Anti malignant tumour southern radix bupleuri extract preparing method

Abstract

The invention is a kind of bupleurum root ext extracted from the southern bupleurum root and used to cure the hyperpiasia diseases and its method of extraction. The active components of the bupleurumroot ext at least includes the heterocyclic compound, with the Z or E position of the carbon 2(5), centered around the gamma- butyrolactone and the new bupleurum root lactone, isobupleurum root lactone, bupleurum root lactone similar and its ramification contained in the heterocyclic compound. From the results of the experiments of the drug toxicity, characteristics of the cell, animal experimentsand histologic section, we can know that the ext of the outhern bupleurum root can restrain the cancer cells of the liver cancer, lung cancer, oophoroma, large intestine cancer or swart brain glia cell tumour and specially kill the tomour cells with drug resistance to the taxales medicines after the chemotherapy. It can be a new resource of the drug screening of the tumor inhibitor.

Description

technical field [0001] The present invention relates to a kind of plant extract used in the field of cancer treatment, which is extracted from natural products to treat Cell Proliferative Disorder and its extraction method, especially about a plant extract isolated from Bupleurum scorzonerifolium ), and heterocyclic compounds with γ-Butyrolactone (γ-Butyrolactone) core as the treatment of human liver cancer (Hepatoma), lung cancer (Lung cancer), ovarian cancer (Ovarian cancer), malignant glioma (Human Malignant Glioblastoma) and colorectal cancer (Colorectal Cancer) and other cancer cells of the main active ingredient of South Bupleurum extract and its extraction method. Background technique [0002] Cancer, a cell proliferative disease, has become the top ten cause of death. According to the statistics of Boring et al. in 1993, about 526,000 people in the United States die of cancer every year. Taking breast cancer as an example, it has almost become the main killer of wom...

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Problems solved by technology

According to the research of Rao et al. in 1995, these tumor cells (such as the human lung cancer cell line AT-12) will change the six subunit configurations (Subunit Configuration) that make up the β microtubules, so that paclitaxel not only cannot interact with the β microtubules Combined, it will be excluded by the cells as a foreign substance (Ion-pumping), causing tumor cells to gradually develop resistance to paclitaxel and gradually lose efficacy in the later stages of chemotherapy

[0011] And, with existing other anticancer drugs, such as 5-fluoropyrimidine diketone (5-Fluorouracil), epothilone (Epothilone), cisdiammine dichloroplatinum (commonly known as Cisplatin), procarbazine (Procarbazine) ) and cyclophosphamide (Cyclophosphamide) alone or in combination with paclitaxel to treat patients who are resistant to taxanes, the clinical treatment effect is not good, and it is also found from the cell experiment that the current anticancer drugs are difficult to inhibit the paclitaxel resistance. The drug-resistant tumor cell line (Taxol-resistance TumorCell Line) proliferates. However, if the dose of the drug is increased, for example, when the dose of paclitaxel is increased to 300 mg per kg and injected into rats, the cytotoxicity of paclitaxel is very strong, often in After administration, it causes massive necrosis of normal cells (Necrosis).

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