A kind of mycoplasma bovis detection test strip and preparation method thereof
A technology of mycoplasma bovis and test strips, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of scarcity, weak sensitivity, and low sensitivity, and achieve a wide range of applications, stable markers, and strong specificity Effect
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[0020] The present invention is illustrated below in conjunction with examples.
[0021] 1. Expression and purification of P48 protein
[0022] 1.1 Sequence synthesis and recombinant plasmid construction
[0023] Mycoplasma bovis p48 gene (Genebank: NC_014760.1), the present invention optimizes the codons of this sequence, and optimizes the codons according to the preference of codons in Escherichia coli. TGA (UGA) was optimized to TGG (UGG), because this codon is a stop codon in Escherichia coli, and restriction sites BamHI and XhoI were added at both ends of it, and its gene sequence is SEQID№1;
[0024] The optimized p48 gene sequence was synthesized into the pet32a expression vector plasmid by Invitrogen Company. After the synthesis, it was identified by sequencing and enzyme digestion, and the positive recombinant plasmid after successful identification was named Pet32-a(+)-p48.
[0025] 1.2 Expression of P48 protein
[0026]Transform the recombinant plasmid pet32a(+)-...
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