The invention relates to a
pathological tissue section staining kit, which belongs to the field of
pathological tissue section staining and comprises a section dewaxing solution, a conversion solution, a cleaning solution, a differentiation solution, a blue returning solution, a hematoxylin
staining solution, an
eosin staining solution and a mounting medium, the invention provides the novel
pathological tissue section staining kit which is simple in component, good in effect and low in
toxicity, and the preparation method, and the kit is mainly used for pathological tissue H&E staining. The method is characterized in that 1, the harm of traditional
dyeing reagents such as dimethylbenzene and
alcohol to an operator is reduced; 2, the
pollution to the environment is reduced; 3, extra deionized water does not need to be introduced in the
dyeing process; 4, a differentiation solution and a blue returning solution are designed by adopting a buffer
system, so that the effect is milder, and the coloring effect of H&E
dyeing is clearer and brighter; and 5, compared with a traditional hematoxylin formula, beta-
cyclodextrin is introduced into the kit to neutralize a product
iodine reduced by
sodium iodate, so that the dyeing capability of the staining solution is improved, and meanwhile, a
reducing agent dibutylated hydroxytoluene is introduced, so that the service life and the
shelf life of the staining solution are prolonged.