Production method of butyl alcohol by fermentation of biomass coupled with pervaporation separation

A biomass fermentation and pervaporation technology, which is applied in the field of biomass fermentation coupled with pervaporation separation and production of butanol, can solve problems such as high energy consumption, improve separation efficiency, improve fermentation yield and substrate utilization, and reduce inhibition effect of action

Active Publication Date: 2014-07-02
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this patent, since the total solvent concentration in the side feed liquid of pervaporation is only 7-40%, the energy consumption of the rectification process is still relatively large

Method used

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  • Production method of butyl alcohol by fermentation of biomass coupled with pervaporation separation
  • Production method of butyl alcohol by fermentation of biomass coupled with pervaporation separation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. The fermentation substrate undergoes continuous fed-batch biomass fermentation to obtain butanol fermentation broth

[0049] In this process, continuous fed-batch anaerobic fermentation is carried out with glucose as the fermentation substrate to obtain butanol fermentation broth. The process flow diagram is shown in figure 1 .

[0050] (1) Prepare culture medium

[0051] Prepare a synthetic medium with glucose as the fermentation substrate. The medium consists of glucose 60g / L, ammonium acetate 2.2g / L, potassium dihydrogen phosphate 1g / L, dipotassium hydrogen phosphate 1g / L, magnesium sulfate 0.3g / L and Other trace elements and growth factors. The prepared synthetic medium was transferred into the fermenter 1 for high-temperature sterilization, and after the temperature in the fermenter 1 dropped to 40° C., sterile nitrogen was introduced to create an oxygen-free environment.

[0052] (2) Vaccination

[0053] Inject the activated strain Clostridium acetobutylicu...

Embodiment 2

[0068] 1. The fermentation substrate is subjected to batch biomass fermentation to obtain butanol fermentation liquid

[0069] In this process, the butanol fermentation broth is obtained by intermittent anaerobic fermentation using sugars in the sweet sorghum slag hydrolyzate as a carbon source. The schematic diagram of the process is shown in figure 2 .

[0070] (1) Prepare culture medium

[0071] The medium is prepared with sweet sorghum slag hydrolyzate as the substrate, and the medium is composed of total sugar 50g / L, potassium dihydrogen phosphate 0.9g / L, dipotassium hydrogen phosphate 0.9g / L, magnesium sulfate 0.25g / L and other trace amounts elements and growth factors. The prepared medium was transferred into the fermenter 1 for high-temperature sterilization, and after the temperature in the fermenter 1 dropped to 40° C., sterile nitrogen was introduced to create an oxygen-free environment.

[0072] (2) Vaccination

[0073] Inject the activated strain Clostridium ...

Embodiment 3

[0087] 1. The fermentation substrate is fed-batch biological fermentation to obtain butanol fermentation liquid

[0088] In this process, the butanol fermentation liquid is obtained by fed-batch anaerobic fermentation with the carbohydrates in the sap of sweet sorghum stalk as the carbon source. The process flow diagram is shown in figure 2 .

[0089] (1) Prepare culture medium

[0090] Prepare the medium with sweet sorghum stalk juice as the substrate, the medium composition is 65g / L of total sugar, 0.8g / L of potassium dihydrogen phosphate, 0.8g / L of dipotassium hydrogen phosphate, 0.25g / L of magnesium sulfate and other trace amounts elements and growth factors. The prepared medium was transferred into the fermenter 1 for high-temperature sterilization, and after the temperature in the fermenter 1 dropped to 40° C., sterile nitrogen was introduced to create an oxygen-free environment.

[0091] (2) Vaccination

[0092] Inject the activated strain Clostridium acetobutylicu...

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Abstract

The invention relates to a production method of butyl alcohol by fermentation of biomass coupled with pervaporation separation. The production method comprises the following steps of: firstly, by utilizing the characteristic that an oil-water layering phenomenon of a penetration side liquid occurs after biomass fermentation liquor passes through an ethanol-permselective pervaporation membrane system, respectively carrying out high concentration of product butyl alcohol on water phase and oil phase at the penetration side by selecting the ethanol-permselective pervaporation membrane system and a water-permselective pervaporation membrane system to obtain high-concentration organic concentrate with few moisture content, wherein the concentration of the obtained organic concentrate can reach 99.5wt%, so that the separation efficiency is greatly improved and the energy consumption in the production process is reduced; and secondly, the production method couples the pervaporation technology with the biomass fermentation process so as to realize the timely separation and removal of butyl alcohol in the fermentation medium, reduce the inhibiting effect of butyl alcohol on microbial cell growth, improve the fermentation production rate and substrate utilization rate and greatly reduce the production cost.

Description

technical field [0001] The invention belongs to the technical field of separation of metabolites in a biomass fermentation process, and relates to a method for producing butanol through separation of biomass fermentation coupled with pervaporation. Background technique [0002] The rapid development of the global economy has led to an increasing demand for energy in various countries. The contradiction is that the reserves of fossil energy are limited and depleted, and a large amount of harmful gases are produced during combustion, which pollutes the environment. Therefore, the development of renewable and clean energy The research has aroused extensive attention and attention. [0003] As a second-generation biofuel with great potential, butanol is mainly produced by fermentation (ABE fermentation) from corn, molasses, and agricultural waste rich in cellulose and hemicellulose. Compared with fossil fuels, butanol has The production raw materials come from a wide range of s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C07C31/12C07C29/78C12R1/145
Inventor 秦培勇李树峰谭天伟秦帆
Owner BEIJING UNIV OF CHEM TECH
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