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A method for producing 1,3-propanediol by fermentation of glycerol

A technology of propylene glycol and glycerol, applied in the field of bioengineering, can solve the problems of high energy consumption, low fermentation level, and many fermentation by-products, etc., and achieves the effects of low energy consumption, prolonging the fermentation period, and reducing the separation process.

Active Publication Date: 2022-03-04
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above methods all started from the facultative anaerobic characteristics of Klebsiella, and achieved certain effects through stage regulation and strain transformation, etc., it was found from the research on the mechanism of metabolic pathways: (1) Glycerol metabolism under anaerobic conditions The selectivity is the highest, but a high-flux nitrogen gas is required to maintain micro-aerobic conditions during the process, and the energy consumption is high; (2) Under micro-aerobic and aerobic conditions, there are more by-products of fermentation, and genetically engineered bacteria are mostly used at present. But the fermentation level is not high

Method used

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  • A method for producing 1,3-propanediol by fermentation of glycerol
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  • A method for producing 1,3-propanediol by fermentation of glycerol

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Experimental program
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Effect test

Embodiment 1

[0042] (1) Strain cultivation: Take 70mL of activated Klebsiella bacteria liquid, mix it with 630mL seed medium in a 1L fermenter, and carry out micro-aerobic culture with a ventilation rate of 0.02vvm; the culture conditions are: the culture temperature is 37°C, The stirring speed was 200 rpm, the pH was controlled at 7.0, and the fermented seed liquid was obtained after culturing for 18 hours.

[0043] (2) Microalgae culture: Mix 60mL of algae liquid of cellulosic algae SS-B7 in the logarithmic growth phase and 540mL of microalgae medium in a column reactor (40mm in inner diameter, 500mm in height), and CO2 is introduced during the cultivation process. 2 Air mixture with a content of 3v%, light intensity of 4000Lux, culture temperature of 28°C, pH value of 7.5, light cycle of 24h, light-to-dark time ratio of 14:10, cultivated until the algae cells in the microalgae seed liquid are dry. The weight reaches 18.2g / L.

[0044] (3) Fermentation process: The volume of the fermente...

Embodiment 2

[0048] (1) Strain cultivation: Take 70mL of activated Klebsiella bacteria liquid, mix it with 630mL seed culture medium in a 1L fermenter, and carry out micro-aerobic culture with an air intake of 0.06vvm; the culture conditions are: the culture temperature is 37 °C, the stirring speed is 300 rpm, the pH is controlled at 7.2, and the fermented seed liquid is obtained after culturing for 18 hours.

[0049] (2) Microalgae cultivation: Mix 30mL of algae liquid of cellulosic algae SS-B7 in the logarithmic growth phase and 570mL of medium in a column reactor (inner diameter 40mm, height 500mm), and CO2 is introduced during the cultivation process. 2 The content of air mixture is 10v%, the light intensity is 7000Lux, the culture temperature is 37°C, the pH value is controlled at 7.5, the light cycle is 24h, and the light-dark time ratio is 16:8. The weight reaches 19.6g / L.

[0050] (3) Fermentation process: The volume of the fermenter is 15L, and the liquid volume is 7L.

[0051] ...

Embodiment 3

[0054] The fermentation process and process conditions are the same as in Example 1. The difference is that the pH is adjusted to 7.5 after the microalgae seed solution is added during the fermentation process.

[0055] After 40 hours of fermentation, the detected 1,3-propanediol concentration was 78.2g / L, the acetic acid concentration was 4.6g / L, and the conductivity was 16.8mS / cm. End the fermentation, adjust the pH to 8.5, let it stand for 8 hours, the fermentation broth is separated, and the supernatant is OD 600 、OD 280 The detection values ​​were 1.0 and 10.6, respectively.

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Abstract

The invention relates to a method for producing 1,3-propanediol by fermentation of glycerin. Firstly, the fermentation strain is cultivated under microaerobic conditions to obtain a fermented seed liquid; then microalgae are cultured under autotrophic conditions to obtain a microalgae seed liquid Then, insert the fermented seed liquid in the fermentation medium to carry out micro-aerobic fermentation, add the microalgae seed liquid when the acetic acid concentration is greater than 3g / L, and cultivate until the end of fermentation; finally adjust the pH to alkaline, and let it stand for stratification A fermentation broth containing 1,3-propanediol is obtained. The method of the invention utilizes the combination of microalgae cell metabolism and fermentation system to strengthen the oxidative phosphorylation at the substrate level in glycerin metabolism and improve the fermentation level.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for producing 1,3-propanediol by fermentation of glycerin. Background technique [0002] 1,3-Propanediol (1,3-Propanediol, PDO) is mainly used in the production of new polyester PTT (Polytrimethylene Terephthalate) with excellent performance. The polyester synthesized from it has unique properties and excellent performance, and can make polyester plastics biodegradable for easy natural circulation. In recent years, as an important organic synthesis raw material and intermediate, it has become a research and development hotspot because of its unique properties and wide range of uses. [0003] The industrial production methods of 1,3-propanediol mainly fall into two categories: chemical method and biological method. Biological method is the focus of current research, and can be divided into one-step glucose conversion method and glycerin conversion met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/18C12R1/22C12R1/89C12R1/01
CPCC12P39/00C12P7/18
Inventor 张霖樊亚超师文静
Owner CHINA PETROLEUM & CHEM CORP
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