Paper chip for rapidly detecting organophosphorus pesticide and production method thereof

Abstract

The invention discloses a paper chip for rapidly detecting organophosphorus pesticide. The paper chip comprises test paper, an electrode, an immobilized reaction enzyme and a gold finger interface connected with the electrode, wherein a hydrophobic layer is selectively coated on the surface of the test paper to reserve a reaction area uncoated by the hydrophobic layer and a sample feeding channel on the surface of the test paper; a cofferdam formed by the hydrophobic layer is arranged around the reaction area; the sample feeding channel is led to the reaction area from the edge of the test paper; the detection end of the electrode is arranged in the reaction area; the other end of the electrode is connected with the gold finger interface formed in the end of the test paper; the immobilized reaction enzyme is coated on a junction of the reaction area and the sample feeding channel. When the paper chip is matched with a special detection instrument, the paper chip is capable of reflecting whether residues in a detected solution exceed the standard through direct degrees of the detection instrument according to the potential change generated by a reaction of the reaction enzyme and the detected solution; the paper chip is simple, convenient and rapid to operate, intuitive and accurate in the results and low in the cost; the test paper can be used without the influence of light and environment; the test paper can be taken together with a handheld detection device to carry out rapid detection outdoors.

Description

technical field [0001] The invention relates to a quick detection device and a manufacturing method thereof, in particular to a paper chip for rapid detection of organophosphorus pesticides and a manufacturing method thereof, belonging to the technical field of rapid detection equipment. Background technique [0002] At present, in the detection of pesticide residues, instrument detection such as gas chromatography and high performance liquid chromatography are still the most widely used detection techniques. However, traditional detection methods of this type usually require expensive instrumentation and can only be performed in a laboratory setting. At present, according to the national standard for the rapid detection of organophosphorus pesticide residues in vegetables, the widely used rapid test card for pesticide residues is a quick test card made of cholinesterase and chromogenic reagents that are highly sensitive to organophosphorus pesticides. It is not easy to sav...

AI Technical Summary

Problems solved by technology

However, traditional detection methods of this type usually require expensive instrumentation and can only be performed in a laboratory setting

At present, according to the national standard for the rapid detection of organophosphorus pesticide residues in vegetables, the widely used rapid test card for pesticide residues is a quick test card made of cholinesterase and chromogenic reagents that are highly sensitive to organophosphorus pesticides. It is not easy to store, and the operation needs to be squeezed manually, which is difficult, increases randomness and uncertainty, and is easily affected by temperature, and the detection takes a long time

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