The invention discloses an anti-infection wound-repairing pharmaceutical composition in the technical field of biomedicines. The anti-infection wound-repairing pharmaceutical composition is prepared from the following raw materials in parts by weight, 30-40 parts of chitosan hydrochloride, 80-120 parts of cunninghamia lanceolata, 30-36 parts of bletilla striata, 2-8 parts of borneol and 450-550 parts of glycerol. The pharmaceutical composition is applied to prepare a film spraying preparation, and the preparation method comprises the following steps, step 1, weighing the raw materials in proportion, processing cunninghamia lanceolata and bletilla striata, and grinding the processed cunninghamia lanceolata and bletilla striata into fine powder; step 2, adding PVA1788 and chitosan hydrochloride to distilled water under water bath condition at 40 DEG C, and conducting magnetic stirring to be completely dissolved; step 3, adding cunninghamia lanceolata powder, bletilla striata powder and borneol, then adding glycerol, continuously conducting magnetic stirring to be completely dissolved, and standing at room temperature until foams are eliminated; step 4, adding absolute ethyl alcohol,conducting uniform stirring, standing for 5 minutes, and conducting bottling; and step 5, conducting sub-packaging and ultraviolet sterilization. The pharmaceutical composition disclosed by the invention has the effects of effusion resistance, adhesion prevention, hemostasis, anti-infection, promotion of wound healing, etc.