Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing phytase taking glycerol as carbon source

A technology of phytase and glycerol, applied in the field of preparing phytase, can solve the problems of unfavorable yeast growth, increase production process and cost, etc., and achieve the effect of reducing processing cost, production cost and cost.

Inactive Publication Date: 2008-05-21
LIAONING HUAXING BIOTECHOLOGY
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are: first, the caramelization reaction produces harmful substances, which is not conducive to the growth of yeast; second, the post-processing of the dark fermented liquid requires decolorization, which increases the production process and cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing phytase taking glycerol as carbon source
  • Method for preparing phytase taking glycerol as carbon source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] A method for preparing phytase using glycerol as a carbon source, adopting the fermentation production process of phytase-producing genetically engineered bacteria using Pichia pastoris as a carrier, is characterized in that glycerol is used as a carbon source, and glycerol as a carbon source is The fermentation stage is added in two steps: the first step is added with the basic fermentation medium, and the second step is added with 50% glycerin solution in the production fermentation.

[0010] The specific process is as follows:

[0011] 1. Culture medium

[0012] 1. Seed medium: 0.5-5% glycerin, 0.5-5% peptone, 0.1-2% yeast powder, according to the percentage of dissolution, and the rest is water;

[0013] 2. Fermentation medium: glycerol 1-10%, potassium dihydrogen phosphate 1-8%, potassium chloride 1-2%, ammonium sulfate 0.3-3%, potassium hydroxide 0.5-5%, biotin 0.02-0.2% , the rest is water;

[0014] 3. The trace element is PTM1 type, and the added amount is 0....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for preparing phytase using glycerol as a carbon source, adopting the fermentation production process of phytase-producing genetically engineered bacteria using Pichia pastoris as a carrier, characterized in that glycerol is used as a carbon source, and an appropriate amount of bacterial strains for production is used , cultured in a shake flask in a seed medium, and then through the first-level seed culture, second-level seed culture, and fermentation culture processes, the only carbon source in the growth stage of the bacteria before the seed culture and fermentation enzyme production is glycerol; during fermentation, After the glycerol in the basic fermentation medium is completely consumed, start to feed 50% glycerol solution into the medium. After the glycerol is exhausted, start to feed methanol to induce phytase production. Methanol also acts as a carbon source in the production stage. The positive effect is: Glycerin as a carbon source increases the final phytase production by 20-30%, increases the enzyme activity by 20-30%, the color of the fermentation liquid is light and does not require decolorization treatment, and reduces production costs. This method reduces costs compared with traditional processes About 1 / 3.

Description

technical field [0001] The invention relates to a fermentation technology of microbial products and a method for preparing phytase using glycerol as a carbon source. Background technique [0002] The traditional method of producing phytase is to use glucose as carbon source. Glucose, as a carbon source, produces a caramelization reaction during the sterilization process, making the fermentation medium solution brown or soy sauce-colored. Its disadvantages are: first, the caramelization reaction produces harmful substances, which is not conducive to the growth of yeast; second, the post-processing of the dark fermented liquid requires decolorization, which increases the production process and costs. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing phytase using glycerol as a carbon source, which can significantly increase the output of phytase by 20%-30%, without decolorization, and reduces production costs. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12N1/32
Inventor 韩国宝王武良
Owner LIAONING HUAXING BIOTECHOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com