The invention discloses a method for
enzymatic synthesis of
theaflavin TF by virtue of tea
polyphenol oxidase isoenzyme PPO1. The method is the optimization of a technique and a technology, which is capable of achieving the oriented
enzymatic synthesis of a single
theaflavin component TF through an enzymatic reaction with a shaking table as well as subsequent steps of extracting, centrifuging, concentrating, freeze-
drying and the like by taking tea
polyphenol oxidase isoenzyme PPO1 as an
enzyme source, catechins EC and EGC components in tea
polyphenol a major reaction substrates and a
buffer solution at a certain pH value as a
reaction system. The method overcomes shortcomings that multiple
theaflavin components are generated simultaneously, a technology for separating and purifying the theaflavin components is complex, the preparation of a high-purity theaflavin component is quite difficult and the like in a conventional process which carries out the
enzymatic synthesis of theaflavin by virtue of crude
enzyme fluid of tea
polyphenol oxidase; the method achieves a technological innovation that a
single product is generated from enzymatic synthesis and a
side product is avoided at reaction end, and the method avoids a technological
bottleneck in subsequent separation and purification of the theaflavin component, so as to lay the theoretical and practical foundation for the green, safe and efficient large-scale industrial production and utilization of the theaflavin component.