109 results about "Desulfobulbus sp." patented technology

The present invention relates to a fast acting chemical treatment for preventing the generation of hydrogen sulfide odor by the microbial metabolic activities of sulfate reducing bacteria. Specifically, the invention relates to a method for preventing hydrogen sulfide odor generation in a sulfur species-containing aqueous medium, which includes adding to the aqueous medium an effective amount for the purpose of a sulfide scavenger treatment selected from the group consisting of glyoxal, triazine, n-chlorosuccinimide, and mixtures thereof.

The invention relates the microorganism agent used to control oil field sulfate-reducing bacteria and its application. The invention can used to control oil field secondary depletion sulfate-reducing bacteria and microorganism. The water outlet system of secondary depletion technology comprises sulfate-reducing bacteria, H2S and sulphide, which can cause corrosion and pollution of sulphide. Microorganism agent comprises denitrifying bacteria culture, nutrescin and synergic nutrescin. 0.01-1wt% denitrifying bacteria culture, 5-100g / L culture and 10-200ppm synergic nutrescin. Microorganism agent can avoid SRB growing, SRB producing H2S, and removing sulphide. At the same time, metabolite can improve recovery efficiency.

The invention belongs to the technical field of treatment of waste water, and particularly relates to a method for reduction of sulfate into sulfide and treatment of metal waste water by sulfate reducing bacteria growing up with electric current. The sulfate reducing bacteria provided by the invention is a mixing bacteria group, which comprises Desulfobulbus propinonicus, geobacter sulfurreducens. Under the condition of lacking organic carbon resource, an electrode is used as the only electronic donator for growing, sulfate is reduced into sulfide via bioelectrical chemistry, and the sulfide is applied to the treatment of metal waste water. According to the method provided by the invention, the defect that organic carbon resource is needed to be used as the electronic donator is overcome,the operation is simple, the secondary pollution is zero, the cost is low, and is particularly suitable for the treatment on organic carbon metal waste water of mine waste water, metal smelting wastewater, electric plating waste water and the like.

A method for quantitatively testing the water quality in oil field by use of sulfate reducing bacterium (SRB) includes taking 3-5 parallel samples in same dilution gradient, filling them in culture media, putting the MPN culture media on shaker, shaking at 38 deg.C and 180 rpm and respective counting in the fourth and the seventh days.

The invention relates to the field of water environment protection, in particular to sulfate reducing bacteria embedded particles for treating ionic rare earth mine wastewater as well as a preparationmethod and an application of the sulfate reducing bacteria embedded particles, and solves the problem that sulfate reducing bacteria in SRB treatment acid mine wastewater are poisoned by heavy metalsand insufficient carbon sources to influence the practical application effect. The embedded particle is of a multi-layer structure and is sequentially composed of a sulfate reducing bacteria biofilmculturing charcoal layer, a composite hydrolase slow-release carbon source layer, an adsorption layer and a cross-linking layer from inside to outside. The invention provides an embedded particle integrated double hydrolysis catalysis system, the release of a slow-release carbon source can be accelerated, an anaerobic environment required by reaction is provided, the toxic action of heavy metals and acid radical ions on sulfate reducing bacteria is reduced, the practical application effect is improved, and sulfate radicals and heavy metal ions in acidic mine wastewater and ionic rare earth mine wastewater can be effectively reduced.

The invention provides pipeline steel with sulfate reducing bacterium corrosion resistance performance so as to remarkably lower the possibility of sulfate reducing bacterium corrosion from the perspective of materials. The steel comprises the following chemical components of, by weight, 0.10% or less of C, 0.50% or less of Si, 2.0% or less of Mn, Cu and the balance Fe and inevitable impurities, wherein the weight percentage of the Cu meets the relation of 1.5%<=Cu<=4.0%; and the steel can further comprise one or more of 0.6% or less of Ni, less than 0.3% of Mo, smaller than 0.3% of Cr, smaller than 0.05% of N and smaller than 0.05% of Ti. According to the pipeline steel, the risk of sulfate reducing bacterium corrosion in soil can be obviously reduced, and the pipeline steel can be applied to production of steel for underground pipelines.

The present invention relates to a fast acting chemical treatment for preventing the generation of hydrogen sulfide odor by the microbial metabolic activities of sulfate reducing bacteria. Specifically, the invention relates to a method for preventing hydrogen sulfide odor generation in a sulfur species-containing aqueous medium, which includes adding to the aqueous medium an effective amount for the purpose of a sulfide scavenger treatment selected from the group consisting of glyoxal, triazine, n-chlorosuccinimide, and mixtures thereof.

The invention discloses a novel biological inhibitor for inhibiting activity of sulfate reducing bacteria in an oil well and a use method thereof in the field of production of an oil field. The inhibitor comprises the following components in parts by weight: 100 parts of sodium nitrite, 5-10 parts of sodium nitrate, 0.1-0.5 parts of sodium molybdate and 0.1-0.5 parts of cupric nitrate. The use method comprises the following steps of: (1) determining the concentration contents of SO4<2-> and S<2-> in produced water of the oil field; (2) converting dosage of the inhibitor; (3) preparing an inhibitor mother liquid described in claim 1 according to the dosage of sodium nitrite; and (4) adding the inhibitor mother liquid obtained in the step (3) into the shaft through a sleeve in an adding mode by the sleeve of the oil field. The corrosion rate of the produced water is lowered by using the inhibitor, and the production cost is reduced. Growth of source denitrifying bacteria in the well is activated, so that fast grow of SRB (Sulfate Reducing Bacteria) is effectively inhibited. The inhibitor can be used for inhibiting the activity of sulfate reducing bacteria in the oil well.

The invention relates to a method for demineralization of lakes and rivers, and discloses a method using sulfate-reducing bacteria for demineralization of lakes and rivers, which includes: step one, setting up of a water self-purification reinforcing system; step two, enrichment culture of sulfate reducing bacteria on culture medium; and step three, throwing, domesticating and fixing of the sulfate reducing bacteria. The method using the water self-purification reinforcing system in an A / O (anoxic-oxic) operating way can effectively reduce concentration of pollutants of sulfate radicals, organics, ammonia nitrogen and the like in water and reduce acidity of water.

Disclosed is an inhibitor applied in oil well for preventing against corrosion from sulfate reducing bacteria, which is characterized by comprising the following components: a. 0.05% by weight of aqueous solution of peptide coupling reagent (HOOBt) C7H5N3O2; b. 2.0-5.0% by weight of magnesium sulphate(MgSO4*7H2O); c. 1.5-4.5% by weight of sodium sulphate; d. 0.05-0.2% by weight of yeast extract; e. 0.5-2.0% by weight of dipotassium hydrogen phosphate(K2HPO4); f. 0.05-0.5% by weight of vitamin C; g. and the balance of water. During the process of preventing against corrosion from sulfate reducing bacteria in oil well, the inhibitor preventing against corrosion from sulfate reducing bacteria produced by the method can keep the content of sulfate reducing bacteria below 25 / ml in the case of 0.05-0.1% addition of the inventive inhibitor.

The invention relates to a technology for inhibiting SRB (sulfate reducing bacteria) in oilfield produced liquid, in particular to a biological bactericide for preventing and controlling the SRB in a high-temperature water body and an SRB inhibition method of the bactericide. The biological bactericide is JSHD-4 geobacillustoebii, has a sequence as shown in a sequence table SEQ ID NO:1, is collected in China General Microbiological Culture Collection Center (CCMCC) on March 5, 2013, and has a Latin literature name of Geobacillustoebii and a collection number of CGMCCNO7273. The JSHD-4 geobacillustoebii is subjected to microbial aerobic fermentation to prepare industrial fermentation liquid with the bacterial solution concentration of 1.0*10<6> to 1.3*10<10> cfu / mL, the tolerable temperature is 50-95 DEG C, and the optimal growth temperature is 60-70 DEG C. After the multifunctional biological bactericide prepared from the JSHD-4 geobacillustoebii is thrown into a well, nitrate reducing bacteria (DNB) are quickly proliferated in the high-temperature environment in the well, so that the biological bactericide is suitable for directly treating the SRB in the high-temperature environment in the well on line.

The invention relates to a denitrifying bacterium which can be used for effectively utilizing an inorganic nitrogen source and inhibiting growth and reproduction of sulfate reducing bacteria (SRB) and a fermenting production method of the denitrifying bacterium, belonging to the technical field of microbial fermentation engineering. The high-temperature denitrifying bacterium provided by the invention is preserved in CGMCC, the Latin name of the high-temperature denitrifying bacterium is Geobacillus pallidas, the preservation number is CGMCC NO 7270 and the preservation date is 5th, March, 2013. The high-temperature denitrifying bacterium provided by the invention can be used for effectively utilizing the nitrogen source and effectively inhibiting growth and reproduction of SRB under a high temperature condition of 45-90 DEG C. The denitrifying bacterium is simple in fermentation process and can be successfully fermented, and the culture medium is low in cost and short in culture period. The produced denitrifying bacterium is high in viable organism content and high in germ rate. The content of unit germ cells reaches 2*10<8>cfu / mL and the content of germs reaches over 90%.

Disclosed is the bio-desulphurization technology for conversing the sulfur compounds to the elemental sulfur, which can be used to remove the sulfur compounds in the smoke and waste water. The sulfite and sulfate are produced by dissolving the SO2 in the smoke in water; the sulfate reducing bacteria (SRB) deoxidize the sulfite and sulfate to sulfide under the anaerobic environment and the condition of external carbon source; then the sulfid is conversed to the elemental sulfur by the function of desulfuration microorganism (colorless thiobacilli) under the aerobic condition, and then the sulfur element can be removed from the system after deposition and separation. The invention achieves the recovery of elemental sulfur through the efficient biological reaction, thus effectively solving the pollution problem of SO2, realizing resource recovery of waste, and finally achieving the unification of environmental benefits and economic benefits.

The invention belongs to the technical field of wastewater biological treatment and discloses an immobilized sulfate reducing bacterium particle preparation method and application. The preparation method includes steps: adding graphene oxide sol into a sulfate reducing bacterium culture medium, stirring and mixing; adding sulfate reducing bacteria liquid into the sulfate reducing bacterium culturemedium, stirring and mixing; culturing for 18-72h at 10-45 DEG C in a pH value of 2-12 under anaerobic conditions to obtain particles namely immobilized sulfate reducing bacteria particles. Accordingto the preparation method, reduced graphene oxide serves as a biological carrier for immobilizing sulfate reducing bacteria, activity of the sulfate reducing bacteria is improved, and influences of heavy metals on the sulfate reducing bacteria are weakened; in addition, the preparation method is simple and convenient, high stability of the immobilized sulfate reducing bacteria in products is achieved, and extensive popularization and utilization can be realized.

An SRB (sulfate reducing bacteria) inhibitor for an oil storage tank comprises components in parts by mass as follows: 60-100 parts of glutaraldehyde or acrolein aldehydes, and or 30-70 parts of dodecyl dimethyl benzyl ammonium bromide or dodecyl dimethyl benzyl ammonium chloride, 15-20 parts of lysozyme, 20-30 parts of protease, 10-20 parts of diorthophthalic ethanediamine acetate and / or 10-20 parts of ethylene diamino-disuccinic acid salt and 5-10 parts of tetrakis hydroxymethyl phosphonium sulfate. The invention further discloses a method for using the SRB inhibitor for the oil storage tank, that is, the inhibitor is added according to the concentration of S<2-> in sedimentation water and mineralization degree. The inhibitor has the characteristics of small dosage, convenience in use, wide sources, low toxicity and the like and has double effects of sterilization and corrosion prevention.

The invention discloses a quantificational detection method for adhesive sulfate reducing bacteria and a biomembrane sampling device thereof, which relate to the quantificational detection method for sulfate reducing bacteria and the special biomembrane sampling device in the method. The invention solves the problem that the prior detection method fails to realize quick and more accurate quantificational detection of the adhesive sulfate reducing bacteria. The method has the main steps of: biomembrane removal by using the biomembrane sampling device, bacteria preparation, multiple proportion dilution, PCR operation, augmentation, electrophoretic detection, and meter reading and number recording. A probe (1) orderly passes through a probe compression assembly (5) and a body (4), and is fixed on the upper end surface of the body (4) through a fixing part (2), a shoulder (1-1) at the upper end of the probe (1) is lapped on the upper end surface of the probe compression assembly (5), and a control valve (3) is arranged on the side wall of the lower end of the body (4). The method has more accurate number recording and shortens counting time; the application of the device can effectively obtain adhesive biomembranes of tank walls and pipe walls.

The invention provides a microbial remediation method for heavy metal contaminated soil in a retired slag yard of a smelting plant. The method comprises the following steps of: selecting mixed bacteria of sulfate reducing bacteria and iron reducing bacteria, culturing in a liquid culture medium containing Zn<2+>, Pb<2+> and Cr<6+> under a neutral condition until the concentration of Zn<2+>, Pb<2+> and Cr<6+> in the culture medium is lower than that of a detection line, selecting strains in a bottle for transferring, and gradually increasing the concentration of Zn<2+>, Pb<2+> and Cr<6+> for gradient domestication; and adding the domesticated bacterial liquid into polluted soil, wherein the addition amount of the bacterial liquid is that the mass volume ratio (g: mL) of the soil to the bacterial liquid is 1: 1-3: 1. After culture is performed for 4-7 days at the temperature of 30-35 DEG C, the soluble Zn<2+>, Pb<2+> and Cr<6+> in the remedied soil are lower than 0-0.05 mg / L, 0-0.05 mg / L and 0-0.05 mg / L, and the groundwater quality standard III-type water standard is met.

The invention discloses a method of removing sulfate radical (SO4<2->) and hexavalent chromium (Cr(VI)) from industrial waste water through sulfate reducting bacteria (SRB) activated sludge-[alpha]-Fe2O3 coupling process and belongs to the technical field of salt-containing heavy metal waste water treatment. The method includes the following steps: a) cultivating SRB activated sludge with a modified Postgate's B culture medium in a batch reactor in a batch operation manner; b) after precipitation, discharging supernatants to obtain concentrated SRB activated sludge; c) mixing the waste water containing SO4<2-> and Cr(VI) with the concentrated SRB activated sludge, adding [alpha]-Fe2O3 (in the size of 30 nm) into the mixture to remove the SO4<2-> and Cr(VI) at the removal rate of 96.5% and 99.9% respectively; and d) mixing the supernatants with H2O2 and adding a catalyst Cu2O (in the size of 50 nm) to remove the S(-II) in the supernatants, wherein the removal rate of the S(-II) is 100%. The invention provides the high-efficient and low-cost method of removing SO4<2-> and Cr(VI) from the industrial waste water, and meanwhile because of the adsorption effect of Cu2O, elemental sulfur in discharged water can be enriched and recycled.

The invention discloses an inorganic powder for inhibiting sulfate-reducing bacteria by using tourmaline-loaded manganese and a preparation method thereof, which relates to a bacteriostatic agent and a preparation method thereof. It solves the problems of poor safety, poor heat resistance, easy decomposition and short service life of existing organic antibacterial agents. The invention is made of 60-99.99% of tourmaline and 0.01-40% of potassium permanganate solid or manganese chloride solid or the mixture of potassium permanganate solid and manganese chloride solid according to mass percentage. The preparation method is: use nanometer or submicron tourmaline to stir in deionized water; add nitrate solution; add potassium permanganate solid or manganese chloride solid or a mixture of potassium permanganate solid and manganese chloride solid and a dispersant , stirring; decompression suction filtration separation, cleaning with deionized water; drying the powder; grinding and calcining the powder and grinding the powder again to obtain an inorganic antibacterial powder. The invention not only effectively increases the amount of manganese loaded, but also improves the bactericidal effect of the bacteriostatic agent.

The invention provides a sterilizing agent for an oilfield water treatment system as well as a preparation method and application thereof. The sterilizing agent is prepared from the following components of 5wt.% to 60wt.% of dodecyl dimethyl benzyl ammonium halide, 0.5wt.% to 5wt.% of D-amino acid, 10wt.% to 30wt.% of surfactant, 3wt.% to 10wt.% of buffer agent and the balance of water. The sterilizing agent has the advantages that the sterilizing efficiency is high, the efficient sterilizing property on SRB (sulfate reducing bacteria) and TGB (total general bacteria) is realized, the durationof effect is long, the production process is simple, and the cost is low.

The invention discloses a biological type sulfurized zero-valent iron material and a preparation method and application thereof, belonging to the technical field of heavy metal pollution remediation. According to the invention, a template material is utilized, a biological reduction method and a chemical reduction method are combined, and the sulfurized zero-valent iron material is synthesized under the combined action of sulfate reducing bacteria and a reducing agent. Besides, in a synthesis process, sulfate reducing bacteria and metabolites thereof are loaded on the surface and in pores of the template material, so various active groups are added to the material, and the adsorption effect of the material on metal ions is enhanced. Compared with a material synthesized by a direct chemical method, the sulfurized zero-valent iron material prepared by the method has better electron transfer performance, adsorption performance and dispersion performance. The raw materials adopted by the invention are environment-friendly, a material preparation process is simple, cost is low, adsorption efficiency is high, and heavy metals in the soil and a water body can be restored at the same time.

The invention discloses a biological enhancement application method of sulfate reducing bacteria. The method is characterized in that urease-producing strains are adopted to enhance the heavy metal immobilization capacity, a mixed bacteria culture solution is added into cadmium-containing wastewater, and closed culture is performed to obtain treated CdS precipitate. The urease-producing strain adopted by the invention has a partial symbiotic relationship with sulfate reducing bacteria, and consumes O2 to provide an expected anaerobic environment for the SRB strain while jointly participating in microbial precipitation to improve the heavy metal immobilization efficiency. Meanwhile, pollution of H2S in a cultured bacterial solution to the environment is reduced while sulfur ion S2-oxidizingbacteria growth is inhibited. In a waste liquid containing 20mg / L CdCl2, the cadmium removal rate reaches 99.0%, and the method has a good cadmium removal effect and can be widely applied to treatment of heavy metal-containing water sources and soil.

The invention provides an efficient compound sterilization corrosion inhibitor capable of inhibiting and decomposing a biological film and for a water system. The efficient compound sterilization corrosion inhibitor is prepared from the following components in percentage by weight: 0.003 to 0.01 percent of quaternary phosphonium salt, glutaraldehyde and / or cecropin, 0.0001 to 0.005 percent of D-amino acid and the balance of water, wherein the D-amino acid can be used for inhibiting and decomposing the biological film attached on a metal surface and the biological film is converted into a floating sate from a fixation state and microorganisms can be easily killed through fungicide, so that the sterilization effect of the fungicide can be remarkably enhanced and the corrosion caused by the microorganisms is alleviated to the great extent; all the components of the compound sterilization corrosion inhibitor have a synergistic effect and have an extremely excellent sterilization corrosioninhibition effect; the killing rates on sulfate-reducing bacteria, iron bacteria and saprophytic bacteria are greater than or equal to 99 percent and the corrosion inhibition rate is greater than or equal to 80 percent; the disadvantages of traditional fungicide of large dosage, high cost, great toxicity and heavy environment burdens are overcome.

The invention discloses an integrated vertical high-concentration sulfate wastewater treatment device. The device comprises a hydrolytic acidification reaction section, a hydrogen sulfide stripping section, a methanogenesis reaction section and a tank body, wherein the hydrolytic acidification reaction section is arranged at the lower section of the tank body, and includes a bottom water distribution pipe used for uniformly distributing high-concentration sulfate wastewater and a first filler layer used for providing a growth environment for sulfate reducing bacteria; the hydrogen sulfide stripping section is arranged at the middle section of the tank body and comprises a stripping pipeline and a gas collecting part; and the methanogenesis reaction section is arranged at the upper sectionof the tank body and comprises a second filler layer for providing a growth environment for methanogens and an effluent weir arranged along the circumference of the tank body. According to the invention, the anaerobic reaction is divided into the hydrolytic acidification reaction section and the methanogenesis reaction section, and the hydrogen sulfide stripping section is additionally arranged between the hydrolytic acidification reaction section and the methanogenesis reaction section, so that the inhibition effect of hydrogen sulfide on methanogens is weakened, the activity of the methanogens is guaranteed, and the treatment effect on high-concentration sulfate wastewater is improved.

The invention relates to the technical field of anticorrosive paint, in particular to a sulfate reducing bacteria metabolism microenvironment response type intelligent coating and a preparation method thereof. The sulfate reducing bacteria metabolism microenvironment response type composite coating comprises an antibacterial surface layer and an anti-corrosion bottom layer, nano particles for packaging a bactericide are doped in the antibacterial surface layer, and nano particles for packaging a corrosion inhibitor are doped in the anti-corrosion bottom layer; the nanoparticles are sulfate reducing bacteria metabolism microenvironment response type nanoparticles. The sulfate reducing bacteria metabolism microenvironment response type nanoparticles have a sulfur ion response release function. The bactericide or the corrosion inhibitor is packaged in the framework structure of the nano particles, uncontrollable leakage is avoided, when sulfate reducing bacteria are massively propagated and metabolized to generate sulfur ions, and metal tends to be corroded, the nano particles can respond to the increased sulfur ion concentration in the corrosion microenvironment and decompose the sulfur ions, so that the timed, fixed-point and quantitative controlled release of the functional molecules is realized, and the problems of cost and environment caused by uncontrollable release of the functional molecules are avoided.

The invention discloses a method for treating acidic mine wastewater by micro-electrolysis enhanced sulfate reducing bacteria and a micro-electrolysis bioreactor. By filling a reactor with an iron-carbon micro-electrolysis filler and inoculating sulfate reducing bacteria, part of metal ions in the acidic mine wastewater is rapidly reduced through a micro-electrolysis reaction between iron and carbon, the pH of wastewater is increased, and the problem that sulfate reducing bacteria are inhibited and harmed by low pH and heavy metals is solved; the sulfur reduction rate of sulfate reducing bacteria is increased through electrical stimulation generated by micro-electrolysis, and part of metals is removed in a sulfide precipitation form; Fe<3+> and alkalinity are generated, part of metals is removed in a Fe(OH)3 coagulation and hydroxide precipitate mode. Compared with other bioreactors, the micro-electrolysis bioreactor has the advantages of high sulfate and heavy metal removal efficiencyand high system stability.

The invention discloses a double quaternary ammonium salt type gas field reinjection water bactericide and a bactericide composition comprising the bactericide. Compared with a traditional bactericide 1227, the double quaternary ammonium salt provided by the invention has higher positive charge density, and the capacity of absorbing negatively charged bacterial cell surfaces is improved. Therefore, the bactericide provided by the invention has relatively high antibacterial activity. Also, the bactericide has the advantages of long effect duration and low foam. The double-long-chain quaternary ammonium salt has one hydrophilic group and two hydrophobic groups, such that higher capacity for reducing surface tension is provided, and water solubility can be improved. Under a condition of relatively high water hardness, good solubility and stability are shown. With the gas field reinjection water bactericide composition, a bacteria test is carried out according to an extinction dilution method represented by SY / T0532-93, and bactericidal experiments are carried out aiming at sulfate-reducing bacteria (SRB), iron bacteria (FB) and saprophytes (TGB). As a result, an effective bactericidal concentration is 20-30ppm, and a bactericidal rate reaches 99.9% and higher, such that reinjection requirements are satisfied.

The invention belongs to the technical field of microbial oil displacement, and particularly relates to a directional adjustment and control method for oil reservoir endogenous functional microbes. The method specifically comprises the following steps of screening a test oil reservoir, separating and amplifying sulfate reducing bacteria phages, screening the sulfate reducing bacteria phages, screening a directional adjustment and control activator system, evaluating oil displacement performance of the directional adjustment and control activator system, performing a field test and performing effect evaluation. The method disclosed by the invention has the advantages of being reasonable, simple in process, simple to operate, safe and reliable, less in investment, low in cost and good in field test effect, reduces the cost by 30% or more, and improves the recovery efficiency by 20% or more according to the field test.

The invention relates to a preparation method of an electrothermal film, and belongs to the technical field of electrothermal materials. A metal chloride is prepared into a solution, dopamine is used to modify the metal chloride solution to obtain a mixed solution of the electrothermal film, graphene, gum and sulfate reducing bacteria are mixed for fermentation, under the effect of microbes, the graphene is combined with the gum, a binding force with resin is improved, nitrogen and sulfur elements are introduced into graphene molecules to enhance the stability of the graphene film, the mixed solution of the electrothermal film is injected on the basal body and serves as a bottom film, and one graphene layer is combined to obtain the electrothermal film. The prepared electrothermal film is high in stability and adhesive force, tends not to fall off the basal body, and has wide application prospects.