Mycoplasma gallisepticum antibody detection reagent, preparation method and application thereof

Abstract

The invention relates to the technical field of poultry antibody detection reagents, particularly to a mycoplasma gallisepticum antibody detection reagent, a preparation method and application thereof. According to the invention, a mycoplasma gallisepticum culture solution is subjected to a series of treatment on, inactivating is performed, the inactivated antigen bacteria solution and a freeze-drying protective agent are uniformly mixed, and freeze drying is performed to obtain the mycoplasma gallisepticum antibody detection reagent; the reagent can be directly used for a hemagglutination inhibition test; a plate agglutination test can be carried out by adding any one coloring agent of methyl violet, amber red and crystal violet into the reagent containing 4-8HA unit of antigen; the hemagglutination value of the detection reagent reaches 26; and the detection reagent not only improves the hemagglutination titer of the mycoplasma gallisepticum antigen, but also has two purposes, is stable, sensitive, strong in specificity, long in storage life, simple to operate, convenient and fast, does not need special equipment and instruments, and can be used for efficacy test, epidemiologicalinvestigation, clinical sample detection and the like of mycoplasma gallisepticum vaccines.

Description

technical field [0001] The invention relates to the field of poultry antibody detection reagents, in particular to a detection reagent for mycoplasma gallisepticum antibodies. Background technique [0002] Mycoplasma gallisepticum is one of the most common pathogens in poultry. After chickens are infected with the disease, the main symptoms are cough, runny nose, rales when breathing, and mouth breathing in severe cases. The disease can hinder the growth of broiler chickens and reduce feed remuneration. , laying hens egg production decreased, breeder hatching rate is low. The disease presents a slow epidemic, and the resulting immunosuppression can easily cause secondary or concomitant infections of other diseases. In recent years, with the improvement of the intensification of my country's breeding industry, the prevalence of Mycoplasma gallisepticum has become more and more violent. cause huge economic losses. [0003] The pathogenic strain of mycoplasma has the biologica...

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Problems solved by technology

[0004] At present, the diagnostic technology used for this disease is mainly enzyme-linked immunosorbent assay (ELISA). Although the application of this method is mature, the operation time is long, the process is cumbersome, and the cost is high.

In China, only the China Veterinary Drug Administration sells Mycoplasma gallisepticum tiger red serum plate agglutination test antigen. The results are clear and easy to observe, simple to operate, fast and sensitive, and good repeatability. However, compared with the HI method (hemagglutination inhibition method), this method has Poor specificity, high sensitivity, can be used as a preliminary screening test for serological diagnosis

[0005] Because Mycoplasma gallisepticum is difficult to cultivate, although it has hemagglutination, the actual operation shows that the hemagglutination value is low and the sensitivity is low, so it cannot be applied to actual production. Therefore, it is particularly important to prepare highly sensitive hemagglutination antigens.

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