Live vaccine (CC gene stock) of cytokine adjuvant in animal immunopotentiator for treating chicken new castle disease
A chicken Newcastle disease and cytokine technology, applied in the direction of antiviral agents, virus antigen components, antibody medical components, etc., can solve the problems of low immune function of animal populations, reduced animal production performance, immune system disorders, etc., and achieve good clinical application effect, Guaranteeing human health and enhancing humoral immunity
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[0011] The specific plan is as follows:
[0012] 1. Calf thymus - bleaching - extraction of connective tissue and fat - mincing - homogenization - repeated freezing and thawing - drying - extraction - centrifugation - ultrafiltration - thymosin ultrafiltrate - intermediate product inspection.
[0013] 2. Pig spleen—rinsing—removal of connective tissue and fat—mashing—homogenization—repeated freezing and thawing—centrifugation—ultrafiltration—transfer factor ultrafiltrate—intermediate product inspection.
[0014] 3. Chicken Newcastle disease attenuated strain (CC strain) - chicken embryo fibroblasts - nutrient solution for maintenance solution - adjust PH value - observe cell lesions - freeze-thaw - attenuated cell culture - intermediate product inspection.
[0015] 4. Prepare 10mg / ml thymosin ultrafiltrate—prepare 5mg / ml transfer factor ultrafiltrate—prepare every 0.1ml containing 10 8.5 TCID 50 Attenuated cell cultures - mixed in a ratio of 0.5:1:0.1.
[0016] 5. Freeze-dr...
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