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Preparation method for absorbed acellular diphtheria, tetanus and pertussis combined vaccine

A cell-free DTP and combined vaccine technology, used in the prevention of pertussis, tetanus, and diphtheria, can solve the problems of insufficient vaccine quantity and vaccine supply gap.

Inactive Publication Date: 2012-07-04
CHENGDU KANGHUA BIOLOGICAL PROD
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  • Abstract
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AI Technical Summary

Problems solved by technology

Many developed regions in China have begun to use acellular DPT vaccine on a large scale, but the amount of vaccine produced is far from meeting the demand, resulting in a large vaccine supply gap

Method used

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  • Preparation method for absorbed acellular diphtheria, tetanus and pertussis combined vaccine

Examples

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Effect test

example 1

[0091] Example 1 Preparation of acellular pertussis stock solution, refined diphtheria and tetanus toxoid

[0092] 1.1. Preparation of acellular pertussis stock solution

[0093] 1.1.1 Pertussis phase I strain 58003 (CS) inoculation and BG blood medium large tube culture for 3 to 4 generations

[0094] The culture time is selected to be 38 to 44 hours, and when the pH value is controlled to be about 8.5, the bacteria concentration and hemagglutination activity of B. pertussis are higher.

[0095] 1.1.2 Inoculation of fermenter culture

[0096] Add the prepared SSM medium into a 10L fermenter, and the culture concentration of inoculated bacteria is 3.0×10 8 / ml, the culture temperature is 37°C, the ventilation volume is 40-60L / min, and the pressure is 0.1kg / cm 2 , the rotational speed of the stirrer was set at 350rpm. The pH, concentration and hemagglutination of the fermentation broth were measured when the culture reached 26, and samples were taken every 3 hours to measur...

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Abstract

The invention relates to a method using stain cultivation toxoid to prepare diphtheria, tetanus and pertussis vaccine, which uses modes of ammonium sulfate salting out, density gradient centrifugation and glutaraldehyde detoxification to prepare acellular pertussis raw liquid containing effective antigen of PT and FHA. acellular diphtheria, tetanus and pertussis combined vaccine for preventing diphtheria, tetanus and pertussis can be finally prepared by preparing the pertussis raw liquid and refining and purifying to obtain mixture of diphtheria toxoid and tetanus toxoid.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine, and relates to a toxoid prepared from pertussis, diphtheria and tetanus strains to prepare a diphtheria vaccine for preventing three diseases of pertussis, diphtheria and tetanus. Background technique: [0002] Whooping cough is a common, highly contagious respiratory infection. Whooping cough is called Tianxiaocho, Luchanhou, and plague cough in traditional Chinese medicine. Our country has a treatment method for this disease as early as the eleventh century. Monlton mentioned whooping cough in 1840, and Sydenham described it in detail in 1876, calling it a severe coughing disease. Pertussis bacillus was found in 1900 by Bordet and Gengon in sputum smears of children. Due to the high nutritional requirements for the initial isolation of the bacteria, it was not until 1906 that they used the blood-glycerol-potato medium to obtain pure species. Bacillus pertussis can cause acute respirator...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61P31/04A61K39/05A61K39/08A61K39/10
Inventor 侯文礼廖常茹冯晓赵志鹏钟泽荣
Owner CHENGDU KANGHUA BIOLOGICAL PROD
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