35 results about "Aldehyde Reductase" patented technology

The present invention relates to a method for identifying therapeutic agents for reducing and monitoring the growth, erosion, rupture or stability of an atherosclerotic plaque comprising the analysis of the differential expression of at least two genes coding proteins chosen among among Stearoyl CoA desaturase, Phosphatidic acid phosphate, and Phosphoinositide-specific-phospholipase-B1, eventually in association with the analysis of the differential expression of at least one gene coding a protein choosen in the group comprising Aldose reductase and aldehyde reductase, Sphingomyelinase, Acid ceramidase, Ceramide glucosyl transferase, Sphingosin phosphate liase, Thymosine beta 4, Aldehyde dehydogenase, ATPase Ca++ binding protein and CD163.

The invention discloses recombination klebsiella pneumonia capable of co-producing 3-HP and P3HP, and a preparation method and application thereof. The recombination bacterium is obtained by introducing glycerol dehydratase gene, glycerol dehydratase reactivation enzyme gene, aldehyde dehydrogenase gene, propionyl coenzyme A synthetase gene and polyhydroxyalkanoate synthetase gene into a host recombination klebsiella pneumonia in which 1, 3-propylene glycol oxidoreductase gene and aldehyde reductase / alcohol dehydrogenase gene are knocked out. According to the technical scheme, the production cost of 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) is reduced, and 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) can be synthesized at the same time by taking a same thallus as the host.

The invention discloses a method for preparing optical activity chiral secondary alcohol by using a rhodotorula reducing enzyme preparation agent. The rhodotorula reducing enzyme preparation agent is extracted from a rhodotorula cell, and comprises aldehyde reductase and mannitol dehydrogenase. Through the coupled chemical reaction of the aldehyde reductase and the mannitol dehydrogenase, mannite which is low in price can be used as cosubstrate, so that the in-situ regeneration and the cyclic utilization of coenzyme NADPH are realized during the reducing reaction process of a carbonyl group. The complete and highly-selective reduction of the high-concentration carbonyl substrate can be realized only through adding the coenzyme with catalytic amount in a synthesized reaction system. The production process is simple, the product is easy to extract and purify, and the rhodotorula reducing enzyme preparation agent can be used for preparing the chiral secondary alcohol with various high optical purity, in particular to (S)-aromatic secondary alcohol.

The invention relates to a method for producing the optically active alkanols of formula (I), wherein n is an integer of from 0 to 5; Cyc represents an optionally substituted, mononuclear or polynuclear, saturated or unsaturated, carbocylic or heterocyclic ring, and R1 represents halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, wherein R2, R3 and R4 independently represent H or a lower alkyl or lower alkoxy group and X− represents a counterion. According to the invention, an enzyme (E) selected from the groups of dehydrogenases, aldehyde reductases and carbonyl reductases is incubated in a medium containing the alkanone of formula (II), wherein n, Cyc and R1 are defined as above, in the presence of reduction equivalents. The compound of formula (II) is enzymatically reduced to the compound of formula (I) and the reduction equivalents consumed during reaction are regenerated by reacting a sacrificial alcohol to the corresponding sacrificial ketone using enzyme (E) and at least partially removing the sacrificial ketone from the reaction medium, and then isolating the product (I) so produced.

A method for synthesize pheromone fatty alcohol acetate ester based on three intermediate product of fatty acid metabolism pathway in microorganism includes such steps as high expression of fatty acidacyl ester in microorganism, high expression of fatty acid acyl ester in microorganism, high expression of fatty acid acyl ester in microorganism, high expression of fatty acid acyl ester in microorganism, high expression of fatty acid acyl ester in microorganism, high expression of fatty acid acyl ester in microorganism, and high expression of fatty acid acyl ester in microorganism. ACP reductase AAR, carboxylic acid reductase CAR, acyl-CoA reductase FAR and aldehyde reductase AHR, respectively. ACP, fatty acid or acyl-CoA producing fatty alcohol. At last, the pheromone fatty alcohol acetatewas synthesized by the reaction of fatty alcohol and acetyl-CoA with high expression of alcohol acetyltransferase ATF1 in fatty alcohol-producing engineering bacteria. The invention can realize microbial conversion from glucose to fatty alcohol acetate, and provides a feasible path for large-scale biosynthesis of fatty alcohol acetate.

The invention discloses recombinant expression of aldehyde reductase and an application thereof in bioconversion of glycerol into 1, 3-propylene glycol, belonging to the technical field of biological engineering. The invention is characterized in that a recombinant expression vector containing aldehyde reductase gene sequence is used to express the enzyme to a host cell for preparing 1, 3-propylene glycol. The beneficial effects and benefits of the invention are that recombinant Klebsiella (Pdk-yqhD) takes glycol as substrate to ferment for 30h, and the concentration of 1, 3-propylene glycol in the fermentation liquor is improved by 17.1% in comparison with a comparison strain; compared with the original strain, by using the recombinant restrain in the invention for fermentation, the yield of 1, 3-propylene glycol is improved by 10-50%, which lays a foundation on constructing genetically engineered microorganism of the high yield of the 1, 3-propylene glycol taking glycol as substrate.

The invention discloses recombination klebsiella pneumonia capable of co-producing 3-HP and P3HP, and a preparation method and application thereof. The recombination bacterium is obtained by introducing glycerol dehydratase gene, glycerol dehydratase reactivation enzyme gene, aldehyde dehydrogenase gene, propionyl coenzyme A synthetase gene and polyhydroxyalkanoate synthetase gene into a host recombination klebsiella pneumonia in which 1, 3-propylene glycol oxidoreductase gene and aldehyde reductase / alcohol dehydrogenase gene are knocked out. According to the technical scheme, the production cost of 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) is reduced, and 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) can be synthesized at the same time by taking a same thallus as the host.

The invention discloses a genetic engineering strain capable of coproducing isoprene and 1,3-propylene glycol and an establishment method and application thereof, and belongs to the technical field of genetic engineering. In the genetic engineering strain, a glycerol dehydratase gene and a glycerol dehydratase gene reactivating enzyme gene are integrated, a glycerol kinase gene is knocked out, and HMG-CoA reductase, HMG-CoA synthetase, mevalonate kinase, phosphomevalonate kinase, pyrophosphomevalonate decarboxylase, IPP isomerase, isoprene synthetase, aldehyde reductase and transhydrogenase are overexpressed. According to the invention, through genetic engineering, a metabolic way capable of coproducing the isoprene and the 1,3-propylene glycol is successfully established in escherichia coli; by the establishment method, redox cofactors in cells of the genetic engineering strain are metabolized in a balanced way, and the yield in coproducing the isoprene and the 1,3-propylene glycol through glucose and glycerol fermentation is increased.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductase thereby increasing the production of vanillin or vanillin beta-D-glucoside.

Aldehyde and ketone reductase crude enzyme system catalytic synthesis of recombinant bacteria ( S )‑N,N‑Dimethyl‑3‑hydroxy‑3‑(2‑thiophene)‑1‑propylamine (( S )‑DHTP), which belongs to the technical field of biocatalytic asymmetric transformation. The present invention is to add auxiliary substrate and initial amount of coenzyme NADP in recombinant bacterial crude enzyme system + Promote the regeneration cycle of the coenzyme NADPH in the system, the reaction substrate is N,N-dimethyl-3-ketone-3-(2-thiophene)-1-propanamine (DTKP), and the recombinant bacteria express the aldehyde and ketone reductase gene of E. coli BL21(DE3)(pETCPAR4), aldehyde and ketone reductase gene cpar4 from Candida parapsilosis ( Candida parapsilosis ) CCTCC NO: M 203011, the gene encodes aldehyde and ketone reductase CPAR4, which catalyzes the asymmetric reduction of DKTP to ( S )‑DHTP. The invention utilizes the cell-free system to catalyze the reaction without additionally adding coupling enzymes required for coenzyme regeneration in the reaction system, improves the efficiency of the direct action of the enzyme and the substrate, shortens the reaction time, and obtains better conversion effects.

The invention relates to the technical field of biochemical engineering, and particularly discloses a method for producing 1, 6-hexanediol through whole-cell catalysis, a recombinant microorganism and application of the recombinant microorganism. Compared with an original strain, the recombinant microorganism provided by the invention has the advantages that aldehyde reductase yahk is overexpressed, carboxylic acid reductase MpCAR and 4 '-phosphoric acid panthenyl aminotransferase sfp are expressed, and the original strain is escherichia coli. The recombinant Escherichia coli obtained by introducing exogenous carboxylic acid reductase and 4 '-phosphoric acid panthenotransferase into Escherichia coli and overexpressing aldehyde reductase is used for whole-cell catalysis, 1, 6-hexanediol can be produced from 1, 6-adipic acid under mild conditions, and the method has a very wide application prospect.

The invention discloses a method for synthesizing pheromone fatty alcohol acetate in microorganisms based on three intermediate products of fatty acid metabolism pathway as precursor molecules. The reductase CAR, fatty acyl-CoA reductase FAR and aldehyde reductase AHR are used to obtain engineering bacteria that can reduce fatty acyl-ACP, fatty acid or fatty acyl-CoA to produce fatty alcohol, respectively. Finally, alcohol acetyltransferase ATF1 was highly expressed in fatty alcohol-producing engineering bacteria, which was used to catalyze the reaction of fatty alcohol and acetyl-CoA to synthesize pheromone fatty alcohol acetate. The invention can realize the microbial transformation from glucose to fatty alcohol acetate, and provides a feasible way for large-scale biosynthesis of fatty alcohol acetate.

The present invention discloses hyoscyamine aldehyde reductase synthase (hyoscyamine aldehyde reductase synthase, HAR) and its application, which has the amino acid residue sequence shown in SEQ ID NO.4 and the nucleotide sequence shown in SEQ ID NO.3; The hyoscypolamine reduction reaction product catalyzed by prokaryotic expression is hyoscypolamine, and the hyoscypolamine content in belladonna cell lines can be increased after the hyoscypolaldehyde reductase is used to transform belladonna cell lines. Significance.

The invention relates to genetic recombination saccharomyces cerevisiae with a detoxification function. The genetic recombination saccharomyces cerevisiae contains an exogenous aldehyde reductase gene, and the exogenous aldehyde reductase gene is an aldehyde reductase gene of which the sequence is shown in SEQ ID NO:1. The exogenous aldehyde reductase gene is obtained in a way that genome DNA of pichia stipitis is taken as a template for PCR amplification, and meanwhile, two enzyme digestion sites including Xho I and Not I are introduced into the terminal C and the terminal N respectively. A GRE2 gene segment is connected to a pYES2 / NTA plasmid vector to obtain a recombinant plasmid pYES2 / NTA-GRE2, and then the recombinant plasmid pYES2 / NTA-GRE2 is transformed into the saccharomyces cerevisiae to obtain the genetic recombination saccharomyces cerevisiae with the detoxification function. The saccharomyces cerevisiae can efficiently ferment pretreatment materials of lignocellulose to produce fuel ethanol; meanwhile, inhibitory or toxic aldehyde substances generated in the fermentation process can be removed, and detoxification of fermentation inhibiting substances in the pretreatmentmaterials of the lignocellulose is achieved, so that it is ensured that the process of producing the ethanol through fermentation of the cellulose is smooth and efficient.

A method for biosynthesis of polymer precursors, including, adipic acid, 1,6-hexanediol, 6-hydroxyhexanoic and 6-aminocaproic acids from carboxylic acids is provided. A method for biosynthesis of adipic acid from six-carbon dicarboxylic acids having α, β-enoate reductase activity by treatment with an enzyme is provided. The biocatalytic conversion of aliphatic and hydroxycarboxylic acids to corresponding aldehydes, alcohols, and amines using novel carboxylate reductases, aldehyde reductases, and aminotransferases is described. Also provided are genetically engineered microorganisms for use in the biosynthetic processes.

The invention discloses a recombinant bacterium for synthesizing D-lactic acid by adopting the biodiesel by-product, namely, glycerol and applications of the recombinant bacterium, belonging to the field of genetic engineering. For the recombinant bacterium provided by the invention, the dehydrogenase gene of D-lactic acid, namely, ldhA is introduced into the host, namely, recombinant Klebsiella pneumoniae with the 1,3-propanediol oxidoreductase gene dhaT and the aldehyde reductase / alcohol dehydrogenase gene yqhD knocked out, and thus the engineering strain is obtained. D-lactic acid is synthesized by taking the biodiesel by-product, namely, glycerol as the substrate, in the shake flask level, the yield and conversion rate respectively achieve 12.7g / L and 50.4%, the optical purity is 100%, and the recombinant bacterium has the advantages that the optical purity and conversion rate are high, and the production cost is low.