32 results about "Sophorose" patented technology

The invention discloses a method for hydrolyzing stevioside with rich sources by using acid and synchronously preparing two products with high added values, namely isosteviol and sophorose. An isosteviol product is a medicine with functions of reducing blood sugar, regulating lipid, and the like, the sophorose is a most effective inducer for producing cellulase from strains such as trichoderma, and the like, and the two products have broad application prospect respectively. The method is characterized in that by controlling appropriate conditions, the two high added value products with important industrial application value and medical value, namely the isosteviol and the sophorose are synchronously prepared, and a one-step hydrolysis method is adopted.

This invention discloses a quasi-Wicker yeast variety Wickerhamiella domercqiae var. sophorolipid. This strain is conserved in general microbe center in China microbe bacterial conservention and management committee (institute of microbe of Chinese Academy of Sciences, Beijing, China), and its preservation number is CGMCC No. 1576. This invention also discloses the appliance of the quasi-Wicker yeast variety in preparing sophorosid biosurfactant that can supress neoplasms outside of body. The sophorosid is prepared through following steps: (1) chamfer culture(2)seed culture(3) enlargement culture(4) fermentate collection(5)production separation, and others. This method has many advantanges, for example, it is easy to operate; the output of sophorosid is high; the generated sophorosid has high restraining rate to tumor cell...so it has very broad application prospect in drug field.

The present invention is directed to the use of rebaudioside A or rebaudioside D in combination with one or more compounds of Formula (I), or a stereoisomer thereof, wherein R2 is rhamnose, and R and R1 are each independently selected from the group consisting of hydrogen, glucose, and beta-sophorose, for enhancing the sweet taste of carbohydrate sweeteners, such as sucrose and fructose. The present invention is also directed to consumables which include a combination of a carbohydrate sweetener, rebaudioside A or rebaudioside D, and one or more compounds of Formula (I), or a stereoisomer thereof.

The invention relates to a beta-glucosidase and an application thereof, wherein the beta-glucosidase comprises: a, enzyme with the sequence of SEQ ID NO:1; and b, enzyme which is derived from the enzyme in step a through substituting, deleting or adding one or a plurality of amino acids and has the activity of the enzyme in the step a. The selected beta-glucosidase provided by the invention has the deoxyglucose and hygromycin resistance and transglycosylation, under the transglycosylation, cellobiose and other oligosaccharides can generate sophorose and other inductors, and enter cells through a constitution permease system on a cell membrane, and the synthesis of cellulose in the cells is initiated. Consequently, the beta-glucosidase can be used for the production of catalytically synthesizing the sophorose to induce the cellulose, and thereby having a greater commercial value.

The present invention is directed to the use of rebaudioside A or rebaudioside D in combination with one or more compounds of Formula (I), or a stereoisomer thereof, wherein R2 is rhamnose, and R and R1 are each independently selected from the group consisting of hydrogen, glucose, and beta-sophorose, for enhancing the sweet taste of carbohydrate sweeteners, such as sucrose and fructose. The present invention is also directed to consumables which include a combination of a carbohydrate sweetener, rebaudioside A or rebaudioside D, and one or more compounds of Formula (I), or a stereoisomer thereof.

The invention discloses a method for producing cellulase by trichoderma reesei fermentation. The method is characterized in that in a trichoderma reesei fermentation culture stage, a mixture of an induction carbon source and a nitrogen source is fed in a fed-batch manner, and preferably, the fed-batch feeding time is 24-26h after fermentation. The induction carbon source is obtained by mixing a biomass material enzymolysis product and a sophorose inducer according to a ratio of (3-5): (7-9), and the nitrogen source is a mixture of corn steep liquor and ammonium sulfate. The method has the advantages that the adding amount of phosphate in a trichoderma reesei fermentation culture medium is optimized, and production of the cellulase by the trichoderma reesei through permentation is performedwith low culture medium cost; the optimal nitrogen source feeding ratio is selected, so that the nitrogen source cost is saved; and the carbon-nitrogen ratio in the feeding process is strictly controlled, so that the trichoderma reesei can synthesize the cellulase at high yield.

The invention relates to a method for producing sophorose ester through fermenting waste molasses and waste glycerin, which comprises the following implementation steps that: (1) spore suspension preparation: torulopsis utilis TJZKBA 10326 is inoculated on the inclined surface of a culture medium and is cultured for 2 to 4 days at the constant temperature being 30 DEG C, and normal saline with the mass concentration being 0.75 percent is added for washing mycelia, and the bacterial suspension is obtained; (2) the bacterial suspension is used for being inoculated into the sterilized liquid seed culture medium, the spore suspension is inoculated into the cooled liquid seed culture medium according to the inoculation quantity with the volume percentage being 2 to 5, and liquid seeds are obtained; (3) the liquid seed culture medium is used for being inoculated into a sterilized 5L fermentation tank, then, the liquid culture medium is inoculated into the cooled fermentation tank culture medium according to the inoculation quantity with the volume percentage being 2 to 5, the fermentation temperature is 30 to 34 DEG C, the dissolved oxygen is controlled to be 30 to 40 percent, and the fermentation time is 48 to 72h; and (4) after the fermentation is completed, the mixed organic solvent of organic solvent ethyl acetate / isopropanol with the same volume as the fermentation liquid is used for extracting the sophorose ester. The method has the advantages that the process is simple, the cost is low, and the effect is obvious.

The invention discloses a method for efficiently producing cellulose based on preparation of a mixture through glucose glucoside conversion reaction. According to the method, glucose or syrup containing glucose is adopted for preparing a sophorose-containing mixture through glucoside conversion reaction under catalysis of beta-glycosidase, the sophorose-containing mixture is adopted as a carbon source and an inductor for culturing Trichoderma reesei, and the cellulose is efficiently produced, wherein in the fermentation tank culture process, through the batch-type feeding of the mixture, the glucose concentration in the fermentation liquor is controlled to be 0.05-0.30 g / L, so that the mycelial growth is facilitated, but the biosynthesis of the cellulose is not inhibited; after the culture is carried out for 144h, the activity of the cellulose in the fermentation liquor exceeds 90.0FPU / mL, and the production intensity of the cellulose in the fermentation tank exceeds 625.0FPU / L / h. Due to the high activity and high production intensity of the cellulose, the investment on the production equipment and the energy consumption during the fermentation process of the cellulose are obviously saved, and the production cost of the cellulose is reduced.

The present invention is directed to the use of a compound of Formula I,or a stereoisomer thereof, wherein R2 is rhamnose, and R and R1 are each independently selected from the group consisting of hydrogen, glucose, and beta-sophorose, for enhancing the sweet taste of carbohydrate sweeteners, such as sucrose and fructose. The present invention is also directed to consumables comprising a combination of a carbohydrate sweetener and a compound of Formula I, or a stereoisomer thereof.

The present invention provides a dried influenza vaccine preparation in which the activity of an influenza vaccine antigen can be stably maintained even when stored without strictly maintaining a low temperature, and which can be stably supplied. The present invention also provides a method of producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation containing an influenza vaccine antigen and a disaccharide, wherein the disaccharide is at least one selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The invention relates to a method for producing cellulase by adding sophorose lipid for inducing fermentation. The method comprises the following implementation steps of: (1) seed culturing: inoculating a cultured trichoderma reesei strain into a sterilized seed culture medium and culturing at the 28-30 DEG C for 68-72 hours; (2) sterilizing a fermentation culture medium: sterilizing the fermentation culture medium at 121 DEG C for 20 minutes till the pH is 5.0, and cooling to 28-30 DEG C; (3) fermentation: transferring a seed liquid into a sterilized 5L fermentation tank with the liquid volume 3L at the inoculum concentration of 5-10 percent, controlling the fermentation temperature at 28-30 DEG C till the pH is 5.0, fermenting for 40 hours and sampling and testing the concentration of the strain every 2 hours; (4) sterilizing sophorose lipid; (5) inducing with the sophorose lipid; and (6) roughly extracting cellulase. According to the method for producing the cellulase by adding the sophorose lipid for inducing fermentation, the synthesis of cellulase is induced effectively, higher cellulase activity is achieved, and the production cost of cellulase is lowered.

The invention relates to a strain for efficiently converting stevioside into rubusoside and a culture method and application thereof. A strain of (Chryseobacterium sp.) 1433 is preserved in the ChinaGeneral Microbiological Culture Collection Center on July 6, 2020, the preservation number is CGMCC No.20188 and the address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Yard, West Beichen road, Chaoyang District, Beijing. The invention also relates to the culture method of the strain and the application of the strain in preparing the rubusoside. The (Chryseobacterium sp.1433) screened and separated by the invention can hydrolyze sophorose of the stevioside into glucosyl to generate the rubusoside and cannot further hydrolyze the rubusoside, so that the conversion rate of the biological strain to the stevioside reaches 100 percent, the yield of the rubusoside reaches 100 percent and the strain is completely superior to the reported related strains.

The present invention concerns a process for the production of sophorose starting from sophorolipid, in which a step for the hydrolysis of sophorolipid is carried out in an aqueous medium in the presence of an acidic compound.

The invention provides a fermentation method for producing high-activity acid cellulase from trichoderma reesei, which comprises the following steps: S1, inoculating a trichoderma reesei strain into a culture medium, separating to obtain a single colony, culturing seeds, and preserving; s2, inoculating the preserved trichoderma reesei into an activated culture medium for culturing; S3, inoculating the culture solution in the step S2 into a seed amplification culture medium for culturing; and S4, inoculating the culture solution in S3 into a fermentation culture medium, and carrying out liquid submerged fermentation to obtain fermentation liquor containing acidic cellulase. According to the method, culture medium components and fermentation conditions are optimized, sophorose is used for inducing enzyme production, trichoderma reesei cellulase production is divided into two stages, the feeding speed in the enrichment period is increased as much as possible so that thalli are rapidly proliferated to 30% of wet weight, after the thalli density is reached, the feeding speed is reduced, the thalli enter the induced enzyme production period, glucose is rapidly consumed, the low-concentration sophorose is transferred into cells to play a strong induction role, so that the high-activity acidic cellulase is obtained in fermentation liquor.

The invention relates to a low-sugar probiotic composition comprising sophorose, xylitol, L-arabinose and dietary fiber. The invention also discloses a preparation method and application of the low-sugar probiotic composition. The low-sugar probiotic composition has the effect of promoting proliferation of intestinal beneficial bacteria or improving metabolic activity, and can induce intestinal microorganisms to secret cellulase and reduce the probability of flatulence.

The purpose of the present invention is to provide the following: a dried influenza vaccine preparation with which stable retention of the activity of influenza vaccine antigen is possible, even when the preparation is stored without strict low-temperature management, and with which a stable supply is possible; and a method for producing the dried influenza vaccine preparation. The present invention provides a dried influenza vaccine preparation comprising influenza vaccine antigen and a disaccharide, wherein the disaccharide is one or more selected from the group consisting of sucrose, maltose, palatinose, melibiose, isomalt, cellobiose, allolactose, isomaltose, sophorose, lactobionic acid, laminaribiose, xylobiose, turanose, gentiobiose, rutinose, kojibiose, nigerose, robinose, neohesperidose, sucralose, and maltitol.

The present invention provides a method to produce RD by using a cutinase to catalyze the esterification of RB under stepwise cooling temperatures, which is related to the field of biosynthesis of organic compounds. The method uses a cutinase from Thermobifida fusca to catalyze the esterification of RB and sophorose to produce RD. The stepwise cooling temperatures are used to reduce the heat inactivation of the enzyme as well as to improve the mass transfer. The method catalyzes the esterification of RB to produce RD in a solvent such as methanol, DMSO and DMF. The reaction is safe, efficient and highly selective. In addition, the method uses stepwise additions of substrate RB and cooling temperatures for the esterification reaction. In this way, it speeds up the initial reaction rate, increases the amount of solved RB as it is converted to RD, and improves the mass transfer to further increase the reaction speed. In summary, the method uses moderate reaction conditions, and has a high yield and a simple purification procedure.

The present invention concerns a method for producing sophorose from a sophorolipid which involves carrying out a step of hydrolysis of the sophorolipid in an aqueous medium in the presence of an acid compound.

The invention relates to a beta-glucosidase and an application thereof, wherein the beta-glucosidase comprises: a, enzyme with the sequence of SEQ ID NO:1; and b, enzyme which is derived from the enzyme in step a through substituting, deleting or adding one or a plurality of amino acids and has the activity of the enzyme in the step a. The selected beta-glucosidase provided by the invention has the deoxyglucose and hygromycin resistance and transglycosylation, under the transglycosylation, cellobiose and other oligosaccharides can generate sophorose and other inductors, and enter cells through a constitution permease system on a cell membrane, and the synthesis of cellulose in the cells is initiated. Consequently, the beta-glucosidase can be used for the production of catalytically synthesizing the sophorose to induce the cellulose, and thereby having a greater commercial value.

The invention discloses toothpaste adopting an oral cavity micro-ecological balance technology and a preparation method thereof, and belongs to the technical field of toothpaste, the toothpaste adopting the oral cavity micro-ecological balance technology comprises the following components in parts by mass: 0.01-2 parts of sophoroester, 0.01-2 parts of rhamnose ester, 0.1-2 parts of sodium phytate, 0.1-0.5 part of zinc citrate and 3-5 parts of beta-cyclodextrin; the sophorose ester and the rhamnose ester both have a lactone type structure, and bacteria cannot be formed by removing a biomass film, so that the growth of the bacteria is inhibited, dental plaque is finally avoided, and dental caries, decayed teeth and dental calculi are prevented.

The invention discloses a method for high-yielding cellulose through trichoderma fungi. The irradiation technology is introduced to modify rice straw, the inducing effect is improved, and the segmentation inducing fermentation technology is adopted. The method specifically comprises the following steps that under the aseptic environment, spores of the trichoderma fungi are inoculated to a PDA plating medium to be cultured, a circle of spores are picked to be inoculated to a seed medium, and seed liquid is obtained after shaking table shake culturing is conducted; the obtained seed liquid is inoculated to a fermentation enzyme producing culture medium containing lactose to be subjected to fermentation cultivation according to a certain inoculation amount; a fresh carbon source containing sophorose is added to a fermentation system through aseptic operation, and fermentation cultivation continues to be conducted; after fermentation is finished, the obtained fermentation liquid is subjected to centrifugal separation, and obtained supernate is the high-yielding cellulose enzyme liquid. A cellulose enzyme system obtained through the method is more perfect in structure and high in yield.

The invention relates to a modified β-glucosidase, its expression gene and application. The modified β-glucosidase amino acid sequence is shown in SEQ ID NO.1; the β-glucosidase expression gene, nucleic acid sequence is shown in SEQ ID NO.2. Utilize β-glucosidase described in the present invention to produce the disaccharides such as laminaribiose, cellobiose, sophorose, compare with existing disaccharide production technology, speed of reaction is obviously quickened, and reaction temperature is gentler, has effectively reduced Production energy consumption and production cost, and the β-glucosidase of the present invention has a high expression level in the recombinant strain, the purification process is simple, and the produced laminaribiose and sophorose content is high, which is suitable for large-scale industrial production.

The present invention relates to the field of microbial production of novel biosurfactants. More specifically, the present invention discloses the usage of a fungal strain such as the yeast Starmerellabombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase for producing high amounts of so-called “symmetrical bolaform sophorosides” where both sophorose moieties are attached through a terminal glycosidic linkage to the hydrophobic linker. In addition, the present invention further discloses that the latter yeast can also be used to produce alkyl sophorosides and symmetrical bolaform glucosides.

The invention provides a low-loss cleaning process for leather and belongs to the field of cleaning. The low-loss cleaning process for leather comprises the following steps: spraying a cleaning liquidto the surface of the leather; erasing the cleaning liquid by using anhydrous lint free cloth; naturally airing the leather; and spraying a leather nursing agent to the surface of the leather. The invention also provides the corresponding cleaning liquid and nursing agent for the process. The cleaning liquid is prepared from sophorose ester, an active polysaccharide-collagen complex, synthesizedhectorite and water. The nursing agent is prepared from an inorganic salt-guar gum complex, water soluble vegetable oil, a chelating agent and water. Residues of harmful substances of the cleaned leather cleaned by the process are reduced, and the leather has a certain mildewproof ability.

The present invention provides a method to produce RD by using a cutinase to catalyze the esterification of RB under stepwise cooling temperatures, which is related to the field of biosynthesis of organic compounds. The method uses a cutinase from Thermobifida fusca to catalyze the esterification of RB and sophorose to produce RD. The stepwise cooling temperatures are used to reduce the heat inactivation of the enzyme as well as to improve the mass transfer. The method catalyzes the esterification of RB to produce RD in a solvent such as methanol, DMSO and DMF. The reaction is safe, efficient and highly selective. In addition, the method uses stepwise additions of substrate RB and cooling temperatures for the esterification reaction. In this way, it speeds up the initial reaction rate, increases the amount of solved RB as it is converted to RD, and improves the mass transfer to further increase the reaction speed. In summary, the method uses moderate reaction conditions, and has a high yield and a simple purification procedure.

The invention discloses an application of sophorose in preparation of products for preventing and treating obesity. The invention further discloses corresponding products of the sophorose. According to the application and the corresponding products, the sophorose is used for lowering appetite and properly stimulating generation of short-chain fatty acids so as to achieve the effect of controllingbody weight; and the regulating and controlling action is relatively moderate and is relatively good in acting effect, and direct acting on a brain, acceptors or enzymes is avoided, so that compared with other drugs, the products do not have side effects, can be eaten as part of daily meals and can also be applied to drugs for preventing and treating obesity patients. The sophorose can also be used for weakening adverse effects such as fasting blood-glucose rising and impaired glucose tolerance dose resulting from high-fat meals.

The invention discloses application of sophorose in products for preventing and treating inflammatory bowel diseases, and further discloses the corresponding products of the sophorose. Research results prove that the sophorose can reduce inflammatory factor levels of inflammatory bowel disease model rats to a certain degree, and thus the sophorose can serve as a drug for treating the inflammatorybowel diseases and can also serve as a part of daily diet to prevent the inflammatory bowel diseases; and the sophorose has mild and good regulation effects, and compared with other drugs, the sophorose has no side effects.

The present invention provides a detergent composition that exhibits excellent storage stability, and especially storage stability at low temperatures, and a detergent composition which can be discharged favorably from a dispenser. This detergent composition contains (a) a fatty acid salt and (b) a sophorose lipid, and is characterized in that: the proportion of component (a) contained in 100 mass% of the detergent composition is 4-18 mass%; relative to a total of 100 parts by mass of component (a), the proportion of fatty acid salts having 12 or fewer carbon atoms is 15 parts by mass or moreand the proportion of fatty acid salts having 16 or more carbon atoms is 60 parts by mass or less; the proportion of acid type sophorose lipid is 80 mass% or more relative to a total of 100 mass% of component (b); and the proportion of component (b) is 5-80 parts by mass relative to a total of 100 parts by mass of component (a).

The invention discloses a method for hydrolyzing stevioside with rich sources by using acid and synchronously preparing two products with high added values, namely isosteviol and sophorose. An isosteviol product is a medicine with functions of reducing blood sugar, regulating lipid, and the like, the sophorose is a most effective inducer for producing cellulase from strains such as trichoderma, and the like, and the two products have broad application prospect respectively. The method is characterized in that by controlling appropriate conditions, the two high added value products with important industrial application value and medical value, namely the isosteviol and the sophorose are synchronously prepared, and a one-step hydrolysis method is adopted.