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Bacillus mucilaginosus bacterial fertilizer for farm onsite fermentation and applications thereof

A technology of jelly-like spores and bacilli, which is applied in applications, methods based on microorganisms, agriculture, etc., can solve the problems of being easily infected with E. coli, affecting the germination of spores, and has not been disclosed, so as to meet the requirements of facilities, which are simple, easy to implement, and increase The effect of bacteria content

Inactive Publication Date: 2016-03-30
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The second is that the early stage of spore germination takes a long time, usually 4 hours before the spores will start to germinate. At this time, due to the large-scale reproduction of yeast and lactic acid bacteria in the "activation" solution, the pH drops rapidly, which can already inhibit the germination and reproduction of spores. , thus greatly affecting the germination of spores
[0009] The third is that the high-nutrient medium is also susceptible to infection of Escherichia coli, the original plant pathogenic bacteria and other harmful bacteria, resulting in poor effects after application and still have side effects
[0010] However, there has not been disclosed so far a jelly-like bacillus fertilizer that can better control miscellaneous bacteria and promote rapid germination of spores and is applied to on-site fermentation and propagation of farms.

Method used

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  • Bacillus mucilaginosus bacterial fertilizer for farm onsite fermentation and applications thereof
  • Bacillus mucilaginosus bacterial fertilizer for farm onsite fermentation and applications thereof
  • Bacillus mucilaginosus bacterial fertilizer for farm onsite fermentation and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A jelly-like bacillus bacterium fertilizer used for on-site fermentation in a farm. The raw materials for preparing the jelly-like bacillus bacterium fertilizer are in parts by weight: 100 parts of jelly-like bacillus bacteria powder, 22 parts of colistin sulfate, sodium 2 parts of tamycin, 18 parts of methionine, 13 parts of leucine, 100 parts of corn flour, 150 parts of soybean meal powder, 30 parts of fructose, 2 parts of disodium hydrogen phosphate, 1 part of potassium chloride, 62 parts of brown sugar;

[0042] Wherein, the preparation method of the jelly-like bacillus bacterium fertilizer for on-site fermentation of the farm comprises the following steps:

[0043] , Preparation of jelly-like bacillus bacteria powder

[0044] Take out the preservation tube of the jelly-like Bacillus strain, use the slant medium of the test tube to scratch the slant for recovery, and culture at 30°C for 3-5 days. The slant bacteria were inoculated in the slant culture medium of t...

Embodiment 2

[0051] Application effect of fermented liquid fermented on-site by Bacillus jelly-like bacteria fertilizer on tobacco

[0052] . Test site: a farmer's planting site in Shizi Village, Tantou Town, Longhui County, Hunan Province

[0053] . Test method: Flue-cured tobacco variety: Zhongyan 99-10-1. When transplanting flue-cured tobacco seedlings, use 500 times the fermented liquid of fermented jelly-like Bacillus bacteria fertilizer to irrigate the roots once; Spray the fermentation broth of Bacillus bacteria fertilizer 250 times for the first time, and then spray the second and third times continuously every 20 days (a total of 4 kg of fermentation broth of fermented jelly-like Bacillus bacteria fertilizer per mu) . The control area was sprayed with the same amount of clear water while spraying with 250 times of bacterial fertilizer fermentation liquid in each test area;

[0054] . test results:

[0055] After applying the fermented jelly-like Bacillus fertilizer, th...

Embodiment 3

[0059] A kind of jelly-like bacillus bacterium fertilizer that is used for on-site fermentation on the farm, the raw materials for preparing the jelly-like bacillus bacterium fertilizer are by weight: 80 parts of jelly-like bacillus bacteria powder, 24 parts of colistin sulfate, sodium 1 part of tamycin, 22 parts of methionine, 14 parts of leucine, 1100 parts of corn flour, 150 parts of soybean meal powder, 35 parts of fructose, 2 parts of disodium hydrogen phosphate, 2 parts of potassium chloride, 62 parts of brown sugar;

[0060] Wherein, the preparation method of the jelly-like bacillus bacterium fertilizer for on-site fermentation of the farm comprises the following steps:

[0061] , Preparation of jelly-like bacillus bacteria powder

[0062] Take out the preservation tube of the jelly-like Bacillus strain, use the slant medium of the test tube to scratch the slant for recovery, and culture at 30°C for 3-5 days. The slant bacteria were inoculated in the slant culture me...

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Abstract

The invention relates to a bacillus mucilaginosus bacterial fertilizer for farm onsite fermentation, a preparation method thereof and applications thereof. The bacterial fertilizer comprises the following raw materials: bacillus mucilaginosus powder, colistin sulfate, natamycin, methionine, leucine, corn flour, soybean cake powder, fructose, disodium hydrogen phosphate, potassium chloride, and brown sugar. The colistin sulfate and natamycin in the bacterial fertilizer can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (leucine, fructose, and methionine) can rapidly promote the germination of spores of bacillus mucilaginosus and improve the germination rate of spores. The provided bacterial fertilizer can be applied to the onsite fermentation and propagation in a simple and crude farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content, good activity and few contaminating bacteria. The bacterial fertilizer can decompose potassium, prevent disease, promote growth, increase output, and improve product quality. The effect is stable.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a jelly-like bacillus bacterial fertilizer applied to on-site fermentation in farms and a preparation method and application method thereof. Background technique [0002] The average content of potassium in the earth's crust is 2.6%, and its abundance ranks fourth among all elements contained in the earth's crust. The soil potassium content is generally between 0.04-3.0%. According to the existing form, it is divided into water-soluble potassium, exchangeable potassium, non-exchangeable potassium and mineral potassium. The content and exchange of each form of potassium determine the potassium supply capacity of the soil. 90% of the potassium in the soil exists in the crystal structure of potassium-containing minerals such as mica and feldspar, and can only be absorbed and utilized by crops after being released from the crystal structure after a long weathering process. [00...

Claims

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Application Information

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IPC IPC(8): C05G3/00C12N1/20C12R1/07
CPCC05G3/00C05B7/00C12N1/20
Inventor 胡艳晖其他发明人请求不公开姓名
Owner FOSHAN YANHUI BIOTECH CO LTD
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