76results about How to "The result is intuitive and accurate" patented technology

The present disclosure belongs to the field of offshore oil, and in particular relates to a method for assessing the safety integrity level of offshore oil well control equipment. The method for assessing the safety integrity level of the offshore oil well control equipment comprises three major steps: creating a safety instrumented function evaluation module and dividing the related devices for performing the safety instrumented functions into a sensor subsystem; a controller subsystem and an actuator subsystem, establishing a dynamic Bayesian network model for respective subsystems for calculation; and integrating, analyzing and optimizing the safety integrity data of the subsystems.

The present invention discloses a multiaxial creep-fatigue prediction method based on ABAQUS, which comprises: S1: establishing an ABAQUS finite element model, and defining the viscoplastic constitutive equation of the material to be tested by means of the user subroutine UMAT; S2: determining the model parameters required by the viscoplastic constitutive equation; S3: establishing the fatigue damage calculation model and creep damage calculation model of the multiaxial stress-strain state of the material to be tested; S4: establishing an ABAQUS finite element model under the multiaxial stress-strain state, and calculating the stress-strain tensor of each cycle based on the defined viscoplastic constitutive equation and the model parameters; S5: calculating the equivalent stress and equivalent plastic strain by means of the user subroutine USDFLD, and superimposing the fatigue damage and creep damage of each cycle according to the linear cumulative damage criterion to obtain the crack initiation life of the material to be tested based on the fatigue damage calculation model and creep damage calculation model in combination with the stress-strain tensor.

The invention provides a detecting system and method for a partial discharge detecting sensor of GIS. The method comprises steps as follows: detecting and verifying the GIS partial discharge detecting sensor; detecting and verifying a GIS partial discharge UHF (ultra high frequency) sensor; and detecting and verifying a GIS partial discharge ultrasonic and UHF sensor positioning system. The system comprises a system GIS tank, a high-voltage sleeve, grading rings, basin-type insulators, a built-in camera, typical GIS defect models, a standard UHF sensor, a to-be-detected UHF sensor, an ultrasonic signal injection port, a UHF signal injection port, ultrasonic sensors, an ultrasonic partial discharge positioning system and a UHF partial discharge positioning system. The system and the method solve the problems that performance differences of ultrasonic sensors and UHF sensors of manufacturers cannot be compared and the performance of GIS partial discharge detecting systems of the manufacturers cannot be compared.

The invention discloses an optical rotation angle field detection device based on polarized beam splitting imaging and a measurement method thereof. The device comprises a polarized light generating device, a polarized beam splitting device, a light receiving and imaging device, an image acquisition device and a data processing system, wherein the polarized light generating device emits a bundle of linearly polarized light; the linearly polarized light is vertically incident to the polarized beam splitting device and forms two bundles of linearly polarized light after emergence from the polarized beam splitting device; the two bundles of light are respectively received by the light receiving and imaging device; and then, the light intensity information of the two bundles of light is transferred to the data processing system through the image acquisition device and is processed by the data processing system to obtain an optical rotation angle field. In the invention, the detection process is free from manual adjustment, the operation is simple, and errors caused by manual adjustment can be avoided; a two-dimensional field in a polarization state can be obtained directly by adoptingan imaging and image processing algorithm, the detection result is more visual and accurate and is free from the effect of light source fluctuation.

The invention relates to a method for identifying leech by one group of specific primers and belongs to the technical field of traditional Chinese medicine identification. The method comprises the following steps: firstly, preparing a DNA test sample solution; secondly, taking the DNA test sample solution as a template, selecting specific nucleotide sequence obtained through design and screening as primers to perform PCR amplification; thirdly, performing agarose gel electrophoresis analysis and gel imaging system detection on products; and finally, judging the true and false and the adulteration condition of the leech according to the detection result. The specific primer amplification method is simple in operation, sequencing is not needed, the result is intuitive, and the adulteration product can be detected and identified; meanwhile, the method is high in anti-interference ability and high in repeatability, can be used for accurately identifying leech foundation and medicinal materials, provides an efficient and rapid method for detecting the leech products, and has a relatively good application value.

The invention provides a method for detecting leveling property of battery slurry, which is used for measuring the flowing distance of the battery slurry with the set volume in a set inclined path and at set time, and then measuring the leveling property of the battery slurry according to the flowing distance. The method detects the leveling property of the battery slurry by the aid of the flowing property of the battery slurry. During detection, only the flowing distance of the quantitative slurry in the set inclined path and at the set time needs to be measured, if the flowing distance is longer, the leveling property is better, and the density of a polar plate surface prepared by the slurry is more uniform. The method is simple and convenient in operation and visual and accurate in results. Technicians in the field can determine upper and lower limit values of the flowing distance of a system of the standard slurry in advance, and when the slurry is blended, testing is performed according to the method until the flowing distance of the prepared slurry meets needs within a specified range.

A method and apparatus for identifying a concrete crack includes: obtaining a crack video, and manually annotating a video image frame by using a label; predicting a future frame and label for the annotated frame by using a spatial displacement convolutional block, propagating the future frame and label, to obtain a synthetic sample, and preprocessing the synthetic sample, to form a crack database; modifying input and output ports of data of a deep learning model for video semantic image segmentation and a parameter, to enable the deep learning model to accept video input, and establishing a concrete crack detection model based on the video output; using a convolutional layer in a trained deep learning model as an initial weight of the concrete crack detection model for migration; inputting the crack database into a migrated concrete crack detection model, and training the concrete crack detection model for crack data.

The invention discloses a kit with RT-LAMP nucleic acid test strips for detecting a porcine epidemic diarrhea virus and applications of the kit. The kit comprises a primer group of a nucleic acid represented by SEQ ID No. 1-6 and test strips for detection of nucleic acid. The usage of the kit is as follows: first preparing a RT-LAMP reaction system comprising an AMV retrovirus, a 1* reaction buffer, a strand displacement DNA polymerase, a dNTP mixture, betaine, MgSO4, a FIP primer, a BIP primer, a LoopF primer, a LoopB primer, a F3 primer, a B3 primer and RNA of a sample to be measured; carrying out a reaction at a constant temperature, after testing the obtained products by using the nucleic-acid-detecting test strip, judging and reading directly: the positive result is that two red strips appear, and one strip is in the detection zone while the other strip is in the control zone. The kit has advantages of simple operation, low cost, easy observation of the reaction result, good specificity, easy popularization and application in large scope and being extremely suitable for export quarantine, food hygiene and on-site detection in animal husbandry.

The invention discloses a detection method for tellurium zinc cadmium wafer damage layer thickness. Chemical gradient thinning is performed on the B surface of a water (111) for 10-30min by using a bromine methanol solution of volume fraction of 1%-5% firstly so that a sample of which the surface is an inclined surface is acquired; and then wafer defects are disclosed by using Everson corrosive liquid, observation is performed by using an optical microscope under the field of view of 100 times, the position at which a damage layer is just completely removed is confirmed, difference of wafer thickness before and after removal of the damage layer is compared and thickness of the damage layer is calculated. Advantages of the method are that (1) thickness of the damage layer is judged via observing morphology of the defects of the surface of the wafer so that the result is visual and accurate; (2) and wafer damage layer thickness detection can be completed within 1h without multiple times of corrosion or multiple times of detection so that the method is convenient, rapid and high in practicality.

The invention relates to the field of structural damage recognition, in particular to a structural damage early warning method based on empirical mode decomposition. The method comprises the steps that a vibration transmissibility function is constructed by utilizing structural response; an amplitude value of the vibration transmissibility function serves as an analysis signal; a natural mode function is obtained by an empirical mode decomposition technology; a waveform index of the natural mode function is calculated by reference to a definition of a waveform index in a signal waveform processing technology; waveform indexes before and after structural damage are compared to judge whether a structure is damaged; and structural damage early warning is performed. The method is high in operability; the processing precision is improved; and the early warning accuracy is improved.

The invention discloses a method for determining the non-linear HIFU (High Intensity Focused Ultrasound) sound field of biological tissues, which belongs to the technical field of sound field measurement. The method mainly comprises the following steps of: (a) transmitting a signal through a pulse receiving-transmitting instrument; (b) calibrating an energy transducer; (c) exciting a dual-frequency ultrasonic signal in a sample to be tested; (d) receiving the signal, sampling at equal intervals on different space and time domains, and performing FFT (Fast Fourier Transform) processing on the signal; (e) calculating the attenuation and dispersion of a sample material, smoothing a signal windowed function, and processing the signal by using two-dimensional Fourier transform to obtain the dispersion and attenuation spectrum of an ultrasonic signal; and (f) simulating the sound field in the sample by using a computer through a dispersion and an attenuation value obtained in the step (e). Due to the adoption of the method, the sound wave condition of sound filed transmitted in the biological tissues can be determined really and accurately; and the method has the characteristics of simple measuring method and high result precision.

The present invention provides a method for judging emotions based on heart rate and respiration. The method at least includes: monitoring the heart rate and respiration of several experimenters for a period of time; Action; record the different emotional states of the experimenter in the time period and the heart rate and breathing rate under the emotional state; make the different emotional states and the heart rate and breathing rate corresponding to the emotional state into a data table to obtain an emotional evaluation report ; Determining the emotion; adopting the monitoring of the heart rate and breathing rate to the judged emotional person; substituting the obtained data into the emotional evaluation report to obtain the emotional state of the judged person. This method overcomes the problem of measuring the fluctuation of emotions in the past, but it is difficult to distinguish whether the fluctuation is positive emotion or negative emotion. The process is simple, the monitoring is accurate, and the result is intuitive.

The invention relates to a test paper for quickly determining peracetic acid disinfection liquid and a preparation method, and belongs to the field of sanitary disinfection medicines. The test paper is characterized in that the test paper is treated by a solution containing potassium iodide and a stabilizer, and the mass ratio of sodium thiosulfate to sodium carbonate to polyvinyl alcohol in the solution is 1:(0.5 to 5):(0.25 to 2.5). The preparation method comprises the following steps of completely soaking the test paper into mother liquid, soaking for 3 to 5min, fetching out, drying at the temperature of 50 to 70 DEG C, and cutting into proper sizes after the test paper is completely dried, so as to obtain the test paper. The operation method has the advantages that the operation is simple and convenient, the result is clear and visual, the clinical requirement on the concentration and accuracy of the peracetic acid disinfection liquid is completely met, any special technical requirement on an operator is avoided, and the disinfection result of the used peracetic acid disinfection liquid can be visually and quickly evaluated.

The invention relates to the technical field of genetic relationship identification methods, and discloses a genetic relationship identification method with SNP as a genetic marker. The method comprises the following three steps of: establishing a genetic relationship judgment model, carrying out typing detection on SNP sites, and confirming the genetic relationship among individuals according toa typing result and the judgment model. According to the genetic relationship identification method with the SNP as the genetic marker, the SNP is used as the genetic marker in replacement of STR in the detection mode, so the defects of poor stability, high typing difficulty, high sample quality requirements and high cost when the STR is used as the genetic marker for detection are fully avoided;whether two samples are of a direct line, or are in a first-level genetic relationship, a second-level genetic relationship or a third-level genetic relationship, or are strangers can be directly judged through a new model algorithm; a probability density model is introduced, so the influence caused by various errors is fully avoided; programmed judgment is adopted, so operation is easy and convenient; an SNP typing detection technology is adopted, so typing results are accurate and visual; and a great number of SNP sites are used by the algorithm, the number of repetitions is high, and the result is more accurate.

The invention discloses an image matching-based foreground segmentation method and device, and relates to an image matching technology and an image segmentation technology in the field of multimedia. The image matching-based foreground segmentation method is characterized by comprising the following steps of: respectively extracting local features of two input images and matching key points according to the extracted local feature information; screening wrong matching points from matching points of the obtained key points so as to obtain all the correct matching points; obtaining a feature point group on a foreground target from all the correct matching points by using clustering analysis; and obtaining the foreground target in a picture by utilizing a picture segmentation algorithm according to the obtained feature point ground. The invention furthermore discloses the image matching-based foreground segmentation device. By adoption of the image matching-based foreground segmentation method and device, foreground targets in pictures can be objectively obtained, so that the results are more accurate and intuitional, the traditional man-machine interaction method can be replaced, the whole time is decreased and the efficiency is improved.

The invention provides a kit for quickly detecting water-soluble explosives and a detecting method of the kit. The kit comprises 13 detection reagents; various cations, anions and molecular compounds in the water-soluble explosives and explosive residuals can be detected quickly and accurately by the kit; and the detection varieties include nitrate, nitrite, chlorate, ammonium, urea, permanganate, chloride ions, sulfur ions, sulfate, phosphate, sugar, sodium ions and potassium ions. The kit provided by the invention can be used for on-site explosive searching and quick determination of the nature of an explosive case, has the characteristics of simplicity, quickness, strong specificity, sensitive response, convenience in use and low cost, and can provide an effective technological means for the criminal investigation department of the public security system to solve explosive cases. The kit not only can be used for quickly detecting traditional explosives and explosive residuals, but also can be used for quickly and effectively detecting special explosives and explosive residuals in Xinjiang region.

The invention provides an evaluation method of in-vitro natural killer cell immunocompetence and application thereof. The evaluation method comprises the following steps of: co-culturing a target cellcarrying a fluorescence label A and a natural killer cell, dyeing and labelling dead cells generated after co-culture by using a fluorescence label B, and carrying out microscopic imaging on the dyedand labelled cells by using a bright field, a fluorescence channel matched with the fluorescence label A and a fluorescence channel matched with the fluorescence label B respectively so as to obtaina microscopic image, recognizing cells in the obtained microscopic image through image recognition, performing superposition synthesis analysis on recognition results of the same region, and evaluating the immunocompetence of the natural killer cells according to an analysis result. According to the invention, a direct reading method for directly obtaining a detection result through an image is adopted, so that the detection result is more accurate and visual; and thus, the method is suitable for the fields of medical diagnosis and biomedical industrialization with higher standardization requirements.

The invention relates to the field of medical instruments, and particularly discloses a high-definition colonoscopy system. The high definition colonoscopy system comprises a main controller provided with a displayer. The high-definition colonoscopy system is characterized in that the main controller is connected with an endoscope through a data line, the endoscope comprises a handle with the end provided with a microscope, the front end of the lens of the microscope is connected with a connecting pipe through a bayonet, the bayonet is internally provided with an optical fiber circuit connected with the main controller, and the connecting pipe is sleeved with a disposable telescope. The high-definition colonoscopy system is simple in structure, convenient to use, reasonable in design and flexible in application, and has the functions of freezing, playing back, collecting, storing, measuring focuses, the lesion states of the interior the enteric cavity can be clearly detected, the result is accurate and visual, and the high-definition colonoscopy system is suitable for being widely applied to anus and anal tube disease detection.

The invention discloses a real-time fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for detection of foot and mouth disease virus and seneca valley virus and application. The kit comprises primers and probes for detecting the foot and mouth disease virus and the seneca valley virus and preferably further comprises nucleic acid extract liquid, 2*Direct qRT-PCRMix, enzyme mixed liquid, negative control and positive control. By adoption of the kit for detecting the foot and mouth disease virus and the seneca valley virus, high specificity, high sensitivity,high stability, simplicity and convenience in operation and the like are achieved. Without extra extraction of virus RNA and reverse transcription, a user only needs to add a to-be-tested sample intoa reaction tube, then performs quantitative analysis on a start template according to fluorescence signal changes and a Ct value and standard curve relation and finally calculates a copy number of the to-be-tested sample. The kit is not only applicable to quantitative analysis in research and development institutions but also suitable for pathogen detection and analysis in all levels of prevention and control institutions, basic veterinary stations, large and medium sized farms and the like, thereby having a promising application prospect.

The invention discloses a PCR primer set, site, method and kit for minimally invasive and rapid identification of the ZW sex chromosome type of pelodiscus sinensis. The sequence of the primer set is shown as SEQ ID No.1 and SEQ ID No.2. With a trace amount (0.5-1 microliter) of pelodiscus sinensis blood as a starter template, the primer set is used for carrying out PCR amplification, an amplification product is subjected to electrophoresis detection, and when 2031 bp bands appear in the amplification product, it is determined that the pelodiscus sinensis is in a ZZ sex chromosome type; when 2031 bp and 1624 bp bands appear in the amplification product, it is determined that the pelodiscus sinensis is in a ZW sex chromosome type. With the trace amount of blood obtained by minimally invasivecollection as a starter material, the sex chromosome type of the pelodiscus sinensis is rapidly detected; the detection hardly damages tested individuals, it can be ensured that pelodiscus sinensis in each growth and development stage can survive healthily after sampling, and an important tool is provided for identifying the sex hromosome type in the pelodiscus sinensis monosex culture technologyand sex-controlled breeding.

The invention relates to test paper for measuring the content of available chlorine, belonging to the technical field of chemical indicators. The test paper is used for detecting the concentration of chemical disinfectants and is beneficial to monitoring the concentration of the disinfectants in the use process in the hospitals and related industries. The test paper is prepared by soaking chromatography filter paper into an indicator and then drying the filter paper, wherein the indicator comprises the following ingredients by weight: 1-3% of o-tolidine hydrochloride, 35-50% of citric acid, 1-3% of sodium dodecyl sulfate, 1-2% of hydroxyethylcellulose and the balance of water; and the indicator is prepared by adding the citric acid to water to prepare citric acid aqueous solution, then adding the o-tolidine hydrochloride, the sodium dodecyl sulfate and the hydroxyethylcellulose to the citric acid aqueous solution and stirring the mixture to totally dissolve the three ingredients. The test paper has the advantages of simpleness and convenience in use and intuitive and accurate results.

The invention discloses a paper chip for rapidly detecting organophosphorus pesticide. The paper chip comprises test paper, an electrode, an immobilized reaction enzyme and a gold finger interface connected with the electrode, wherein a hydrophobic layer is selectively coated on the surface of the test paper to reserve a reaction area uncoated by the hydrophobic layer and a sample feeding channel on the surface of the test paper; a cofferdam formed by the hydrophobic layer is arranged around the reaction area; the sample feeding channel is led to the reaction area from the edge of the test paper; the detection end of the electrode is arranged in the reaction area; the other end of the electrode is connected with the gold finger interface formed in the end of the test paper; the immobilized reaction enzyme is coated on a junction of the reaction area and the sample feeding channel. When the paper chip is matched with a special detection instrument, the paper chip is capable of reflecting whether residues in a detected solution exceed the standard through direct degrees of the detection instrument according to the potential change generated by a reaction of the reaction enzyme and the detected solution; the paper chip is simple, convenient and rapid to operate, intuitive and accurate in the results and low in the cost; the test paper can be used without the influence of light and environment; the test paper can be taken together with a handheld detection device to carry out rapid detection outdoors.

The invention discloses a high-precision detection method for seismic signal coherence, wherein the high-precision detection method is improved on the basis of a Gaussian kernel function and a higherderivative. On the basis of an eigenvalue coherence algorithm, also called as a third generation coherence algorithm, a third generation coherent data volume is convoluted with the Gaussian kernel function, a second-order partial derivative of the obtained new data volume on a main line and a crossline and in time direction is solved, maximum eigenvalue of data after derivation is solved, and themaximum eigenvalue is taken as a final result of an improved algorithm, so that a new coherent result is obtained. The high-precision detection method disclosed by the invention is used for detectingsimilarity, namely coherence, of a seismic signal, so that the high-precision detection method is applied to identification and prediction of a subsurface fault, a fracture-developed zone and some sedimentary characteristics; meanwhile, the high-precision detection method disclosed by the invention can more clearly show spatial distribution of a fault and reflect detail characteristics of a subsurface structure and a sedimentary environment, so that a new promoting effect is produced to scientific research.

The present invention relates to primers and a method for detecting related gene SNP (single nucleotide polymorphism) sites of metformin-individualized medications. The primers can detect a rs2289669SNP site of a SLC47A1 gene. The primer sequences comprise a wild-type upstream primer, a mutant-type upstream primer and a shared downstream primer. Improvement is made on the basis of ARMS-PCR, a specific primer and the shared downstream primer are designed for SNP sequences, mismatched bases are introduced at a second position of a 3' end of the specific primerto widen a gap between amplification efficiency of complementation and non-complementation of the bases of the 3' end of the specific primer and SNP site bases on a DNA template, thereby greatly improving specificity and accuracy of detection. A real-time PCR technology is introduced, identification can be made according to a [delta]Ct value, that is, the [delta]Ct value is equal to mutant Ct-wild Ct. In general, when [delta]Ct islarger than 7, the SNP site is a wild type, when [delta]Ct is less than -7, the SNP site is a mutant type, and when the [delta]Ct is larger than -2 and less than 2, the SNP site is a heterozygous type. Advantages are that the method is fast, simple, economical, accurate and reliable identification of results, and high in specificity.

The invention provides a recombinant protein. The amino acid sequence of the recombinant protein is represented by SEQ ID NO.1. The invention further provides a gene represented by SEQ ID NO.2. The invention further provides application of the protein in detection of a porcine epidemic diarrhea virus antibody. The protein is mainly used as a coating antigen. The invention further provides a kit for detecting porcine epidemic diarrhea virus antibody. The kit contains the recombinant protein, coating liquid, washing liquid, closing liquid, diluent, goat anti-pig IgG marked by horse radish peroxidase and a primer developing solution. The recombinant protein represented by SEQ ID NO.1 is used as the coating antigen for detecting the porcine epidemic diarrhea virus antibody by virtue of an indirect ELISA method and has relatively good specificity, sensitivity and repeatability, and a result is intuitional and accurate.

The invention discloses primers and a kit used in loop-mediated isothermal amplification detection method of PML-RAR alpha fusion gene and the primers and the kit are used for solving the problems in conventional detection of leukemia minimal residual disease that false positive easily appears, the detection cost is high, the operation is complex and the detection time is long. According to the sequence of the PML-RAR alpha gene, multiple groups of primers are designed by virtue of PrimerExplorer V4 software, a pair of specific inner primers F3 and B3 and a pair of specific outer primers FIP and BIP are screened and the kit for detection of PML-RAR alpha gene in the acute promyelocytic leukemia minimal residual disease is established. The kit has the advantages of high amplification efficiency, good specificity and low requirement on instruments and equipment, a constant-temperature water bath pot is only needed, which is easily realized in clinic, the detection time is not more than one hour, which is less than a half of the detection time of the conventional PCR.

The invention discloses an immunofluorescence detection method for rapidly measuring the virus carried rate of vector insects on a large scale. The method comprises the steps of classifying the vector insects collected in the field, dissecting, fixing, permeating, preparing antibody labeling liquid, incubating antibody, carrying out slide preparation, observing, and calculating the number of insects carrying virus by statistics to obtain the virus carried rate of the vector insects. The method is low in detection cost, only consumes four hours, is visual and accurate in detection result and high in sensitivity, and can be used for detecting the virus carried rate of the vector insects in the field on a large scale.

The invention relates to the technical field of analysis and detection, and provides a method for identifying the quality difference of thin-skinned melon fruits after application of forchlorfenuron.The method comprises the following steps of detecting and analyzing volatile organic compounds in a thin-skinned melon fruit sample after application of forchlorfenuron based on a headspace-gas chromatography-ion mobility spectrometry technology, and determining a characteristic peak comparison diagram corresponding to the volatile organic compounds through gas chromatography retention time and ion mobility time; and screening symbolic differences from the measured volatile organic compounds by adopting a partial least squares discriminant analysis method, and carrying out semi-quantitative comparative analysis, thereby carrying out rapid and accurate difference analysis on the quality of the thin-skinned melon fruits after application of forchlorfenuron. The method is advantaged in that gas chromatography (GC) and ion mobility spectrometry (IMS) are combined, the outstanding separation characteristic of GC and advantages of quick response and high sensitivity of IMS are utilized, andthe identification method further has properties of low detection cost, high sensitivity, simple and convenient process, simple sample pretreatment, high analysis speed and visual result.

The invention relates to a triazophos monoclonal antibody. The triazophos monoclonal antibody is characterized in that the amino acid sequence of a heavy-chain variable region of the antibody is shownas SEQID NO:1, and the amino acid sequence of a light-chain variable region of the antibody is shown as SEQID NO:2. The invention relates to an application of the triazophos monoclonal antibody to colloidal gold detection test paper. The colloidal gold detection test paper comprises a liner, as well as a sample pad, a gold marker combination pad, a cellulose membrane and a water absorption pad which are sequentially arrayed on the liner, wherein the inner part of the gold marker combination pad is adsorbed with a triazophos gold marker antibody, a detection line and a control line are formedin the cellulose membrane, the detection line is printed with a triazophos hapten coupled carrier protein solution, carrier protein adopted in the carrier protein solution is prepared through couplingO-ethyl-O-(1-phenyl-1,2,4-triazole-3-based)-N-(butyric acid based) thiophosphate ester with bovine serum albumin (BSA) in an active ester method, and the control line is printed through a rabbit antimouse IgG antibody. The triazophos monoclonal antibody disclosed by the invention is high in specificity, high in sensibility, simple, convenient and quick to detect, vivid, audio-visual and accuratein result display, wide in application range and convenient to popularize, and fees are saved.

The invention relates to a chlopyrifos monoclonal antibody and a preparation method and application thereof. The chlopyrifos monoclonal antibody and preparation method and application thereof have theadvantages that the specificity and sensitivity are high, the method is simple and rapid, and a result is displayed vividly, visually and accurately; the cost is reduced, the application range is wide, convenience is provided for popularization; and via the prepared chlopyrifos colloidal gold test paper, the result can be determined within minutes according to strips presented after reaction needless of professionals or auxiliary instruments, operation is simple and rapid, the result is visual and accurate, the cost is low, and detection can be carried out in the outside.