The invention relates to the field of edible and medicinal
processing, and in particular relates to an extraction method and application of
usnea longissima
polysaccharide. The extraction method comprises the following steps: by using
usnea longissima as a
raw material, performing
grinding, performing
degreasing, performing
drying to obtain
powder, mixing the
powder and water to form a dispersionliquid, adding
cellulase into the dispersion liquid, performing enzymolysis and performing heating extraction, separating filter residues, and concentrating the supernatant to obtain a concentrated solution; mixing the concentrated solution and an
alcohol, performing
alcohol precipitation, performing filtering to separate the precipitate, dissolving the precipitate into water, performing
centrifugation, performing
dialysis desalting, and performing
freeze drying on the product obtained after
dialysis desalting to obtain crude
sugar; and performing deproteinization treatment on the crude sugarby using a Sevage method to obtain a deproteinized solution, performing adsorption on
polysaccharide in the deproteinized solution by using an
ion exchange column, performing
elution on the
polysaccharide, after the
elution is completed, performing concentration, performing
dialysis desalting, and performing
freeze drying to obtain the
usnea longissima polysaccharide. The method can extract the high-purity usnea longissima polysaccharide; and the obtained product can be directly used for activating
intestinal immunity.