Method for multistep quantitatively detecting serum albumin and metaglobulin in plasma

Abstract

The invention discloses a method for multistep quantitatively detecting serum albumin and metaglobulin in plasma, belonging to the field of bioluminescence marking. The method comprises the following steps: adding fluorescent reagents in different serum albumin concentrations into the fluorescent reagent at several times and measuring DP-TPPNa fluorescence intensity after dropwise adding each time; acquiring the optimal concentration and the detection scope of DP-TPPNa for serum albumin according to a measuring result; dropwise adding the serum albumin and metaglobulin in different mass ratios into the fluorescent reagent at several times and measuring DP-TPPNa fluorescence intensity after dropwise adding each time; drawing a change curve of change rate of DP-TPPNa fluorescence intensity following the concentration of serum albumin according to the measuring result; selecting a standard curve according to the change curve; adding a plasma sample into the fluorescent reagent, measuring the DP-TPPNa fluorescence intensity, substituting into a linear equation of the standard curve and calculating the concentration of the serum albumin and metaglobulin.

Description

technical field [0001] The invention relates to a stepwise method for quantitatively detecting serum albumin and fibrinogen in plasma, in particular to a stepwise light-up method for quantitatively detecting serum albumin and fibrinogen in plasma, which belongs to the field of bioluminescence labeling. Background technique [0002] As a major protein in human plasma, serum albumin accounts for 50% of the total plasma protein. For a normal person, 10-15 grams of serum albumin is synthesized in the liver every day, and 40% of the serum albumin is released into blood vessels through the exchange of blood and interstitium. Serum albumin contains a polypeptide chain with 585 amino acid residues, a tryptophan residue, and a cysteine ​​sulfhydryl free radical, and its half-life in plasma is 15-19 days. Its secondary structure consists of three domains, each containing two subdomains. Three 2D domains form a heart shape, and slight pH changes reveal the flexibility of the central ...

AI Technical Summary

Problems solved by technology

The acquisition of serum requires blood to undergo a series of complex chemical purifications, which cannot be accomplished in ordinary hospitals, small clinics, or under the conditions of lack of medical equipment, and cannot truly achieve the simple detection purpose that does not require separation and purification.

In addition, the main difference between serum and plasma is that serum does not contain fibrinogen, and fibrinogen plays an irreplaceable role in plasma as a hemostatic protein. While accurately detecting serum albumin content in situ in plasma with fluorescent reagents, research serum The interaction between albumin and fibrinogen has not been reported in the literature

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