Immobilized sulfate reducing bacterium particle preparation method and application
A sulfate and granule technology, applied in biochemical equipment and methods, chemical instruments and methods, and microbial-based methods, can solve problems such as slow reaction, high energy consumption, and poor effluent quality, so as to promote growth and activity, Good settling performance, accelerated removal effect
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Embodiment 1
[0029] The present embodiment provides a kind of preparation method of immobilized sulfate-reducing bacteria particle, such as figure 1 As shown, it includes the following steps in turn:
[0030] S1) Add 0.2ml of graphene oxide sol into 50ml of sulfate-reducing bacteria culture medium, that is, the volume ratio of the two is 0.4%, and stir and mix;
[0031] S2) The pH value of the culture medium is adjusted to 7.5, and 0.5ml of sulfate-reducing bacteria bacterial liquid (concentration is 2.1×10) is inoculated into the sulfate-reducing bacteria medium. 10 cfu / ml), the volume ratio of sulfate-reducing bacteria bacterium liquid and sulfate-reducing bacteria culture medium is 1%, stirring and mixing;
[0032] S3) Infuse nitrogen gas for 20 minutes to ensure an anaerobic culture environment. After sealing, culture at 30° C. for 18 hours and enter a stable period. The obtained black particles are BY-rGO particles.
Embodiment 2
[0034] The present embodiment provides a kind of preparation method of immobilized sulfate-reducing bacteria particle, and it is similar to embodiment 1, and difference is: in S1) 0.4ml graphene oxide sol is added in the sulfate-reducing bacteria culture medium of 50ml, promptly two The volume ratio of the latter is 0.8%.
Embodiment 3
[0036] The present embodiment provides a kind of preparation method of immobilized sulfate-reducing bacteria particle, and it is similar to embodiment 1, and difference is: in S1) 0.6ml graphene oxide sol is added in the sulfate-reducing bacteria culture medium of 50ml, promptly two The volume ratio of the latter was 1.2%.
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