The invention relates to a preparation method of kiwi fruit ice cream. The preparation method comprises the following specific steps of (1) preparing kiwi fruit powder: cleaning fresh kiwi fruits, peeling the cleaned kiwi fruits, cutting the peeled kiwi fruits into kiwi fruit slices, freeze-drying the kiwi fruit slices, crushing the freeze-dried kiwi fruit slices, and screening the crushed kiwi fruits so as to obtain kiwi fruit powder; (2) mixing raw materials with auxiliary materials: mixing the kiwi fruit powder with vegetable fat powder, nonfat dry milk powder, white granulated sugar, monostearin, sucrose esters, guar gum, xanthan gum and yolk liquid, adding water, and performing sufficient stirring; (3) performing high-pressure homogenizing: homogenizing a mixture obtained by uniformly mixing the raw materials with the auxiliary materials through a second-class high-pressure homogenizing machine; (4) performing sterilization, and performing filtration; (5) performing cooling; (6) performing ageing; (7) performing congelation; (8) performing filling, and performing shaping; and (9) performing hardening. The kiwi fruit ice cream made by the preparation method disclosed by the invention is viridescent in color, moderate in sugariness, fine and smooth in mouth feel, intact in shape maintaining, and rich in various nutrient components particularly vitamins, and has specific taste and medical health-care value of kiwi fruits.