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Method for mixed culture of R.salina

A technology of Haematocystis salina and mixed culture, applied in microorganism-based methods, biochemical equipment and methods, unicellular algae, etc. The effect of reducing the cost of pretreatment

Active Publication Date: 2017-07-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The source of soybeans mainly has many problems such as low relative SA content and geographical restrictions, which lead to bad smell and high purification cost.

Method used

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  • Method for mixed culture of R.salina
  • Method for mixed culture of R.salina
  • Method for mixed culture of R.salina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Halobacter salina (R.salina) seed medium:

[0033] Salina derived seawater, glycerin 10%, Na 2 EDTA·2H 2 O 80mM, H 3 BO 3 2mM, FeCl 3 ·6H 2 O5.02mM, MnSO 4 ·H 2 O 0.01mM, ZnSO 4 ·7H 2 O 0.001mM, CoCl 2 ·6H 2 O 0.001mM, Na 2 EDTA·2H 2 O50mM, HCl 0.1M, HEPES buffer pH 7.8 10mM, Vitamin B 12 0.3g / L.

[0034] The seeds were cultured for two generations, and each generation was carried out in a Erlenmeyer flask with a liquid volume of 50 mL / 250 mL, with an inoculum size of 20% (V / V), and cultured on a shaking table at 23° C. and 120 rpm for 48 hours.

[0035] Then the seed culture medium and the glycerol fermentation medium were respectively according to 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1: 1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9 and 1:10 were mixed, and Haematobacter salina was mixed with 20% (V / V) of the inoculum was sequentially inserted into the mixed medium with a ratio of 10:1 between the seed medium and the glycerol fermentation medium...

Embodiment 2

[0048] Halobacter salina (R.salina) seed medium:

[0049] Salina derived seawater, glycerin 10%, Na 2 EDTA·2H 2 O 80mM, H 3 BO 3 2mM, FeCl 3 ·6H 2 O5.02mM, MnSO 4 ·H 2 O 0.01mM, ZnSO 4 ·7H 2 O 0.001mM, CoCl 2 ·6H 2 O 0.001mM, Na 2 EDTA·2H 2 O50mM, HCl 0.1M, HEPES buffer pH 7.8 10mM, Vitamin B 12 0.3g / L.

[0050] The seeds were cultured for two generations, and each generation was carried out in a Erlenmeyer flask with a liquid volume of 50 mL / 250 mL, with an inoculum size of 20% (V / V), and cultured on a shaking table at 23° C. and 120 rpm for 48 hours.

[0051] Then the seed culture medium and the glycerol fermentation medium were respectively according to 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1: 1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9 and 1:10 were mixed, and Haematobacter salina was mixed with 20% (V / V) of the inoculum was sequentially inserted into the mixed culture medium with a ratio of 10:1 between the seed culture medium and the glycerol fer...

Embodiment 3

[0064] Halobacter salina (R.salina) seed medium:

[0065] Salina derived seawater, glycerin 10%, Na 2 EDTA·2H 2 O 80mM, H 3 BO 3 2mM, FeCl 3 ·6H 2 O5.02mM, MnSO 4 ·H 2 O 0.01mM, ZnSO 4 ·7H 2 O 0.001mM, CoCl 2 ·6H 2 O 0.001mM, Na 2 EDTA·2H 2 O50mM, HCl 0.1M, HEPES buffer pH 7.8 10mM, Vitamin B 12 0.3g / L.

[0066] The seeds were cultured for two generations, and each generation was carried out in a Erlenmeyer flask with a liquid volume of 50 mL / 250 mL, with an inoculum size of 20% (V / V), and cultured on a shaking table at 23° C. and 120 rpm for 48 hours.

[0067] Then the seed culture medium and the glycerol fermentation medium were respectively according to 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1: 1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9 and 1:10 were mixed, and Haematobacter salina was mixed with 20% (V / V) of the inoculum was sequentially inserted into the mixed medium with a ratio of 10:1 of the seed medium and the glycerol fermentation medium, and...

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Abstract

The invention provides a method for mixed culture of R.salina; the method includes the steps: culturing R.salina with a seed culture medium; carrying out domestication culture by using glycerol; inoculating a glycerol fermentation culture medium with the R.salina after domestication; and culturing the R.salina by using light with different wavelengths and intensities. The thallus substrate utilization rate, growth rate, living weight, and phycoerythrin, total protein, polyunsaturated fatty acid and total lipid contents during R.salina fermentation are improved, the problems of harvest of the R.salina are reduced, the biomass concentration and the pre-processing cost of target product extraction are reduced.

Description

technical field [0001] The invention belongs to the technical field of microbe application, and in particular relates to a method for mixed culturing Haematospora salina. Background technique [0002] Stearidonic acid (18:4n-3) (SA) is an important ω-3 series polyunsaturated fatty acid (Δ6,9,12,15-all-cis-stearatetraenoic acid), as The precursor of EPA, the conversion efficiency of SA in animals is much higher than that of arachidonic acid. It can help reduce the content of cholesterol and triglycerides in the body, and promote the metabolism of saturated fatty acids in the body, thereby reducing blood viscosity and improving blood circulation. Circulation, improve tissue oxygen supply and eliminate fatigue. Prevent the deposition of fat on the blood vessel wall, prevent the formation and development of atherosclerosis, prevent cerebral thrombosis, cerebral hemorrhage, hypertension and other cardiovascular diseases. At present, SA mainly comes from vegetable oil (high SA s...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 常明刘睿杰王兴国金青哲
Owner JIANGNAN UNIV
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