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A method and application of directly using non-detoxified acid to pretreat lignocellulose for hydrogen production

A lignocellulose and acid pretreatment technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of reducing production costs, changing metabolic pathways, and good applicability

Inactive Publication Date: 2021-07-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expected cost of the process is low and the treatment cost is also low. The fermentation process is simple and the hydrogen output is high. At the same time, the environmental pollution caused by pretreatment is reduced, and the cost of raw materials and pretreatment is greatly saved. The utilization rate of the substrate solves the problem of low efficiency of lignocellulose in the biohydrogen production process, and has high economic feasibility

Method used

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  • A method and application of directly using non-detoxified acid to pretreat lignocellulose for hydrogen production
  • A method and application of directly using non-detoxified acid to pretreat lignocellulose for hydrogen production
  • A method and application of directly using non-detoxified acid to pretreat lignocellulose for hydrogen production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Hydrogen production capacity of different concentration substrates in synchronous saccharification

[0069] (1) Preparation of a seed fluid of thermophilic fluid oires: The thermophilic polytenee anaerobacterium MJ1 preserved -80 ° C is first passed through 10 ml of Willin bottle (with 4.5 mL seed medium) at 55 ° C, 150 rpm oscillating culture. 18h was activated, followed by amplification at 100 mL of serum bottle (45 mL seed medium) at 55 ° C, 150 rpm oscillated culture for 18 h, resulting in seed fluid. The seed medium is a xylose liquid medium containing 5 g / L xylose. The main components of the medium are: xylose 5g / L (carbon source), 1 g / L of ammonium chloride, 1 g / L of sodium chloride, hydrogen phosphate 1 g / L of potassium, 1 g / L of phosphate, 0.5 g / L, hexamine chloride 0.5 g / L, potassium chloride 0.2g / L, yeast powder 2g / L, protein 胨 2g / L, Trual element reservoir 1 ml / L, 1ml / L of vitamin reservoir, 0.01% blade Tianqing 1ml / L;

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Embodiment 2

[0077] Example 2: Hydrogen production capacity of different concentration substrates in conjunctive synchronous saccharification

[0078] (1) Preparation of seed fluid as in Example 1.

[0079] (2) Preparation of fermentation medium and sterilization as in Example 1.

[0080] (3) The first stage in the preheating synchronous aerobacterial prehydration synchronous saccharide fermentation mode is hydrogenated: the cellulase CTEC2 is injected into the medium with a syringe of 20U / g canesets, and after 50 ° C is enzymatically dissociated after 72 h. Further, the prepared seed liquid was injected into the fermentation medium after 10% (V / V) inoculation, and the fermentation temperature was 55 ° C, the shaker was 150 rpm, and the fermentation was terminated after fermentation 120h. Such as figure 2 As shown, the yield of hydrogen is increased as the substrate concentration increases, and the output is 20.71 mm (20g / L sugar canesets), 57.78 mm (40 g / L sugar cane), and 76.78 mm (6...

Embodiment 3

[0081] Example 3: Method for carrying hydrogen production in different fermentation modes

[0082] (1) Preparation of seed fluid as in Example 1.

[0083] (2) Preparation of fermentation medium and sterilization as in Example 1.

[0084] (3) The first stage of hydrogenation in synchronous saccharification fermentation mode in synchronous saccharification is in Example 1.

[0085] (4) EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc.

[0086] (5) Second stage fermentation hydrogen in different fermentation modes: After the end of the fermentation, synchronous glycained fermentation and prehydrolysis synchronous saccharification fermentation of the fermentation medium (100 g / L sugarcane slag) is adjusted to 7.0, The serum bottle was sealed with a glue and aluminum cover and repeatedly pumped with 0.01 MPa of nitrogen 3 times, transferred to the purity of the second stage, the fermentation temperature was 55 ° C, the shaker was 150 rpm, ferment 120h After the end of the fermentation. Such as im...

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Abstract

The invention discloses a method and application of hydrogen production by directly using non-detoxified acid to pretreat lignocellulose. The invention inoculates the thermophilic saccharolytic anaerobic bacillus seed liquid into the fermentation medium to carry out anaerobic fermentation to obtain hydrogen; wherein, the fermentation medium is a medium in which undetoxified acid pretreated lignocellulose is used as a carbon source. The invention carries out fermentation through two-stage fermentation or adding some organic acids. The present invention can reach 277.44mM (6.21L-H) through the output of two-stage fermentation hydrogen 2 / L); by adding 10g / L acetic acid, the production of hydrogen can be increased by 63.9%, which can significantly improve the biohydrogen production efficiency of bagasse. The method provided by the invention is suitable for transforming bagasse by microorganisms.

Description

Technical field [0001] The present invention belongs to the field of microbial fermentation hydrogen production technology, and in particular, the method and application of directly producing hydrogen cellulose use with unreserted acid pretreatment of lignocellulose. Background technique [0002] As the human beings entered the 21st century, the development of various techniques, leading to a large amount of fossil fuel, such as coal, oil and gas, etc., which provided 95% of the current demand for the world. With the further development of society, the demand for energy will be further expanded. A large amount of consumption of fossil fuels also causes serious environmental pollution problems, such as the current haze problem, leading to people to talk. More importantly, the non-reproducibility of fossil fuels, the exhaustion of energy will affect the stability of society, find a renewable, environmentally friendly and low cost alternative energy source being concerned by countri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/01
CPCC12P3/00C12P2201/00
Inventor 朱明军胡彬彬
Owner SOUTH CHINA UNIV OF TECH
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