40 results about "Drugs toxicity" patented technology

The invention discloses a fed-batch type two-layer cell co-culture chip which comprises a chip slot, a porous membrane, a sleeve layer and a top cover, wherein the chip slot comprises a lower cell culture area and three uniformly distributed flow choking barriers; an upper culture liquid inlet, an upper reagent inlet, a lower culture liquid inlet, a lower reagent inlet, an upper outlet and a lower outlet are formed in the outer part of the chip slot; an upper cell culture area and three uniformly distributed U-shaped flow choking barriers are arranged on the front side of the sleeve layer; a sleeve layer gap and three fixed barriers are arranged on the back side of the sleeve layer. The fed-batch type two-layer cell co-culture chip is capable of co-culturing two layers of cells, particularly due to design of the porous membrane, cell non-contact type information communication of upper and lower layers of culture systems can be achieved, the growth situation of cells and drug toxicity or pharmacology activity can be detected at real time on line, and the fed-batch type two-layer cell co-culture chip has the characteristics of simplicity, portability, economy, efficiency and accuracy, and can be popularized to mutual action relationship of multiple organs and tissues, medicine screening and toxicity evaluation.

The invention provides nucleic acids, peptides, and antibodies for use in applications including diagnosis and therapy. The peptides target lung cancer and were identified by phage display. Targeting phage PC5-2 and synthetic peptide SP5-2 were both able to recognize human pulmonary tumor specimens from lung cancer patients. In SCID mice bearing NSCLC xenografts, the targeting phage was able to target tumor masses specifically. When the peptide was coupled to liposomes containing the anti-cancer drugs vinorelbine or doxorubicin, the efficacy of these drugs against human lung cancer xenografts was improved, the survival rate increased, and the drug toxicity was reduced.

A microfabricated multi-tissue system for in vitro drug toxicity testing having a plurality of layers, each of which is formed of decellularized tissue extracellular matrix (ECM) including parenchymal cells and non-parenchymal cells attached thereto. Also disclosed is a method for producing a decellularized ECM paste, and methods for producing an ECM construct and a porous 3-D scaffold from the decellularized ECM paste.

The invention relates to an application method of a combined reagent in copper and sulphur separation of ore beneficiation of covellite predominantly copper sulfide ore. The invention comprises the following steps: size mixing with lime after grinding of raw ores to enable the pH value of raw ore pulps to be 7 to 12, utilizing loatation an O - isopropyl - N - sulfur ethyl carbamate and sodium butyl xanthogenate to obtain an accumulated opper and sulphur mixing concentrate; grinding the accumulated copper and sulphur mixing concentrate to reach the required grinding fineness in the copper and sulphur separation, and directly putting the combined reagent with the lime and an copper collector into the copper and sulphur separation work to activate easy oxidized copper blue minerals and restrain iron pyrites to the effective separation of the copper and the sulphur. Utilizing the application method of the combined reagent in copper and sulphur separation of ore beneficiation of covellite predominantly copper sulfide ore can achieve the effective separation effect of the copper and sulphur, and sodium sulphide and sodium humate can effectively restrain pyrite; in a same separation effect, the using amount of the combined reagent only amounts to 40% to 50% of the lime and other inhabitors; and drug toxicity is small and is environmentally friendly.

The invention discloses a triazole compound. The triazole compound has excellent water solubility. The triazole compound is obtained by introduction of a novel functional group to high efficiency and wide spectrum antifungal compound voriconazole and fluconazole drugs found in the existing clinical application and novel drug development so that the triazole compound greatly improves voriconazole and fluconazole water solubility and reduces drug toxicity.

The invention discloses a movable processing method and a device. The movable processing method and device are aimed at dispersity and complexity generated by oily sludge, and drug toxicity caused by refining process, and conduct innocent treatment upon self heating value of the oily sludge. A combination processing method of thermo-chemistry refining and pyrolysis burning is adopted to conduct intelligent combination aimed at geographical features and mechanism of production of the oily sludge. A total craft process includes: oily sludge earlier stage processing -> pyrolysis burning -> desorption -> filtering -> cyclic utilization -> discharging, wherein each module not only has an independent function, but also has a combination function, and can achieve functions such as pyrolysis burning, decomposition absorption, crude oil recovery, sludge elutriation, sludge drying and sludge solidification. The oily sludge after processed does not contain petroleum and other injurious ingredient and accords to environment-friendly discharging requirement, and the system can continuously operate for 24 hours, conducts crude oil recovery for sludge with high oil, and achieves the aims of reduction, harmlessness and reclamation of treatment and disposal of hazardous wastes.

The invention discloses a doxorubicin hydrochloride self-assembled polymer nanoparticle which is prepared from doxorubicin hydrochloride and an amphipathic high-molecular polymer PLGA (Polylactic-co-Glycolic Acid), wherein the PLGA is formed by two monomers, namely lactic acid (LA) and glycolic acid (GA). The preparation method comprises the following steps: 1) dissolving a drug doxorubicin hydrochloride into a polar organic solvent A so as to obtain a doxorubicin hydrochloride solution; 2) dissolving the PLGA into a polar organic solvent B so as to obtain a PLGA solution; 3) uniformly mixing the doxorubicin hydrochloride solution with the PLGA solution so as to obtain an eutectic solution, dripping the eutectic solution into an aqueous phase under the stirring condition, continuously stirring, and volatilizing the organic solvent; and 4) centrifuging the completely volatilized solution at a high speed by using a centrifugal machine, and collecting the precipitate, thereby obtaining the doxorubicin hydrochloride self-assembled polymer nanoparticle. The prepared product can achieve the effects of reducing the drug toxicity, reducing the administration frequency, improving the drug stability, delaying in-vivo release time of the drug, reducing adverse reactions of the drug, improving the bioavailability and efficacy of the drug and achieving excellent treatment effects.

The invention discloses a coronary artery dilating catheter carrying ligustrazine nanoparticles. The coronary artery dilating catheter is characterized by comprising a sharp end, a guide wire channel, a far end part of the catheter, a sacculus of which the outer surface carries the ligustrazine nanoparticles, a near end part of the catheter, a side hole and a replenishing device, wherein the sharp end is connected to the guide wire channel and is communicated with the side hole; one end of the sacculus is fixed to the guide wire channel; the other end of the sacculus is connected to the far end part of the catheter; the side hole is located in the far end part of the catheter outside the sacculus; the far end part of the catheter is connected to the near end part of the catheter; the near end part of the catheter is connected to the replenishing device; the guide wire channel, the far end part of the catheter and the sacculus are not communicated. During dilation, ligustrazine nanoparticle medicines on the coronary artery dilating catheter can directly contact the inner coronary artery plaque, and the resistance is reduced; meanwhile, compared with dilating catheters of other medicines, the coronary artery dilating catheter carrying the ligustrazine nanoparticles has the advantages that the drug toxicity of the ligustrazine nanoparticle medicines is smaller, and the price is low.

The present invention discloses a high-value utilization method of lignin in black liquor. The pH value of the black liquor is regulated step by step by using dilute acid such as sulfuric acid and thelike, so that lignin is graded and precipitated; and the obtained lignin is purified by using an ethanol / water mixed solution, so that the obtained lignin has high purity and uniform molecular weightdistribution. Through the step-by-step precipitation, the lignin can be effectively classified based on the molecular size, and further development and utilization of lignin samples are facilitated.Hydrophobic modification treatment is carried out on the lignin sample through an acetylation reaction, and then dissolution performance of lignin in an organic solvent-water mixed system can be changed, so that the lignin can be used as a raw material to prepare novel materials such as drug release controlling agents, nano adsorption materials and the like, and thus the application range of lignin is expanded. The method provided by the invention has the advantages of low cost investment, low drug toxicity, less required equipment, simple operation method and easy realization of large-scale production and application.

The invention discloses a quick-acting drug abstinence de-addiction pure Chinese medicinal preparation and a preparation method thereof. The quick-acting drug abstinence de-addiction pure Chinese medicinal preparation consists of two parts, namely a drug abstinence, toxin expulsion and detoxication Chinese medicinal preparation A and a drug abstinence, body resistance strengthening and mind tranquilizing Chinese medicinal preparation B, and belongs to the field of traditional Chinese medicines. The quick-acting drug abstinence de-addiction pure Chinese medicinal preparation is preferably prepared by refining raw materials such as cordyceps sinensis, American ginseng, astragalus, Chinese angelica, Japanese milkwort herb, liquoric root, baical skullcap root, golden thread, yanhusuo, medicinal evodia fruit, Chinese date, honeysuckle flower, spina date seed, thinleaf milkwort root-bark and the like. The quick-acting drug abstinence de-addiction pure Chinese medicinal preparation has the effects of supporting healthy energy, expelling drug toxicity, abstaining drugs, promoting flow of Qi and blood circulation, dispersing cold to ease pain, calming the heart, tranquilizing the mind and the like. The preparation has reasonable compatibility of the medicines, has a simple preparation process, is convenient to take, has quick response and has no adverse side effect or addiction, the patient is quickly recovered and does not need injection, transfusion or care, and the preparation is one of the most effective medicines for drug abstinence at present.

The invention discloses lotus rhizome plantation high-efficiency fertilizer. The lotus rhizome plantation high-efficiency fertilizer comprises, by weight, 10-20 parts of plant straws, 15-20 parts of turf, 1-5 parts of urea, 4-8 parts of bean cake, 8-12 parts of Chinese medical stone, 9-13 parts of boric acid, 5-7 parts of potassium carbonate, 10-15 parts of itaconic acid, 3-5 parts of trace elements and 1-3 parts of a growth conditioning agent. The lotus rhizome plantation high-efficiency fertilizer greatly improves a lotus rhizome yield. The lotus rhizome treated by the lotus rhizome plantation high-efficiency fertilizer has good quality and a long fresh-keeping period, has disease prevention, bacteriostasis, stress resistance and no drug toxicity, is rich in boron and potassium, satisfies crop growth and development requirements and improves economic benefits.

The invention relates to a quality control method for a drug microtox assay system, wherein the quality control method comprises the following steps: (1) reference substance solution preparation: taking ZnSO4.7H2O, dissolving, preparing ZnSO4.7H2O solutions with the concentrations respectively of 5.0 mg.L<-1>, 20.0 mg.L<-1>, 40.0 mg.L<-1>, 80.0 mg.L<-1>, 100.0 mg.L<-1> and the like, and then respectively mixing with a 20% sodium chloride solution with the volume ratio of 17:3, to obtain a series of solutions as tested solutions of points of a standard curve; (2) test bacterial liquid preparation: taking vibrio fischeri, restoring, to obtain a test bacterial liquid, and determining the luminous intensity; and (3) detection: taking the reference substance solutions of the step (1), adding the test bacterial liquid, respectively determining the luminous intensities, drawing the standard curve, and carrying out accompanying quality control of a sample solution in the process of detection. The quality control method used for the drug microtox assay system and established by the method has the advantages of high sensitivity, high precision, high accuracy and good stability, can be used for detection of drug toxicity, and has good application prospect.

The invention discloses a derivative of pyridine and (4,3-d) pyrimidine-1(2H)-base phenyl acetamide and an application thereof. The derivative has good pharmaceutical activity, can greatly prolong the half-life periods of drugs, prolongs the retention time of drugs in the human body, and meanwhile increases the drug concentration in the blood. Therefore, a better curative effect is achieved. As the half-life periods of drugs are greatly prolonged, the drugs can keep activity concentration for a longer time in the blood. Under the cure condition with dose limiting, the curative effect can be kept and the recommended dosage of drugs can be reduced at the same time, therefore the bad metabolism of drugs is eliminated, the drug toxicity is reduced, and the toxic and side effects in the drug using process are small.

The invention discloses a feed additive capable of enhancing immunity of livestock and poultry, and relates to the technical field of feed additives. The feed additive consists of bighead atractylodesrhizomes, radix astragali, ginkgo seeds, fresh ginger, licorice roots, bean dregs, radish seeds, hedyotis diffusa, caulis polygoni multiflori and zinc oxide. The additive is prepared through fermentation of purely natural plants, so that the autologous nonspecific immunity of animals can be aroused, and the effects of treating diseases when diseases occur and preventing diseases when no diseasesoccur can be achieved; and the fermented feed additive facilitates absorption by organisms, besides, the speed of curative effects is increased, after absorption for 30 minutes, the feed additive canreach blood, the drug effects are improved, the absorption speed of the feed additive is increased by 5-10 times than that of conventional traditional Chinese medicine powder, drug toxicity is degraded, and the feed additive is really free from toxic and side effects. The product namely the feed additive has the functions of invigorating qi and consolidation of superficies, raising yang, clearingheat, removing toxicity and the like, the immunity of animals can be greatly enhanced, the occurrence of common diseases of livestock and poultry can be prevented, and the healthy growth of the livestock and the poultry is promoted. The feed additive disclosed by the invention is simple in production technology, convenient to use, low in price and environmentally-friendly, and is an ideal feed additive for animals, and the raw materials are easy to obtain.

The invention discloses a Chinese herbal medicine feed additive for Dongchuan pigs and a Dongchuan pig feeding method. The Chinese herbal medicine feed additive is prepared from the following components in percentage by weight: 14-16% of medicated leaven, 8-10% of malt, 8-10% of garlic, 8-12% of astragalus membranaceus, 8-10% of codonopsis pilosula, 10-12% of polygonum multiflorum, 14-16% of angelica sinensis and 15-20% of liquorice. The Chinese herbal medicine feed additive is simple to prepare and good in effect. By using Chinese herbal medicine additives in the feed, the residuals of additives such as antibiotics and chemical synthetic drugs in pork can be decreased, the carcass character and the pork quality can be remarkably improved, the carcass meat factor can be improved, the drug residue can be decreased, the chemical drug toxicity can be decreased and guarantee can be provided to the food safety of people.

The invention discloses a heat-clearing detoxifying agent and a preparation method thereof. A specific extraction method is employed to extract effective components from mung beans and licorice in the raw materials, then natural plant ingredients are added to conduct pretreatment on part of the raw materials, the pretreated raw materials can strengthen the effects of the Chinese medicinal extracts and natural plant ingredients, the prepared heat-clearing detoxifying agent not only has the efficacy of removing mould and expelling toxin, harmonizing drug properties, easing drug toxicity and virulence, and protecting animal health, but also has certain therapeutic action on bronchitis.

The invention discloses a double-drug-loading self-healing hydrogel system and a preparation method thereof. The preparation method comprises the steps of adopting oxidized pullulan, epsilon-polylysine and low molecular weight polyethyleneimine as raw materials, adding two anti-cancer drugs such as cis-platinum and doxorubicin hydrochloride while preparing a hydrogel, and generating dynamic imine linkage through Schiff base reaction and crosslinking to prepare the double-drug-loadingself-healinghydrogel system. The preparation method provided by the invention is simple to operate, and short in preparation period; the prepared hydrogel system has biocompatibility, syringeability and self-healing property, and is loaded with two drugs at the same time, so that combined chemotherapy can be carried out on malignant tumors through parenteral administration around the tumor, the drugs play tosynergistic effect, a curative effect is enhanced, and the drug toxicity and the drug resistance are reduced.

The invention discloses nutritive honey syrup for alleviating drug toxicity and a preparation method of the nutritive honey syrup. The efficacies of honey for removing toxicity and nursing the intestine and the stomach are fully exerted, and through auxiliary materials, ingredients and a preparation method, the purposes of alleviating the drug toxicity and reducing the toxic and side effects of drugs can be realized after people eat larger dosage of finished products for three treatment courses. The nutritive honey syrup for alleviating the drug toxicity disclosed by the invention comprises raw materials, auxiliary materials and ingredients, wherein the raw materials comprise the following component in parts by weight: 35-40 parts of honey; the auxiliary materials comprise the following components in parts by weight: 15-20 parts of anthocyanidin fruits and 10-15 parts of anthocyanidin leaves; and the ingredients comprise the following components in parts by weight: 5-10 parts of red jujubes, 5-10 parts of haws, 5-10 parts of cassia twigs, 5-10 parts of Chinese wolfberry fruits, 5-10 parts of lemons, 5-10 parts of licorice roots and 5 parts of fresh ginger.

A novel method useful in drug screening. The method is useful for testing effects of substances on the mitochondria, notably toxic or beneficial effects of drug substances or candidate drug substances. The method is based on measurement in live human mitochondria ex vivo, but in a setting as near the in vivo situation as possible. The method is also useful for testing substances impact on the mitochondrial respiration. The method can be used to i) screening and selection of early or late stage drug candidates in cells derived from blood from healthy individuals or in so-called buffy coat, which is a concentrated solution of platelets and white blood cells, ii) testing a patient's sensitivity to a known mitochondrial toxicant, iii) analysing mitochondrial drug toxicity in clinical trials, and / or iv) analysing beneficial effects of drugs intended to improve mitochondrial function

The invention provides a combined medicine. The combined medicine consists of artemisinin which serves as a first active ingredient, hydroxychloroquine sulfate which serves as a second active ingredient and optional pharmaceutically acceptable adjuvants. The combined medicine can be used for treating malaria more effectively, and the combined medicine can relieve drug toxicity and delay the generation of drug resistance.

The invention is a kind of bupleurum root ext extracted from the southern bupleurum root and used to cure the hyperpiasia diseases and its method of extraction. The active components of the bupleurumroot ext at least includes the heterocyclic compound, with the Z or E position of the carbon 2(5), centered around the gamma- butyrolactone and the new bupleurum root lactone, isobupleurum root lactone, bupleurum root lactone similar and its ramification contained in the heterocyclic compound. From the results of the experiments of the drug toxicity, characteristics of the cell, animal experimentsand histologic section, we can know that the ext of the outhern bupleurum root can restrain the cancer cells of the liver cancer, lung cancer, oophoroma, large intestine cancer or swart brain glia cell tumour and specially kill the tomour cells with drug resistance to the taxales medicines after the chemotherapy. It can be a new resource of the drug screening of the tumor inhibitor.

The present invention relates to a high-efficiency traditional Chinese medicine anticancer product and its preparation method, use method and application. The effective anti-cancer ingredients contained in the present invention do not contain or less contain ineffective ingredients that do not have anti-cancer functions and have potential drug toxicity; Combined with the spray drying process, it is applied to the production of traditional Chinese medicine anticancer products, and combined with the optimized use method, the solubility, the absorption rate of the human body, the content of active ingredients and the medicinal efficacy of the product are improved, and the toxicity of the product to the human body is reduced. , has a wide range of applications.

The invention discloses an efficient and lasting termite control preparation. The preparation is prepared from a kusnezoff monkshood root extract, a radix aconite extract, a pinellia ternata extract, a chaulmoogratree seed extract, a juvenile hormone pesticide, imidacloprid, fipronil, rosin powder, a stabilizing agent and an antioxidant through mixing. A preparation method of the efficient and lasting termite control preparation comprises steps as follows: the kusnezoff monkshood root extract, the radix aconite extract, the pinellia ternata extract and the chaulmoogratree seed extract are stirred uniformly, and a first mixture is obtained; then the juvenile hormone pesticide, imidacloprid and fipronil are mixed and stirred uniformly, and a second mixture is obtained; finally, after the first mixture and the second mixture are mixed, the rosin powder, the stabilizing agent and the antioxidant are added and further stirred uniformly, and a finished product is obtained. Compared with the prior art, the efficient and lasting termite control preparation and the preparation method have the advantages that the efficient and lasting termite control preparation is prepared from Chinese and western medicines through reasonable compounding, integrates characteristics of the Chinese and western medicines, can produce remarkable synergistic effect and has an excellent termite control effect; the pesticide effect is good, the drug toxicity is low, and the preparation is environment-friendly.

The invention relates to an intraocular inflammation resisting implant as well as a preparation method and application thereof. The intraocular inflammation resisting implant has a core-shell structure; a core layer is a cylindrical object formed by coextrusion by using a melt extrusion method after chlorogenic acid serving as an effective component and a drug carrier are mixed; a shell layer is a layer of electrostatic spinning membrane loading the chlorogenic acid wrapping the outer surface of the cylindrical object by using an electrospinning method after the chlorogenic acid serving as the effective component and the drug carrier are mixed. Compared with a traditional systematic administration mode, the intraocular inflammation resisting implant disclosed by the invention has the advantages that the systematic drug toxicity can be greatly reduced while the concentration of a local effective drug is guaranteed; importantly, the intraocular inflammation resisting implant disclosed by the invention has the core-shell structure, wherein the shell layer is an electrostatic spinning layer, and the release speed of the drug is faster; the core layer is a melt extrusion layer, and continuous release of the drug can be realized.

The invention provides a microcapsule-embedded multivesicle liposome drug carrier and a preparation method thereof, using sucrose solution as the inner water phase, glucose and L-lysine solution as the outer water phase, and lipid-dissolved methylene chloride solution For the oil phase, the inner water phase and the oil phase are homogenized and emulsified at high speed to form colostrum, then the outer water phase is added, vortex emulsified to form a double emulsion, and then the dichloromethane is evaporated by rotary evaporation to obtain a multivesicular liposome suspension; Multivesicular liposomes are dispersed into sodium alginate solution, dispersed evenly, and then dripped into calcium chloride solution through a high-pressure microcapsule forming device and a syringe pump to prepare calcium alginate embedded with multivesicular liposomes Glue beads. Finally, the gel beads and cationic polymer solution are formed into a film to obtain the sample; at least one of the multivesicular liposomes or the multivesicular liposomes in the microcapsules is loaded with drugs. The invention ensures the spatial distribution of different medicines, improves the long-term release and absorption of medicines, and reduces the toxic and side effects of simple use of medicines.

The invention belongs to the technical fields of RAFT polymerization, PEG-A monomer grafting and drug toxicity testing, and relates to a method for improving the water solubility of 7,8-dihydroxyflavanone based on a RAFT polymerization method. The method comprises the following steps: first performing chlorination reaction on a RAFT reagent, then grafting the RFAT reagent to the 7,8-dihydroxyflavanone, and finally performing RAFT polymerization reaction to graft PEG-A to the 7,8-dihydroxyflavanone. A process is simple and convenient to operate, the improvement effect on the water solubility of the 7,8-dihydroxyflavanone is obvious, the molecular weight is easy to control, the cost and the toxicity are low, and the polymerization degree can be accurately controlled by RAFT polymerization so as to synthesize 7,8-dihydroxyflavanone with different molecular weights and different polymerization degrees.

The present invention provides a pharmaceutical composition for treating or preventing the toxic effects of therapeutic drugs, and the use of N-acetylcysteine ​​(alone or in combination with a therapeutically effective amount or higher than the usual therapeutically effective amount) that can reduce the toxicity of the drug. used in combination with sexual agents) to achieve its therapeutic superiority. The present invention also provides a pharmaceutical composition for the treatment or prevention of the toxic effects of therapeutic drugs, and the use of N-acetylcysteine ​​(either alone, or with a therapeutically effective amount or higher than the usual dose of N-acetylcysteine) that can reduce toxicity. The therapeutic advantages of therapeutic agents) whose side effects are exacerbated by increased oxidative stress, or a treatment-related decrease in the patient's cysteine / glutathione levels, or may be Relieved by administration of NAC.