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Quantitative determination method of attached type sulfate reducing bacteria and used biological film sampling apparatus

A quantitative detection method and technology of sampling device, which are applied in the measurement/inspection of microorganisms, biochemical equipment and methods, and methods of sampling biological materials, etc., can solve the problem of high cost, shorten the counting time, have a simple structure, and reduce the detection cost. Effect

Inactive Publication Date: 2008-11-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problem that the existing sulfate-reducing bacteria detection method cannot realize fast and accurate quantitative detection of attached sulfate-reducing bacteria, and the cost of the existing sulfate-reducing bacteria detection method is relatively high; and provides a Quantitative detection method of a kind of attached type sulfate reducing bacteria and biofilm sampling device used

Method used

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  • Quantitative determination method of attached type sulfate reducing bacteria and used biological film sampling apparatus
  • Quantitative determination method of attached type sulfate reducing bacteria and used biological film sampling apparatus
  • Quantitative determination method of attached type sulfate reducing bacteria and used biological film sampling apparatus

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specific Embodiment approach 1

[0010] Embodiment 1: The method for quantitative detection of attached sulfate-reducing bacteria described in this embodiment is realized according to the following steps: Step 1. Remove the sulfate-reducing bacteria that are attached to the inner wall of the tank or pipe with a biofilm sampling device. Bacterial biofilm: First, punch a hole on the side wall of the tank or pipe, then install the biofilm sampling device at the through hole, and make the probe on the biofilm sampling device and the inner side of the tank or pipe The wall is flush and kept on-line for 10-20 days. After the sulfate-reducing bacteria fully form a film on the probe, the biofilm can be removed; Step 2, preparation of sulfate-reducing bacteria: place the probe on the The biofilm was peeled off to the prepared solution to make bacteria; Step 3, the bacteria prepared in Step 2 were diluted; Step 4, PCR operation on ice; Step 5, PCR on the amplification instrument Amplification; step six, the amplified p...

specific Embodiment approach 2

[0011] Specific embodiment two: the method for quantitative detection of attached sulfate-reducing bacteria described in this embodiment is realized according to the following steps:

[0012] Step 1. Use a biofilm sampling device to remove the biofilm containing sulfate-reducing bacteria attached to the inner wall of the tank or pipe: first drill a hole on the side wall of the tank or pipe, and then install the biofilm sampling device on the Make the probe on the biofilm sampling device flush with the inner side wall of the tank or pipe at the through hole, and keep it installed online for 10-20 days. After the sulfate-reducing bacteria fully form a film on the probe, take lower biofilm;

[0013] Step 2. Preparation of sulfate-reducing bacteria cells: peel off the biofilm on the probe into the prepared solution to make cells; specifically include the following steps: a. Put the removed biofilm into a 1mL container deionized water and sterilized 1.5mL centrifuge tube; b. Fully...

specific Embodiment approach 3

[0019] Embodiment 3: In this embodiment, the biofilm sampling device described in step 1 is kept online and installed on the side wall of the tank or pipe for 15 days. Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a quantificational detection method for adhesive sulfate reducing bacteria and a biomembrane sampling device thereof, which relate to the quantificational detection method for sulfate reducing bacteria and the special biomembrane sampling device in the method. The invention solves the problem that the prior detection method fails to realize quick and more accurate quantificational detection of the adhesive sulfate reducing bacteria. The method has the main steps of: biomembrane removal by using the biomembrane sampling device, bacteria preparation, multiple proportion dilution, PCR operation, augmentation, electrophoretic detection, and meter reading and number recording. A probe (1) orderly passes through a probe compression assembly (5) and a body (4), and is fixed on the upper end surface of the body (4) through a fixing part (2), a shoulder (1-1) at the upper end of the probe (1) is lapped on the upper end surface of the probe compression assembly (5), and a control valve (3) is arranged on the side wall of the lower end of the body (4). The method has more accurate number recording and shortens counting time; the application of the device can effectively obtain adhesive biomembranes of tank walls and pipe walls.

Description

technical field [0001] The invention relates to a quantitative detection method of sulfate-reducing bacteria and a special biofilm sampling device in the method. Background technique [0002] At present, the detection methods of sulfate-reducing bacteria (SRB) mainly focus on three levels: one is the traditional detection method, including culture method and microscope direct counting method. The bacteria in the sample are cultured to count the live bacteria, mainly including the plate colony count method, the most probable number (MPN), also known as the multiple dilution method, etc.; and the microscope direct count method is timely, Quickly and accurately determine the total amount of bacteria, mainly including optical microscope, fluorescence microscope and other technologies, turbidity (nephelometric) counting method, electrical impedance method and flow cytometry method; the second is the genetic level, based on the SRB species Qualitative detection of 16S rDNA sequen...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12Q1/68C12M1/26
Inventor 魏利马放杨基先邱珊侯宁李大鹏
Owner HARBIN INST OF TECH
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