33 results about "Glycerol fermentation" patented technology

This invention provides production of material 1, 3- trimethylene glycol and 2, 3- butylene-glycol by coarse starch. The coarse starch is fermented continuously by yeast cell to obtain the high concentration fermented liquid of glycerine. The fermented liquid of glycerine is filtered to obtain the cells which can be used for the next batch glycerine fermentation. The filtrate is used as the base material for producing 1, 3- trimethylene glycol. Add the nitrogen source in the fermented course. When finishing the fermentation conduct the processes of desalting, distillation, vacuum rectification etc. step to the 3- Trimethylene glycol and 2, 3- Butylene-glycol to obtain the products. At the same time the concention of 1, 3- Trimethylene glycol and 2, 3- Butylene-glycol with high added value is increased through cell back-use and mending material craft. Its advantage lies in: reduced the production cost effectively, raised production efficiency.

The invention relates to a method for producing sophorose ester through fermenting waste molasses and waste glycerin, which comprises the following implementation steps that: (1) spore suspension preparation: torulopsis utilis TJZKBA 10326 is inoculated on the inclined surface of a culture medium and is cultured for 2 to 4 days at the constant temperature being 30 DEG C, and normal saline with the mass concentration being 0.75 percent is added for washing mycelia, and the bacterial suspension is obtained; (2) the bacterial suspension is used for being inoculated into the sterilized liquid seed culture medium, the spore suspension is inoculated into the cooled liquid seed culture medium according to the inoculation quantity with the volume percentage being 2 to 5, and liquid seeds are obtained; (3) the liquid seed culture medium is used for being inoculated into a sterilized 5L fermentation tank, then, the liquid culture medium is inoculated into the cooled fermentation tank culture medium according to the inoculation quantity with the volume percentage being 2 to 5, the fermentation temperature is 30 to 34 DEG C, the dissolved oxygen is controlled to be 30 to 40 percent, and the fermentation time is 48 to 72h; and (4) after the fermentation is completed, the mixed organic solvent of organic solvent ethyl acetate / isopropanol with the same volume as the fermentation liquid is used for extracting the sophorose ester. The method has the advantages that the process is simple, the cost is low, and the effect is obvious.

The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g / L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g / L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity.

The invention discloses a treatment method for biodiesel by-product glycerol. The method includes the following contents: (1) acidifying the biodiesel by-product glycerin, standing for stratification, and taking the lower clear liquid; (2) adding organic alcohol to the lower clear liquid obtained in step (1), mixing evenly, and solidifying After liquid separation, glycerin clear liquid is obtained; (3) The glycerin clear liquid obtained in step (2) is added for dilution, electrodialysis is performed for desalination, and then rectification, and the residue is collected as the product. The glycerin product obtained by the method can be used for microbial fermentation, has a simple process and is easy to realize industrial application.

Glycerol fermentation by candida glycerinogenes NAD+ depends on glycerine-3-phosphate dehydrogenase gene and clone thereof and pertains to the microorganism molecular biology field. The invention relates to a new gene order SEQID NO.1. a degenerate primer PCR method and a reverse PCR method are adopted to clone rate-limiting enzyme NAD+ depending glycerine-3-phosphate dehydrogenase gene and lateral regulation sequence thereof from genome DNA of glycerine-producing candida mycoderma WL2002-5-59 which is produced by glycerine. The total length of the gene is 4900bp and a start codon ATG is at 2082bp while a stop codon TAA is at 3246bp. The sequence has no intron but has an 1167bp complete open reading frame which encodes 388 amino acids. The gene has homology as high as 60 percent with saccharomyces cerevisia GPD1 gene and 70 percent with Angus pichia yeast GPD gene. The clone of the gene expands gene resource of microorganism anti-osmotic pressure stress research and lays a foundation for research on molecular mechanism of glycerine-producing candida mycoderma producing glycerine with high yield.

The invention discloses a genetic engineering strain capable of coproducing isoprene and 1,3-propylene glycol and an establishment method and application thereof, and belongs to the technical field of genetic engineering. In the genetic engineering strain, a glycerol dehydratase gene and a glycerol dehydratase gene reactivating enzyme gene are integrated, a glycerol kinase gene is knocked out, and HMG-CoA reductase, HMG-CoA synthetase, mevalonate kinase, phosphomevalonate kinase, pyrophosphomevalonate decarboxylase, IPP isomerase, isoprene synthetase, aldehyde reductase and transhydrogenase are overexpressed. According to the invention, through genetic engineering, a metabolic way capable of coproducing the isoprene and the 1,3-propylene glycol is successfully established in escherichia coli; by the establishment method, redox cofactors in cells of the genetic engineering strain are metabolized in a balanced way, and the yield in coproducing the isoprene and the 1,3-propylene glycol through glucose and glycerol fermentation is increased.

The invention provides a method for producing 1,3-propanediol by fermentation of glycerol in a microbial co-cultivation system, by successfully constructing recombinant Escherichia coli Rosseta-dhaB1dhaB2 and Rosseta-dhaT, co-cultivating the above-mentioned recombinant Escherichia coli for seed culture and fermentation culture , wherein the recombinant Escherichia coli Rosseta-dhaB1dhaB2 realizes the utilization of glycerol to produce the intermediate metabolite 3-hydroxypropionaldehyde (3-HPA), and the recombinant Escherichia coli Rosseta-dhaT catalyzes 3-HPA to produce 1,3-propanediol. During the fermentation process, press Inoculate in the fermentation medium at a ratio of 1:1.5, add 60g / L substrate glycerol and 10g / L cosubstrate glucose. The method of the present invention improves the conversion rate of glycerol and the output of 1,3-propanediol, and the present invention adopts Clostridium butyricum containing independent coenzyme B 12 Glycerol dehydratase, which reduces the production cost.

The invention discloses a bacterial strain used to produce succinic acid by fermenting crude glycerol, a by-product of biodiesel, and a production method thereof. The bacterial strain Actinobacillus succinoproducens ( Actinobacillus succinogenes ) XT, deposit number CCTCC NO: M2016397, can use biodiesel by-product crude glycerol as raw material to ferment and produce succinic acid, the yield of succinic acid can reach up to 72 g / L, and the yield can reach up to 95%. The production method of the present invention is simple, efficient, and low in cost, realizes the efficient resource utilization of industrial waste crude glycerin, solves the environmental problems caused by the discharge of biodiesel by-product glycerin, extends the biodiesel industry chain, and improves the biodiesel industry. Overall technical level and circular benefits.

The invention discloses a bacterial strain that strengthens glycerol metabolism, and the bacterial strain is classified and named as Actinobacillus succinate ( Actinobacillus succinogenes ) JF1315, which was deposited in China Center for Type Culture Collection on March 31, 2016, and its deposit number is: CCTCC NO: M 2016160. The present invention also relates to the application of the Actinobacillus succinogenes JF1315 in fermentative production of organic acids. Under the anaerobic condition, compared with the control strain, the strain can grow and metabolize at a lower pH condition, and can efficiently utilize glycerol to ferment and synthesize reduced organic acids. Under the condition of pH 4.8-6.8, the strain can grow normally, and metabolize glucose to synthesize organic acids such as succinic acid; in addition, under this condition, the strain can efficiently use glycerol for anaerobic fermentation, synthesize and accumulate organic acids. acid.

The invention relates to the technical field of engineered strain construction, in particular to a construction method of an engineered strain for producing phenol through glycerol fermentation. The second base sequence and the third base sequence in a sequence list are inserted in a pTrc99a carrier to obtain recombinant plasmid; the recombinant plasmid is electrically transformed into an ATC31884 strain, the engineered strain for producing the phenol through glycerol fermentation is obtained. The constructed engineered strain efficiently presents tyrosine phenol lyase and phenylalanine4-monooxygenase, and content of the obtained escherichia coli engineered strain for producing the phenol through glycerol is 21.30-57.66 mg / L, so that the phenol is produced through glycerol fermentation through the biological method, production cost of the phenol is reduced, and economic benefits are improved.

The invention relates to a method for producing 1,3-propanediol by fermentation of glycerin. Firstly, the fermentation strain is cultivated under microaerobic conditions to obtain a fermented seed liquid; then microalgae are cultured under autotrophic conditions to obtain a microalgae seed liquid Then, insert the fermented seed liquid in the fermentation medium to carry out micro-aerobic fermentation, add the microalgae seed liquid when the acetic acid concentration is greater than 3g / L, and cultivate until the end of fermentation; finally adjust the pH to alkaline, and let it stand for stratification A fermentation broth containing 1,3-propanediol is obtained. The method of the invention utilizes the combination of microalgae cell metabolism and fermentation system to strengthen the oxidative phosphorylation at the substrate level in glycerin metabolism and improve the fermentation level.

The invention discloses a method for enhancing osmophilic yeast yeasting glycerine density by adding in amphiprotic zymoid yeasting liquid in glycerine producing field. The invention inoculates somphilic yeast strain in glycerine yeasting incubation media into glycerine yeasting incubation media, at the same time, in the process of glycerine yeasting, adds in amphiprotic zymoid yeasting liquid to carry on glycerine yeasting. The added amphiprotic zymoid yeasting liquid improves the membrane permeability of osmophilic yeast cell, and adjusts the permeability pressure, makes the glycerine in cell secreted out of the cell, thus enhances the density of glycerine to 10-90%. The process is simple, easy to be controlled, low cost, easy to be used in industry.

The invention discloses a production method and application of mannanase. The method for producing mannanase provided by the present invention comprises: introducing the mannanase gene derived from Cladosporium camphoropsis S168 into recipient yeast to obtain recombinant yeast; performing basic fermentation culture, glycerol In the fed-feed fermentation stage and the methanol-feed-induced expression stage, the mannanase was obtained from the fermentation product. The mannanase expression level of the engineering bacteria of the present invention reaches 42200U / mL through high-density fermentation, and the optimal pH of the obtained enzyme is 7.5, and the optimal temperature is 75°C. It has acid and alkali resistance, good thermal stability, and excellent hydrolysis properties. Features, in addition, the present invention broadens the scope of application of mannanase, and the partially hydrolyzed konjac gum prepared by using this enzyme has great application value in industries such as food and feed, enriches the types of prebiotic products, and promotes the development of prebiotic products in my country. The development of yuan industry has great social and economic benefits.

The invention discloses a method for simultaneously preparing 1,3-propylene glycol and 2,3-butylene glycol from glycerol by using microbe employing prior-stage anaerobic and later-stage aerobic two-stage fermentation process, which belongs to the field of the bio-chemical engineering technology. In the fermentation process, the seed liquid is added in the primary fermentation medium containing industrial glycerol or glycerol fermentation liquid, after 3-5 hours, the mixed liquid of glycerol and glucose is added in the form of the flow. The product concentrations of 1,3-propylene glycol and 2,3-butylene glycol are controlled by adjusting the air flow in the two-stage fermentation process and controlling the fermentation temperature. When the fermentation process is over, 1,3-propylene glycol and 2,3-butylene glycol are separated and purified by desalting, distilling, and vacuum rectification. The preparing method can improve the fermentation concentration and the yield of 1,3-propylene glycol and the production intensity, at the same time, 2,3-butylene glycol with higher concentration can be obtained with high raw material availability and low cost.

The invention provides a tea oil hand cream, which is mainly composed of the following components in mass percent: 5-10% of glycerin, 5-10% of mineral oil, 3-6% of caprylic / capric triglyceride, cyclic Pentamethicone 1‑3%, Hydroxyethyl Urea 1‑3%, Cetearyl Alcohol 1‑3%, Cetearyl Glucoside 0.5‑1.5%, Beeswax 0.1‑1%, Liquid Paraffin 0.1‑1%, Cetyl Alcohol 0.1‑1%, Stearyl Alcohol 0.1‑1%, Polyacrylamide 0.05‑0.25%, C13‑14 Isoparaffin 0.05‑0.25%, Laureth‑7 0.05‑ 0.25%, Carbomer 0.1‑0.3%, Xanthan Gum 0.05‑0.15%, Triethanolamine 0.1‑0.3%, Allantoin 0.01‑0.1%, Salicylic Acid 0.01‑0.1%, Phenoxyethanol 0.1‑0.5%, 0.1-0.3% of methylparaben, 0.1-0.2% of ethylparaben, 0.05-0.15% of ethylhexylglycerin, 0.5-5% of fermented camellia oleifera seed oil, 53-80% of water; wherein the fermented camellia oleifera seed oil is composed of It is prepared from the following raw materials: camellia oleifera seed cake, camellia oleifera root, and camellia oleifera. The hand cream of the invention is easy to be absorbed through the skin, and can play a good skin care effect of preventing frost cracking, preventing skin roughness, preventing chapping, moisturizing and rejuvenating the skin, and relieving itching and pain.