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Method for producing phycoerythrin and polyunsaturated fatty acid by using R. salina

A technology of unsaturated fatty acids and Haematospora salina, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of low relative content of SA, high purification cost, bad smell, etc. Achieve the effect of reducing the cost of pre-treatment

Active Publication Date: 2017-05-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The source of soybeans mainly has many problems such as low relative SA content and geographical restrictions, which lead to bad smell and high purification cost.

Method used

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  • Method for producing phycoerythrin and polyunsaturated fatty acid by using R. salina
  • Method for producing phycoerythrin and polyunsaturated fatty acid by using R. salina
  • Method for producing phycoerythrin and polyunsaturated fatty acid by using R. salina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Halobacter salina (R.salina) seed medium:

[0033] Salina derived seawater, glycerin 10%, Na 2 EDTA·2H 2 O 80mM, H 3 BO 3 2mM, FeCl 3 ·6H 2 O5.02mM, MnSO 4 ·H 2 O 0.01mM, ZnSO 4 ·7H 2 O 0.001mM, CoCl 2 ·6H 2 O 0.001mM, Na 2 EDTA·2H 2 O50mM, HCl 0.1M, HEPES buffer pH 7.8 10mM, Vitamin B 12 0.3g / L.

[0034] The seeds were cultured for two generations, and each generation was carried out in a Erlenmeyer flask with a liquid volume of 50 mL / 250 mL, with an inoculum size of 20% (V / V), and cultured on a shaking table at 23° C. and 120 rpm for 48 hours. The second-generation seeds are inserted into a 400mL / 600mL explosion-type reactor with a 20% (V / V) inoculation amount. The reactor is an artificial seawater fermentation medium, and the white light intensity is 50-350 μmol m -2 the s -1 , the fermentation condition is 23℃, the aeration rate is 0.1m 3 min, fermented for 10 days.

[0035] The composition of the artificial seawater fermentation medium is, NaCl ...

Embodiment 2

[0046] Halobacter salina (R.salina) seed medium:

[0047] Salina derived seawater, glycerin 10%, Na 2 EDTA·2H 2 O 80mM, H 3 BO 3 2mM, FeCl 3 ·6H 2 O5.02mM, MnSO 4 ·H 2 O 0.01mM, ZnSO 4 ·7H 2 O 0.001mM, CoCl 2 ·6H 2 O 0.001mM, Na 2 EDTA·2H 2 O50mM, HCl 0.1M, HEPES buffer pH 7.8 10mM, Vitamin B 12 0.3g / L.

[0048] The seeds were cultured for two generations, and each generation was carried out in a Erlenmeyer flask with a liquid volume of 50 mL / 250 mL, with an inoculum size of 20% (V / V), and cultured on a shaking table at 23° C. and 120 rpm for 48 hours. The second-generation seeds are inserted into a 400mL / 600mL explosion reactor with a 20% (V / V) inoculation amount. The reactor is an artificial seawater fermentation medium, and the red light intensity is 50-350 μmol m -2 the s -1 , the fermentation condition is 23℃, the aeration rate is 0.1m 3 min -1 , fermented for 10 days. Among them, the wavelength of the red light is 590-670 nm, and the wave peak is 640...

Embodiment 3

[0060] Halobacter salina (R.salina) seed medium:

[0061] Salina derived seawater, glycerin 10%, Na 2 EDTA·2H 2 O 80mM, H 3 BO 3 2mM, FeCl 3 ·6H 2 O5.02mM, MnSO 4 ·H 2 O 0.01mM, ZnSO 4 ·7H 2 O 0.001mM, CoCl 2 ·6H 2 O 0.001mM, Na 2 EDTA·2H 2 O50mM, HCl 0.1M, HEPES buffer pH 7.8 10mM, Vitamin B 12 0.3g / L.

[0062] The seeds were cultured for two generations, and each generation was carried out in a Erlenmeyer flask with a liquid volume of 50 mL / 250 mL, with an inoculum size of 20% (V / V), and cultured on a shaking table at 23° C. and 120 rpm for 48 hours. The second-generation seeds are inserted into a 400mL / 600mL explosion-type reactor with a 20% (V / V) inoculation amount. The reactor is an artificial seawater fermentation medium, and the blue light intensity is 50-350 μmol m -2 the s -1 , the fermentation condition is 23℃, the aeration rate is 0.1m 3 min -1 , fermented for 10 days. Among them, the wavelength of the blue light is 420-510 nm, and the wave peak...

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Abstract

The invention provides a method for producing phycoerythrin and polyunsaturated fatty acid by using R. salina. The method comprises the steps of culturing the R. salina by using a seed culture medium; inoculating an artificial seawater fermentation medium with the R. salina; culturing the R. salina by using light with different wave lengths and intensities. The method for producing the phycoerythrin and the polyunsaturated fatty acid by using the R. salina increases the substrate utilization rate, and improves the biomass as well as the fermentation level of total protein, the phycoerythrin, lipid and the polyunsaturated fatty acid, thus being very beneficial to promotion of industrial development of producing the phycoerythrin and the polyunsaturated fatty acid by fermenting the R. salina.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a method for producing phycoerythrin and polyunsaturated fatty acids by using Haematobacillus salina. Background technique [0002] Stearidonic acid (18:4n-3) (SA) is an important ω-3 series polyunsaturated fatty acid (Δ6,9,12,15-all-cis-stearatetraenoic acid), as The precursor of EPA, the conversion efficiency of SA in animals is much higher than that of arachidonic acid. It can help reduce the content of cholesterol and triglycerides in the body, and promote the metabolism of saturated fatty acids in the body, thereby reducing blood viscosity and improving blood circulation. Circulation, improve tissue oxygen supply and eliminate fatigue. Prevent the deposition of fat on the blood vessel wall, prevent the formation and development of atherosclerosis, prevent cerebral thrombosis, cerebral hemorrhage, hypertension and other cardiovascular diseases. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12P7/64C12R1/89
CPCC07K14/405C12N1/12C12P7/6427
Inventor 常明刘睿杰王兴国金青哲
Owner JIANGNAN UNIV
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