same. At present, DTaP-Hib combined vaccines have been commercially available abroad, but DTaP-Hib combined vaccines have not been successfully developed in China.