30 results about "Mycoplasma antibody" patented technology

The invention relates to a biological detection reagent and a preparation method thereof, and particularly relates to a detection reagent for a mycoplasma bovis antibody and a preparation method thereof. The detection reagent for the mycoplasma bovis antibody is composed of one part of a mycoplasma bovis P48 recombined and expressed fusion protein antigen and one part of 10% hydroformylation-tanned sheep red blood cells (v / v) which are uniformly mixed. The gene sequence of the P48 recombined and expressed fusion protein antigen for the detection reagent for the mycoplasma bovis antibody is SEQ ID No.1. The detection reagent can be used for really detecting mycoplasma bovis and has the advantages of long preservation time, high sensitization titer, easiness in long-time preservation, high blood coagulation titer, simplicity in operation, convenience and rapidness, low cost, safety and stability, no toxin, no environmental pollution and the like.

The invention discloses a mycoplasma bovis antibody detection test strip and a preparation method thereof. The test strip provided by the invention comprises a bottom plate and a sample absorption pad, a colloidal gold pad coated with an antigen, a nitrocellulose membrane and a water absorption pad which are all arrayed on the bottom plate in sequence, wherein the nitrocellulose membrane is coated with a quality control line and a detection line; the detection line is coated with a Goat Anti-Bovine Secondary antibody IgG; the quality control line is a rabbit derived P48 fusion protein. The test strip provided by the invention can detect mycoplasma bovis antibody, including blood serum antibody and milk serum antibody, and has the advantages that the test strip is low in cost, simple to operate, convenient and quick to detect, requires no special equipment and apparatus, is high in detection susceptibility, strong in specificity, wide in application range and the like.

The invention relates to the technical field of biology, in particular to a method for detecting a mycoplasma pneumoniae antibody, a kit for detection by adopting the method and a preparation method of the kit. The method comprises the steps as follows: fixing anti-human IgM (immunoglobulin m) and anti-human IgG (immunoglobulin g) to a membrane respectively; adding a serum to be detected; after the mycoplasma pneumoniae antibody in the serum to be detected is bonded with the anti-human IgM and the anti-human IgG fixed to the membrane, adding an enzyme-labeled mycoplasma pneumoniae antigen solution; and adding a color developing solution for developing color and displaying a result at last. According to the method, the process of detecting the mycoplasma pneumoniae antibody by an enzyme-labeled mycoplasma pneumoniae antigen is quick and efficient, the quality guarantee period of the kit is prolonged, the detection sensitivity is improved, and the cost of the kit is reduced.

The invention provides a reagent device and a method used for detecting mycoplasma pneumonia antibodies. The reagent device has a long strip shape, and has a base body comprising 8 hole positions and a handle positioned on one end of the base body. From the end proximal to the handle, the 8 hole positions are sequentially a sample hole, an auxiliary agent hole, an enzyme conjugate hole, a substrate hole, a termination liquid hole, a dilution liquid hole, a reaction hole, and a dilution hole. According to the invention, based on a principle of enzyme-linked immunoassay, mycoplasma pneumonia antibody is detected by using the reagent device. The method is an independent single-person analysis and detection method. The device can be used in cooperation with a corresponding specific analysis instrument. During a detection process, detection reagents or samples are injected by using a full-automatic precise dosing device. The device and the method have the advantages of automatic operation, precise dosing, high detection result accuracy, high detection result precision, and wide application prospect.

The invention provides a mycoplasma pneumonia (MP) antigen. The antigen fragment of the antigen is MP371 or MP661, wherein the locus of MP 371 is at an N terminal 371-480aa of MP adhesion protein P1, and the locus of MP661 is at an N terminal 661-772aa of MP adhesion protein P1. The invention also relates to a kit comprising any antigen or antigen composition and the application of the kit in detection of a mycoplasma pneumonia antibody. The mycoplasma pneumonia antigen is a recombinant antigen cloned and expressed by a codon-optimized gene and has stronger antigen specificity than that of the antigen cultivated and extracted by MP. The kit can be used for specific detection of an anti-MP-IgM (immunoglobulin m) antibody in clinical samples, thus identifying and diagnosing the infection of mycoplasma pneumonia at early stage.

The invention provides a combinant for detecting a mycoplasma antibody and application of the combinant, and belongs to the technical field of biology. The combinant comprises seven polypeptides, wherein amino acid sequences of the polypeptides are shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7 respectively. The combinant can be used for specifically detecting the mycoplasma antibody of a pig nose, and the cross reaction on the mycoplasma antibody and other mycoplasmas or other pathogenic infection samples which are commonly seen on the pig body is avoided, and the false positive rate is extremely low. Detection samples are easily available, and the normal feeding management of a pig farm cannot be influenced.

The invention discloses an ELISA kit for rapid detection of Mycoplasma sheep pneumoniae antibody, comprising a coated enzyme labeling plate, negative standard serum, positive standard serum, enzyme labelled secondary antibody, washing solution, diluent, substrate solution and stop solution. By constructing an expression vector, a highly immunogenic recombinant Mycoplasma ovine pneumonia outer membrane protein MOL was obtained, which was used as a coating antigen, and a specific and highly sensitive ELISA detection method and kit for Mycoplasma ovine pneumoniae antibodies were established. The cassette is used for the detection of clinical samples of sheep serum, which will help to carry out in-depth research on the immune prevention and immune value of M. ovine pneumonia, and provide the necessary technical means for the rapid detection of M. ovine pneumonia antibodies.

The invention relates to the diagnostic medicine of animals, especially relates to a mycoplasma bovis diagnosis technology, and concretely relates to a multi-epitope fusion antigen having an amino acid sequence represented by SEQ ID NO:1 or SEQ ID NO:2, and its application in the preparation of a mycoplasma bovis diagnosis reagent. The diagnosis reagent can be used as a solid phase vector coating antigen of an indirect ELISA kit and is combined with its specific antibody, a horseradish peroxidase coupled anti-cattle IgG antibody is added and incubated, and a color development reaction is carried out, and the color development degree is proportional to the amount of the anti-mycoplasma bovis antibody in a sample to be measured. The technology has the advantages of simple operation, no need of complex equipment, low technical requirements on the laboratorial conditions and experiment personals, low detection cost, and suitableness for the large-scale development in the basic level and the culture farm; the multi-epitope fusion antigen has a low making cost and is suitable for large-scale application; and has the advantages of high sensitivity and specificity, small batch difference, and high detection result consistence because of the adoption of multi-epitope as a target.