H-E dyeing liquid for pathological tissue dyeing machine

Abstract

The invention provides an H-E dyeing liquid for a pathological tissue dyeing machine, and relates to the technical field of biological preparations. The H-E dyeing liquid comprises modified hematoxylin dyeing liquid and modified eosin dyeing liquid, wherein the modified hematoxylin dyeing liquid comprises following components: sodium iodate, hematoxylin, aluminum potassium sulfate, anhydrous ethanol, formaldehyde, glacial acetic acid, and water; and the modified eosin dyeing liquid comprises following components: alcohol-soluble eosin, anhydrous ethanol, aluminum potassium sulfate, sodium iodate, water-soluble eosin, erythrosine, glacial acetic acid, formaldehyde, and deionized water. The using cost is reduced, the sample preparation time is shortened, the provided H-E dyeing liquid can evenly dye pathological tissues under the assistance of a pathological tissue dyeing machine, the structure of dyed pathological tissues is clear, the color of cells is bright, and the observation on slides of pathological tissues becomes easy in clinic.

Description

technical field [0001] The invention relates to the technical field of biological preparations; in particular, it relates to an H-E staining solution used in a pathological tissue staining machine. Background technique [0002] At present, the incidence of serious diseases such as various cancers, tumors, and tissue lesions is on the rise. How to effectively diagnose these diseases has gradually become a hot spot in pathological research. [0003] Pathological tissue preparation technology can assist clinicians to detect the above-mentioned diseases early and formulate correct treatment plans, so that patients can recover as soon as possible and reduce pain, so it has attracted the attention of researchers and clinicians. [0004] One of the important steps in pathological tissue preparation technology is to stain the pathological tissue, and H-E staining solution is one of the most commonly used staining methods. The sample cells stained with H-E staining solution have goo...

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Problems solved by technology

[0008] The problem to be solved by the present invention is that the preparation of the traditional H-E staining solution is relatively complicated, the staining solution is easy to be old, and the dyeing effect is unstable, and the effect is not good when used with a highly automated pathological tissue staining machine, which affects the accuracy of the doctor's pathological reading. , affecting the diagnosis of the disease