31results about How to "Determine content" patented technology

The invention discloses a preparation and use method of a ratio fluorescent probe for visual distinguishing detection. The preparation method specifically comprises the following steps: step 1, preparation of silicon-based carbon quantum dots; step 2, preparation of the nano mesoporous molecularly imprinted material coated with the silicon-based carbon quantum dots; step 3, performing surface modification on the nano mesoporous molecularly imprinted material; and step 4, preparing the ratio fluorescent probe based on the nano mesoporous molecularly imprinted material. The use method comprises the following steps: (1) diluting tetracycline or oxytetracycline into solutions with different gradient concentrations; (2) dispersing the prepared ratiometric fluorescent probe based on the nano mesoporous molecularly imprinted material in ultrapure water; (3) adding a Tris-HCl buffer solution with the pH value of 8 into a quartz cuvette, uniformly mixing the Tris-HCl buffer solution and the solution, and respectively dropwise adding the tetracycline solution or the oxytetracycline solution in the step (1) for fluorescence determination; the main substrate is the silicon dioxide molecular imprinting layer with the mesoporous structure, the method is reliable, mass production can be carried out, and the probe is widely applied to the society.

It is intended to provide a mixture for preventing the diffusion of a contaminating component which has a stable and excellent purification ability against a contaminating component contained in contaminated soil compared with the existing techniques for preventing the diffusion of a contaminating component; and a method of preventing the diffusion of a contaminating component. A mixture for preventing the diffusion of a contaminating component comprising an adsorbent which adsorbs the contaminating component contained in contaminated soil and a diluting agent which is blended with the above-described adsorbent at a definite ratio to control the coefficient of water permeability and the thickness, characterized in that the mixture shows a space velocity of water containing the contaminating component as described above of from 1 to 60 (l / hr).

The invention discloses a method for multistep quantitatively detecting serum albumin and metaglobulin in plasma, belonging to the field of bioluminescence marking. The method comprises the following steps: adding fluorescent reagents in different serum albumin concentrations into the fluorescent reagent at several times and measuring DP-TPPNa fluorescence intensity after dropwise adding each time; acquiring the optimal concentration and the detection scope of DP-TPPNa for serum albumin according to a measuring result; dropwise adding the serum albumin and metaglobulin in different mass ratios into the fluorescent reagent at several times and measuring DP-TPPNa fluorescence intensity after dropwise adding each time; drawing a change curve of change rate of DP-TPPNa fluorescence intensity following the concentration of serum albumin according to the measuring result; selecting a standard curve according to the change curve; adding a plasma sample into the fluorescent reagent, measuring the DP-TPPNa fluorescence intensity, substituting into a linear equation of the standard curve and calculating the concentration of the serum albumin and metaglobulin.

The present invention relates to a quantitative analysis method for a monomer of a color filter (CF) photoresist (PR) binder for a thin film transistor-liquid crystal display (TFT-LCD), and can perform quantitative analysis on a monomer of a CF PR binder for a RFT-LCD by using a Py-GC / MS used for qualitative analysis.

The invention discloses a standard colorimetric method for carbonyl compound content in industrial methanol, and belongs to the field of industrial methanol. The method comprises the following steps:preparing a series of standard solutions with different carbonyl compound contents, reacting the standard solutions with 2, 4dinitrophenylhydrazine and potassium hydroxide in sequence under certain conditions, and observing to find that the color is gradually changed from faint yellow to dark red brown along with the gradual increase of the carbonyl compound contents; preparing a standard colorimetric card according to the color of the standard solution, the volume part mass number of the carbonyl compound content, the methanol grade and other information; after the sample is completely reacted under the same condition, comparing the sample with a standard colorimetric card, finding out the closest color, and directly reading out information such as the content of the carbonyl compound andthe grade of a methanol sample. Quantitative calculation of the content of the carbonyl compound can be achieved. The determination method has the characteristics of intuition, rapidness, simplicity,convenience, low requirements on detection equipment, instruments, places and the like.

The present application discloses a detection reagent combination, a kit and a detection method for rubidium or cesium ions. The detection reagent combination includes component (a): a solution of gold nanoparticles modified by a modifier, or used to form the modified gold nanoparticle solution. A reagent component of an agent-modified gold nanoparticle solution, wherein the modifier is a thiol compound containing a benzene ring; Component (b): a ligand solution, or a reagent component for forming the ligand solution; the ligand The body solution is selected from at least one of dibenzo-21-crown-7 solution, dialkyldibenzo-21-crown-7 solution, and benzo-21-crown-7 solution. The detection method for rubidium or cesium ions provided in this application only uses naked eye colorimetry, that is, both can reach a minimum of 1×10 ‑5 The lower detection limit of mol / L; the method of the invention combined with the instrumental analysis means can also detect lower concentrations of rubidium or cesium ions, and has very high sensitivity.

The invention discloses the synthesis of an ionic liquid-based fluorescent hydrogel sensor and its application in detecting p-nitroaniline. The ionic liquid monomer and initiator azobisisobutyronitrile were dissolved in dimethyl sulfoxide to prepare a mixed solution, the mixed solution was adsorbed on the substrate material, cross-linked in situ under heating conditions, washed and dried to synthesize a fluorescent hydrogel sensor . The material can be used as a fluorescence sensor for rapid detection of p-nitroaniline, has the advantages of high sensitivity, short detection time, good selectivity, etc., and can be applied to the detection of p-nitroaniline in environmental living water samples.

The invention discloses a method for preparing and using a ratiometric fluorescent probe for visual discrimination detection. The preparation method specifically includes the following steps: Step 1, preparation of silicon-based carbon quantum dots; Step 2, nano-mesoporous coating of silicon-based carbon quantum dots Preparation of molecularly imprinted materials; Step3, surface modification of nano-mesoporous molecularly imprinted materials; Step4, preparation of ratiometric fluorescent probes based on nano-mesoporous molecularly imprinted materials; the method of use includes: (1) Dilute tetracycline or oxytetracycline Solutions with different gradient concentrations; (2) The prepared ratio fluorescent probe based on nano-mesoporous molecularly imprinted materials was dispersed in ultrapure water, (3) Tris-HCl with pH=8 was added to the quartz cuvette After the buffer solution and the solution are mixed, the tetracycline solution or the oxytetracycline solution in the step (1) are respectively added dropwise for fluorescence measurement; the main matrix of the present invention is a silicon dioxide molecularly imprinted layer with a mesoporous structure, and the method is reliable and can mass-produced and widely used in society.

The invention relates to a method for operating a water-conducting household appliance having a drum (3), a control device (18) and a spectrometer (21), the drum receiving objects (22, 34) to be treated, The drum has at least one circumference with at least one radiation channel (30, 35), the spectrometer comprises at least one radiation source (5, 27), at least one radiation detector (7, 28) and a microcomputer (6), wherein , the microcomputer (6) and the control device (18) are configured to exchange information with each other, so that data can be exchanged between the spectrometer (21) and the control device (18), it is characterized in that the method comprises the following steps: (a) The drum (3) is rotated at a predetermined rotational speed Dz, wherein the frequency f based on the number of revolution cycles of the radiation channels (30, 35) per unit time is defined as the ratio of the rotational speed Dz to the radiation channels (30, 35) The product of the quantities; (b) continuously emitted by at least one radiation source (5, 27) of the spectrometer (21) in the wavelength range λ 1 ‑λ 2 the radiation (31) in passes through at least one radiation channel (30, 35) of the rotating drum (3), so that a periodically interrupted radiation (31) with frequency f is produced in the drum (3), and the objects (22, 34) in the rotating drum (3) are loaded with radiation (31) periodically interrupted at a frequency f, and at least one radiation detector (7, 28) is arranged such that the objects (22, 28) 34) The reflected radiation (32) is recorded directly as a measurement signal without passing through the radiation channel (30, 35) and thus not being interrupted by the radiation channel (30, 35) at frequency f, and is generated by the control device (18) only in relation to A periodic function with a period length 1 / f - in particular a sinusoidal or rectangular function - associated with the household appliance (1) as a reference signal; (c) the measurement signal and the reference signal are transmitted to the microcomputer (6), with the aid of the stored in The analysis routine in the microcomputer (6) analyzes the measurement signal and compares it with the reference signal, and transmits the analyzed measurement signal to the control device (18); (d) via the control device (18) An operating program is selected and / or adapted with respect to the evaluated measurement signals, wherein steps (b) to (d) are carried out one or more times. Furthermore, the invention relates to a domestic appliance which is suitable for carrying out the method.

The embodiment of the invention discloses a method for determining active ingredients with myocardial protection and anti-inflammatory action in traditional Chinese medicine. The method comprises the steps of (1) obtaining each ingredient of the target traditional Chinese medicine through preparation of liquid chromatogram; (2) determining the active ingredient with myocardial protection action through myocardial cell antioxidant model experiment; (3) determining the active ingredient with anti-inflammatory action through macrophage anti-inflammatory model experiment. According to the determined traditional Chinese medicine active ingredients with myocardial protection and anti-inflammatory action, content of the traditional Chinese medicine active ingredients is easy to determine, then, quality control on the target traditional Chinese medicine can be realized, and clinical curative effect of the target traditional Chinese medicine is guaranteed.

The invention relates to a method for detecting EDTA (ethylene diamine tetraacetic acid) in a laundry detergent. The method comprises steps as follows: (1) preparing a potassium bicarbonate aqueous solution; (2) adding the to-be-detected laundry detergent to the potassium bicarbonate aqueous solution, stirring the solution at the temperature of 45-50 DEG C for 10-20 min, filtering the solution while the solution is hot, and naturally cooling the solution to the room temperature to obtain a pretreated solution; (3) adding an iron liquid to the pretreated solution, performing oscillating extraction and then centrifugation, and removing an aqueous phase to obtain an ion liquid phase; (4) adding trivalent iron salt to the ion liquid phase, after full and even mixing, performing chromatography separation with a gel column chromatography Superose 12 column, performing washing with n-hexane firstly, then performing gradient elution with a petroleum ether-normal propyl alcohol mixed solvent serving as an eluent, and collecting the eluent; (5) evaporation the solvent of the eluent until the solvent is almost dry, and performing dissolution, volume determination and filtration with an organic membrane to obtain a detection sample; (6) performing liquid chromatography separation on the to-be-detected sample to determine the content. The method is excellent in EDTA quantitation accuracy, and a new detection means is provided for control on the content of the EDTA in the laundry detergent.