43 results about "Carboxylic acid reductase" patented technology

The present invention provides methods of enzymatically preparing aldehydes from fatty acids by utilizing a carboxylic acid reductase enzyme to reduce the fatty acids to their corresponding aldehydes. The present invention also provides aldehydes prepared by the methods of the invention.

A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

The invention belongs to the technical field of building material additive production, and in particular relates to a reagent for inhibiting the shrinkage of cement-based materials. The production method of the reagent comprises the following steps: 1) mixing pomelo peel powder and water into a container to obtain suspension solution, and then add alkaline protease / carboxylate reductase compound enzyme to the container for enzymolysis; 2) after the above enzymolysis is completed, dehydrate and dry to obtain a solid, add a solvent and a catalyst to the solid, and heat it under microwave irradiation Raise the temperature to 85-90°C, heat and irradiate for 20-30min; 3) put the result of step 2) under ultraviolet radiation, add benzophenone and lauryl glucoside, and stir for 10-15min; 4) put the result of step 3) After vacuum stirring the mixture and waste cooking oil for 5-10 minutes, add manganese slag and vacuum stirring to obtain the product.

The invention discloses a method for synthesizing pheromone fatty alcohol acetate in microorganisms based on three intermediate products of fatty acid metabolism pathway as precursor molecules. The reductase CAR, fatty acyl-CoA reductase FAR and aldehyde reductase AHR are used to obtain engineering bacteria that can reduce fatty acyl-ACP, fatty acid or fatty acyl-CoA to produce fatty alcohol, respectively. Finally, alcohol acetyltransferase ATF1 was highly expressed in fatty alcohol-producing engineering bacteria, which was used to catalyze the reaction of fatty alcohol and acetyl-CoA to synthesize pheromone fatty alcohol acetate. The invention can realize the microbial transformation from glucose to fatty alcohol acetate, and provides a feasible way for large-scale biosynthesis of fatty alcohol acetate.