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Method for identifying quality difference of thin-skinned melon fruits after application of forchlorfenuron

A thin-skinned melon, quality difference technology, applied in the field of analysis and detection, can solve the problems of inability to provide scientific basis for the impact of forchlorfenuron on the quality of melons, low efficiency, and inability to accurately identify fresh thin-skinned melons

Active Publication Date: 2020-10-27
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, agricultural product quality analysis methods, such as traditional sensory analysis, mainly rely on the human body itself, which has a certain degree of subjectivity and will produce different results due to factors such as changes in the evaluation environment and the evaluator's own conditions. Low disadvantages; and the sensory analysis method cannot accurately know the difference in volatile organic compounds of thin-skinned melons after the application of forchlorfenuron, the judgment results are not accurate, and cannot provide a scientific basis for the impact of forchlorfenuron on the quality of melons
Other quality control methods (such as gas chromatography-sniffing-mass spectrometry) also have the disadvantages of complicated process, many pre-treatment procedures, and long time-consuming, and cannot accurately identify the quality of fresh thin-skinned melons.

Method used

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  • Method for identifying quality difference of thin-skinned melon fruits after application of forchlorfenuron
  • Method for identifying quality difference of thin-skinned melon fruits after application of forchlorfenuron
  • Method for identifying quality difference of thin-skinned melon fruits after application of forchlorfenuron

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Embodiment 1

[0055] Step S101: Homogenize the thin-skinned melon after removing the stems and seeds, and obtain a fresh homogeneous thin-skinned melon sample. Weigh 2 g of the fresh homogeneous thin-skinned melon sample, put it in a 20 mL headspace bottle, and incubate at 40°C for 20 minutes Headspace sampling; according to the order of CK, 10mg / kg, 15mg / kg, and 20mg / kg, sequentially inject samples (after the analysis of the previous sample is completed, inject the next sample), repeat five times (that is, inject each sample Sample 5 times), to reduce the difference between the treatment groups caused by different injection time.

[0056] Step S102: using headspace-gas chromatography-ion mobility spectrometry to analyze the thin-skinned melon sample to obtain a gas-phase ion mobility spectrum of volatile organic compounds in the thin-skinned melon;

[0057] Gas chromatography conditions: chromatographic column type FS-SE-54-CB-1 quartz capillary column (15m×0.53mm, 1μm); analysis time 30mi...

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Abstract

The invention relates to the technical field of analysis and detection, and provides a method for identifying the quality difference of thin-skinned melon fruits after application of forchlorfenuron.The method comprises the following steps of detecting and analyzing volatile organic compounds in a thin-skinned melon fruit sample after application of forchlorfenuron based on a headspace-gas chromatography-ion mobility spectrometry technology, and determining a characteristic peak comparison diagram corresponding to the volatile organic compounds through gas chromatography retention time and ion mobility time; and screening symbolic differences from the measured volatile organic compounds by adopting a partial least squares discriminant analysis method, and carrying out semi-quantitative comparative analysis, thereby carrying out rapid and accurate difference analysis on the quality of the thin-skinned melon fruits after application of forchlorfenuron. The method is advantaged in that gas chromatography (GC) and ion mobility spectrometry (IMS) are combined, the outstanding separation characteristic of GC and advantages of quick response and high sensitivity of IMS are utilized, andthe identification method further has properties of low detection cost, high sensitivity, simple and convenient process, simple sample pretreatment, high analysis speed and visual result.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for identifying the difference in fruit quality of thin-skinned muskmelon after forchlorfenuron is applied. Background technique [0002] Thin-skinned melon (Cucumis melon L.) is widely cultivated in various parts of China because of its unique flavor and rich nutrients. In recent years, forchlorfenuron, as a synthetic plant growth regulator, has been widely used for fruit setting or yield increase of thin-skinned melons. The report shows that after the application of forchlorfenuron, the quality of thin-skinned muskmelon will be different, which will further affect its market attributes, and then have a greater impact on the thin-skinned muskmelon industry. With the increasing use of forchlorfenuron in agricultural production, the possible impact of its application on crop quality has become a hot spot of social concern and research. To identify the thin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 金芬王琦苏杭
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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