Pathological section staining composition of ovarian cancer

Abstract

The invention relates to a pathological section staining composition of ovarian cancer. The composition is prepared from hematoxylin, absolute ethyl alcohol, aluminium potassium sulfate, sodium iodate, glacial acetic acid, ferrous sulfate, ferrous sulfate chitosan PLGA double microspheres and distilled water. According to the composition, the components of the hematoxylin staining fluid are simple, the cost is low, the staining fluid is safe and effective and has no toxic and side effects on the human body, in the preparation process, there are no strict requirements on the dosage of oxidizingagent and the oxidation time, the difficulty of preparation is effectively reduced, the consumed time for preparation is obviously shortened, after preparation of the staining fluid is completed, thestaining ability can be maintained for a long time, no oxidation film and precipitation can be formed, the validity period of the hematoxylin staining fluid is greatly prolonged, the staining composition has an excellent staining ability for various pathological tissues, especially, the staining effect on the ovarian cancer is better, and nuclei, chromatin, nuclear membranes and nucleoli can be stained clearly.

Description

technical field [0001] The invention belongs to the technical field of diagnostics, and in particular relates to a pathological slice staining composition for ovarian cancer. Background technique [0002] In clinical practice, some diseased tissues or organs are processed to fix and harden, then sectioned and stained, and the pathological changes are observed under a microscope to make a pathological diagnosis. The classic staining steps include hematoxylin-eosin staining, HE staining method for short, is to use alkaline hematoxylin staining solution to stain the chromatin in the nucleus and the nucleic acid in the cytoplasm into purple blue, and use acidic eosin to stain the components in the cytoplasm and extracellular matrix into red . [0003] Hematoxylin, one of the dyes in the hematoxylin-eosin dyeing method, is a pure natural dye extracted from the hematoxylin tree that is mainly produced in Mexico. It is one of the most commonly used dyes and can be used for silk an...

AI Technical Summary

Problems solved by technology

[0004] Naturally matured hematoxylin requires a long cycle, high cost, and troublesome use, while artificially matured hematoxylin, in addition to the excessive oxidation of hematoxylin that causes its dyeing power to rapidly weaken or even disappear, mercury oxide and other substances have toxic side effects, Oxide film and precipitation will also be formed on the surface of the dye solution and the container wall, resulting in a rapid decline in the dyeing ability, short validity period, and cannot be stored for a long time.

Aiming at the problems of artificially matured hematoxylin, a semi-oxidized hematoxylin has been developed. The principle is to oxidize most of the hematoxylin to oxidized hematoxylin in a short period of time, so that it has the ability to dye, and the rest Part of the hematoxylin is in a semi-oxidized state, and it is naturally oxidized during continued use, thus effectively preventing the overoxidation of oxidized hematoxylin. Through natural oxidation, the consumed pigment can be continuously replenished to maintain the relative stability of the dye solution and relatively durable dyeing ability, but the half-oxidized hematoxylin dye solution reduces the peroxidation of oxidized hematoxylin at the cost of sacrificing part of the dyeing ability at the beginning of preparation, artificially reducing the dyeing ability of the staining solution, and The preparation method is complicated, and it is necessary to choose the appropriate amount of oxidant and oxidation time according to the amount of dyeing liquor. When the hematoxylin in the semi-oxidized state is naturally matured for about 3 months, it still faces the dyeing ability caused by the peroxidation of oxidized hematoxylin. The problem of falling too fast, the validity period is short, and it cannot be stored for a long time

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