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Method for producing ethanol by adopting mixed culture organism by means of glycerol fermentation

A technology of mixed culture and microorganisms, applied in the field of fermentation, can solve problems such as unfavorable growth of microorganisms

Inactive Publication Date: 2012-10-03
TAICANG ZHOUSHI CHEM PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glycerol is used as a raw material for fermentation. Although glycerol can be used as a carbon source in the medium, high concentrations of glycerol are very unfavorable to the growth of microorganisms. To increase the production of ethanol, this problem must be solved

Method used

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  • Method for producing ethanol by adopting mixed culture organism by means of glycerol fermentation
  • Method for producing ethanol by adopting mixed culture organism by means of glycerol fermentation
  • Method for producing ethanol by adopting mixed culture organism by means of glycerol fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This implementation case illustrates the impact of different initial concentrations of glycerol on ethanol production. Fill 400 mL of medium in a 1 L Erlenmeyer flask, the medium is peptone 2, yeast powder 2, magnesium sulfate 1.5, dipotassium hydrogen phosphate 5, dihydrogen phosphate Potassium 5, ammonium sulfate 1, pH 4.5, and glycerin concentrations were 20 g / L, 40 g / L, 60 g / L, and 80 g / L, respectively. Put the prepared red yeast seed liquid into the fermentation medium with an inoculation amount of 5%, and culture it with shaking at 32°C and 180 rpm / min for 48 h; inoculate the prepared Bacillus macerans into the red yeast rice according to the inoculum amount of 10% In the fermentation broth, the culture was static at 41°C; 100 mL of fresh red yeast rice broth containing 20 g / L glycerol was added every 60 h, and fermented for 300 h. After the fermentation, the ethanol concentration in the fermentation broth was detected by gas chromatography. get results like fig...

Embodiment 2

[0027] This implementation case illustrates the influence of different glycerol concentrations in feed on ethanol production. Fill 400 mL of medium in a 1 L Erlenmeyer flask, the medium is glycerol 20, peptone 2, yeast powder 2, magnesium sulfate 1.5, dipotassium hydrogen phosphate 5. Potassium dihydrogen phosphate 5, ammonium sulfate 1, pH 4.5. Put the prepared red yeast seed liquid into the fermentation medium with an inoculation amount of 5%, and culture it with shaking at 32°C and 180 rpm / min for 48 h; inoculate the prepared Bacillus macerans into the red yeast rice according to the inoculum amount of 10% The fermentation broth was cultured at 41°C; 100 mL of fresh red yeast broth containing 20 g / L, 40 g / L, 60 g / L, and 80 g / L glycerol was added every 60 h, and fermented for 300 h. After the fermentation, the ethanol concentration in the fermentation broth was detected by gas chromatography. get results like figure 2 shown.

[0028] Have figure 2 It can be seen that d...

Embodiment 3

[0030] This implementation case illustrates the influence of different feeding time intervals on ethanol production in feeding. Put 400 mL of medium in a 1 L Erlenmeyer flask. The medium is glycerol 20, peptone 2, yeast powder 2, magnesium sulfate 1.5, hydrogen phosphate Dipotassium 5, potassium dihydrogen phosphate 5, ammonium sulfate 1, pH 4.5. Put the prepared red yeast seed liquid into the fermentation medium with an inoculation amount of 5%, and culture it with shaking at 32°C and 180 rpm / min for 48 h; inoculate the prepared Bacillus macerans into the red yeast rice according to the inoculum amount of 10% In the fermentation broth, culture at 41°C; add 100 mL fresh red yeast rice broth containing 20 g / L glycerol at regular intervals, the time intervals are 30 h, 40 h, 50 h, 60 h, 70 h, and ferment 300 h. After the fermentation, the ethanol concentration in the fermentation broth was detected by gas chromatography. get results like image 3 shown.

[0031] Depend on ...

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Abstract

The invention provides a method for producing ethanol by adopting paenibacillus macerans and monasucs by means of glycerol mixed culture fermentation. According to the invention, the paenibacillus macerans and monasucs are taken as original strains and glycerol as raw materials, researches are carried out on initial glycerol concentration, glycerol concentration in feeding supplement, feeding supplement time interval, fermentation time and slurry quantity that influence the ethanol yield.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for producing ethanol by fermenting glycerin with mixed culture microorganisms. Background technique [0002] At present, petrochemical energy is increasingly exhausted, and people are devoting themselves to the development of various new energy sources, among which bioenergy is favored by people, among which ethanol and biodiesel are two prominent bioenergy sources. The fermentation production of ethanol generally uses starchy raw materials, which consume a large amount of grains and compete with the people for food; in addition, ethanol fermentation can also use crop straws as raw materials. Although the raw materials are sufficient, the hydrolysis efficiency of cellulase is low, resulting in production costs. too high. [0003] Biodiesel is currently a highly valued renewable bioenergy product. It is mainly produced through transesterification. Durin...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/06C12P7/08C12R1/07C12R1/645
CPCY02E50/17Y02E50/10
Inventor 周雪琴
Owner TAICANG ZHOUSHI CHEM PROD
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