67 results about "Protein combining" patented technology

The present invention relates to biocompatible protein particles, particle devices and their methods of preparation and use. More specifically the present invention relates protein particles and devices derived from such particles comprising one or more biocompatible purified proteins combined with one or more biocompatible solvents. In various embodiments of the present invention the protein particles may also include one or more pharmacologically active agents and / or one or more additives.

The present invention relates to mucoadhesive drug delivery devices and their methods of preparation and use. More specifically the present invention relates to mucoadhesive drug delivery devices comprising one or more biocompatible purified proteins combined with one or more biocompatible solvents and one or more mucoadhesive agents. The mucoadhesive drug delivery devices may also include one or more pharmacologically active agents. The drug delivery devices of the present invention adhere to mucosal tissue, thereby providing a vehicle for delivery of the pharmacologically active agent(s) through such tissue.

The present invention relates to mucoadhesive drug delivery devices and their methods of preparation and use. More specifically the present invention relates to mucoadhesive drug delivery devices comprising one or more biocompatible purified proteins combined with one or more biocompatible solvents and one or more mucoadhesive agents. The mucoadhesive drug delivery devices may also include one or more pharmacologically active agents. The drug delivery devices of the present invention adhere to mucosal tissue, thereby providing a vehicle for delivery of the pharmacologically active agent(s) through such tissue.

The present invention relates to biocompatible protein-based particles and their methods of preparation and use. More specifically the present invention relates protein-based particles including protein matrix, spread matrix and / or biocoacervate materials derived from one or more biocompatible purified proteins combined with one or more biocompatible solvents that are used to replace or repair tissue and / or bone in treatments for spinal disc(s), joint(s) (e.g. knee, hip, finger, ankle, elbow, shoulder . . . ) and organ(s) (e.g. bladder, lips, vagina, penis, urethra . . . ). In various embodiments of the present invention the protein-based particles may also include one or more pharmacologically active agents and / or one or more additives.

The invention relates to fusion protein of SARS-Cov virus structural protein and its high expression and purification in mammalian cell and its usage. The construction of said fusion protein is X-Y-z, and X is S or M or E or N selected from SARS-CoV virus construction protein, or their arbitrary short form; Y is the connection part with 0-20 amino acid; Z is Fc and its modification or other protein tag. The invention also provides the method of expressing and purifying said fusion protein in mammalian cell for the batch preparation or industrial production. The fusion protein can be used to prepare genetic engineering vaccine preventing SARS-CoV virus infection, solvent box for checking SARS-CoV virus, and to sift drug anti SARS-CoV virus infection with S protein combined with its acceptor ACE2. The invention can detect combination of S protein of SARS-CoV virus with ACE2, which reduces ACE2 expression, and result in or exacerbate acute lung damage, then it modifies combined segment, which can improve safety of preventing SARS-CoV virus vaccine.

The invention discloses a polypeptide specifically combined with a HepG2 cell surface. According to the invention, four polypeptide segments are selected by utilizing a phage display random dodecapeptide library, the amino acid sequences of the four polypeptide segments are respectively LLADTTHHRPWT, LLADTPHHRPWT, FGWVTPHHELRS and SLSDLTHMGPWP. According to the invention, a polypeptide sequence combined with a liver cancer HepG2 cell is selected by utilizing a phage polypeptide display technology, and ELISA (enzyme-linked immunosorbent assay) identifies the affinity of phage clone and the liver caner cell, thus eight phage clones are obtained; four polypeptide sequences are obtained by sequencing, wherein the common amino acid sequence (basic sequence) is ***D(V)TT(P)HH*P(L)W(R)*; homology analysis indicates that the basic sequence of the polypeptide is possibly amino acid determinant on a ligand protein combined with a tumor cell surface receptor; cell immunofluorescence further identities that the target result of the positive clone of the phage prompts that the positive clone of the phage can be specifically combined with the HepG2 cell; and the selected specific polypeptide of the liver cancer HepG2 cell provides an experiment basis for early diagnosis of liver cancer, targeting delivery of an antitumor medicine and research and development of a targeting short peptide medicine.

The invention discloses a multiple organ function support system, formed by a body, an external blood circuit, a plasma separating-adsorbing circuit, an albumin circuit, a dialysate circuit, a feeding circuit and an operating system. The invention can eliminate the inflammation medium, toxin and lots of middle and small molecule materials in the body of multiple organ function obstacle complex symptom (MODS) patient, and uses a lung membrane oxygen generator to replace the air exchange function of lung, to be used in reversal breath exhaustion, and uses albumin circulate purification treatment to completely eliminate the protein combine toxin and soluble small molecule toxin (as blood ammonia) of lung exhaustion patient, and accurately release water slowly. The invention can combine the support functions of lung, heart, kidney and liver, based on present clinic test and technique, with wide application.

The invention discloses a method for extracting crude fish oil in leftovers of tilapia. The method includes the following steps that at first, the leftovers of the processed tilapia are cleaned and minced after gall bladders are cleared out, and put into an enzymatic vessel, and then Alcalase enzyme and trypsinase are added step by step into the enzymatic vessel for enzymolysis; after the enzymolysis, the enzymatic hydrolysate is centrifuged to produce crude fish oil I; the centrifuged emulsion is frozen, thawed, demulsified, and centrifuged for the second time to produce crude fish oil II. The invention decomposes the protein of the tilapia leftovers by protease, releases fat from protein and degrades the protein combining emulsion with fat and produced in the enzymolysis process; the invention is also combined with the freezing, thawing and emulsion breaking technology, improving the crude fish oil extraction rate of tilapia and the added value of the product.

The invention provides a protein in situ expression chip and a preparation method and an application thereof. The chip comprises a substrate fixed with a capture carrier and a microfluidic template arranged on the substrate, wherein at least one protein combined with the capture carrier is also distributed on the substrate, and the protein is obtained by in situ expression on the chip; a flow cell inlet and a flow cell outlet are respectively formed in the two ends of a flow cell. According to the invention, the protein in situ expression chip is constructed to be not only applied in traditional fluorescence detection, but more importantly applied on a biological sensor for monitoring the interaction of a kinetic process in real time; and moreover, the protein in situ expression chip can be regenerated and recycled through certain regeneration buffer solution.

The invention belongs to the technical field of biological medicine, and discloses protein combined nanometer selenium, and a preparation method and applications thereof. According to the preparationmethod, under the reducing action of a disulfide bond reducing agent, the internal space structure of a disulfide bond containing protein or a disulfide bond containing polypeptide is opened so as toobtain mercapto groups with reducing activity, and a part of the mercapto groups are used for converting selenium compounds into elemental selenium through reducing, the rest mercapto groups are usedfor forming intramolecular and intermolecular disulfide bonds, so that self assembling of protein combined nanometer selenium is obtained; the preparation method is capable of improving the dispersion performance and the stability of nanometer elemental selenium in solution greatly. It is confirmed by animal experiments that the active red elemental selenium is high in bioavailability and low intoxicity. The protein combined nanometer elemental selenium possesses the functions of protein and nanometer selenium, is small in nanometer particle size, is narrow in distribution range, can be stored in the state of liquid phase, is convenient for oral administration, injection, or spraying.

The invention relates to the technical field of biology, in particular to a method for extracting mciro-RNA from plasma and a kit thereof. The method comprises the steps that a lysate is added into a plasma sample, and a protein removal solution is added; an RNA extraction reagent is added for performing RNA extraction, then, chloroform is added for oscillation mixing, and centrifuging is performed to collect supernatant liquid; a precipitating agent and a settling agent are added, and centrifuging is performed to collect sediments; a washing solution is used for performing washing, centrifuging is performed to collect sediments, RNase-free water is used for performing dissolution. According to the extraction method, firstly, sample cracking and protein removal are performed, and then RNA extraction is performed, so that proteins combined with the micro-RNA are modified to release the micro-RNA, and the integrity of the micro-RNA is kept. According to the kit, the lysate and the protein removal solution are used at the same time for helping sufficient degradation of proteins combined with the micro-RNA, and the precipitant isopropanol and the settling agent glycogen are used at the same time, so that the micro-RNA recycling efficiency is greatly improved.

The invention relates to methods of identifying a binding partner of a target molecule within a plurality of analyte molecules, including a plurality of peptides and / or proteins. The target molecule is physically combined with a target labeling nucleic acid molecule. Each member of the plurality of analyte molecules is physically linked to an analyte labeling nucleic acid molecule, each analyte labeling nucleic acid molecule comprising a selected nucleotide sequence. This specific nucleotide sequence may include a sequence encoding a peptide / protein combined therewith. The target molecule is contacted with the analyte molecules and a complex between the target molecule and an analyte molecule forms. The mixture is subdivided into compartments. The target labeling nucleic acid molecule and the analyte labeling nucleic acid molecule are linked and the plurality of compartments allowed to disintegrate. The linked nucleic acid molecule is retrieved and the sequence determined.

The invention discloses a bovine A-type foot-and-mouth disease broad-spectrum multi-epitope recombinant vaccine, and a preparation method and an application thereof, and belongs to the field of veterinary vaccine research. The preparation method comprises the following steps: carrying out reasonable serial connection on the major antigen epitopes of pandemic in China and bovine A-type foot-and-mouth disease virus representative strains recently pandemic in countries bordering China by adopting a brand new reverse vaccinology idea and strategy, coupling with a bovine IgG heavy chain constant region, cloning into a prokaryotic expression vector to construct a recombinant expression vector, transforming Escherichia coli cells to express a recombinant antigen, purifying by adopting Ni-NAT column chromatography, quantifying by a Bio-Rad protein quantification kit, and preparing the vaccine individually or combining with a recombinant foot-and-mouth disease virus 3D protein. A result of animal immune experiments shows that the multi-epitope antigen or 3D protein combined vaccine can stimulate the body to produce a protective antibody with high titer and also can protect immune animals against a virus attack, so the bovine A-type foot-and-mouth disease broad-spectrum multi-epitope recombinant vaccine has a good application prospect.

The invention relates to the technical field of biologic and especially relates to a "polypeptide-protein combined-type marker" detection kit relative to colorectal cancer. The kit contains a capture antibody and a detection antibody, wherein the capture antibody is specifically combined to protein HKa and the detection antibody is specifically combined to polypeptide HKP15 or polypeptide HKP09. To a HKP09 / HKP15-HKa combined-type marker, the kit has a linear detection range being 6-120 ng / ml and a minimum detection limit being 1.7 ng / ml. To distinguish a colorectal cancer sample from a normal human sample, the kit is 93.7% in sensitivity and is 92.7% in specificity. The novel combined marker detected in the kit provided in the invention is closely relative to the colorectal cancer. In addition, a detection technology is very high in accuracy and sensitivity, is simple and quick in operation, is beneficial to high-flux clinical detection and can reduce a detection cost.

The invention provides an acinetobacter baumannii and subunit protein combined vaccine and a preparation method thereof. According to the preparation method, acinetobacter baumannii SmpA (small protein A) represented as SEQ ID No.1 and an acinetobacter baumannii PLD (phospholipase D) nucleotide sequence represented as SEQ ID No.2 are connected to expression vectors respectively, SmpA and PLD recombinant plasmids are established respectively, recombinant SmpA and PLD proteins are subjected to induced expression respectively, and then the two proteins are mixed with adjuvants for preparation of the vaccine. Based on immunoinformatics and reverse genetics, antigenicity and immunogenicity of outer membrane protein components of the multi-drug-resistant acinetobacter baumannii are analyzed, gene cloning, protein expression, purification and immunological research are performed, the effective outer membrane protein components are screened out and combined, the safety and the effectiveness of the vaccine are improved, and the vaccine has more realistic and more attractive application prospect compared with conventional vaccines.

The invention relates to the field of biochemistry, and relates to an immobilized compound enzyme and a method for extracting garlic polysaccharide from an alliin waste liquid by using the immobilized compound enzyme. According to the method, broken kernel enzyme, namely pectinase (enzyme activity is 100,000 u / g) and protein removing enzyme, namely bromelain (enzyme activity is 100,000 u / g) are fixed in an epoxy macroporous resin, the broken kernel enzyme can hydrolyze cellulose to break cell walls, protein polysaccharide is released, and the protein removing enzyme can perform enzymolysis on protein combined with polysaccharide. The immobilized compound enzyme is adopted for extracting the garlic polysaccharide from the alliin waste liquid, a garlic polysaccharide product is prepared by steps of filtration, 1.5-5mu m ultrafiltration membrane separation, concentration and spray drying; and by the method, the yield of the polysaccharide can be improved, the content of protein in the polysaccharide is reduced and the purity of the polysaccharide is improved.

The invention relates to an external molecule adsorption cycle system, which comprises an external blood cycle tube, and an albumin liquid cycle tube, wherein the first tube comprises a blood pump and an intelligent adialyser; the intelligent dialyser is arranged with the analogue film with the selectivity on the protein combine toxin and the soluble toxin; the analogue film is the hollow fiber film whose wall thickness is 10-30micrometers, the total surface is 2.1-2.4m2, and the aperture of the hole on the film can allow passing the molecule whose molecule weight is less than 60000-66000. The invention has the advantages that: 1, the wall thickness is controlled, to reduce the broken rate; 2, the aperture is controlled to reduce the treat time; 3, the dosage of anticoagulant is reduced to avoid leaking blood; 4, the surface is controlled by reasonable math model with high film separate property.

The invention discloses a high-efficiency soybean meal protein extraction device and method. The extraction device mainly comprises: a tank body A and a tank B which are arranged in parallel; a centrifuge A and a centrifuge B which are symmetrically arranged at the upper ends of the tank body A and the tank body B correspondingly; and a post-treatment tank arranged at the side edges of the tank body A and the tank body B. One part of the high-efficiency soybean meal protein extraction device is used for extraction, the other part is used for hydrolysis, and the two parts can be repeatedly used multiple times as demanded, so the protein extraction efficiency is improved, and the occupied area and the purchasing cost of the device are reduced. The high-efficiency soybean meal protein extraction method comprises two-time extraction and two-time hydrolysis, the extraction mainly uses an alkali dissolution technology, and the two-time extraction guarantees the protein extraction rate and reduces the waste of a liquid obtained after separation; and the hydrolysis is mainly used for separating a small amount of proteins combined with fats in soybean meal, so the protein extraction rate is effectively improved.

The invention relates to a placental chondroitin sulfate A based cancer screening and early stage diagnosis reagent, and a method, and more specifically discloses an ELISA kit used for tumor detection. The ELISA kit comprises a capturing protein combined with placental chondroitin sulfate A (pl-CSA), and the capturing protein is preferably selected from the minimum bonding peptide segments of plasmodium infected erythrocyte surface antigen (VAR2CSA, rVAR2), and more preferably, the sequence of the minimum bonding peptide segment is represented by SEQ ID No.1. It is found for the first time that pl-CSA can be detected in a plurality of kinds of body fluid, so that basis is provided for in vitro qualitative or quantitative biological detection of pl-CSA. The method is low in detection limit,excellent in repeatability, and high in specificity.

The invention relates to a method of extracting heparin sodium from mucous membrane of small intestines of pigs. The fresh mucous membrane of small intestines of pigs is adopted as a raw material. Adding amounts of materials are based on the using amount of the mucous membrane of the small intestines. The method includes: pretreating the fresh mucous membrane of the small intestines of the pigs, decomposing protein combined with heparin by an alkali extraction method and an enzymolysis method, centrifuging enzymatic hydrolysate to obtain a clear liquid, removing polypeptide, nucleic acid and other impurities in the enzymatic hydrolysate by adoption of the membrane technology by utilization of molecular weight differences of the heparin and other impurities, subjecting the liquid treated by the membrane to alcohol precipitation treatment by utilization of the characteristic that the heparin sodium is insoluble in ethanol, and drying the obtained precipitate to obtain a heparin sodium product. The method of extracting the heparin sodium fully utilizes poultry internal organ resources to produce a product with high added value, and is simple and feasible in process and suitable for industrial production. The tilter of the obtained heparin sodium is 150 u / mg. The molecular weight of the obtained heparin sodium ranges between 200 Dal and 1000 Dal.

The invention relates to an antitumor and thrombolytic double-effect chimeric protein, a preparation method thereof and the use thereof. The amino acid sequence of the chimeric protein is represented by an amino acid sequence SEQ ID No.4 or an amino acid sequence SEQ ID No.5. The preparation method of the chimeric protein comprises: constructing a chimeric gene; inserting the chimeric gene subjected to enzyme digestion into the EcoRI / XhoI locus of a carrier plasma Pet28a(+) to construct a recombinant plasma; transferring the recombinant plasma into the BL 21 competent cells of Escherichia coli to obtain recombinant Escherichia coli; culturing the recombinant Escherichia coli in a shake flask, disrupting the recombinant Escherichia coli by ultrasonic waves, passing collected supernate through a Ni-NTA affinity chromatographic column, performing gradient elution, condensation and desalination to obtain the chimeric protein. The chimeric protein combines antitumor and antithrombotic effects.

The invention belongs to the technical field of intestine casing membrane processing, and relates to a collagen-and-wheat-protein combined edible intestine casing membrane and a preparation method thereof. The preparation method comprises the following steps as follows: firstly, material processing, to be specific, wheat protein is dissolved; secondly, mixed processing, to be specific, a wheat protein solution obtained from the step (1) is fully mixed with collagen, the pH value of the mixed solution is adjusted within a certain range, then a certain amount of a plasticizer and a cross-linking agent is put into the solution, stirred sufficiently and uniformly so as to obtain a membrane forming liquid; thirdly, membrane forming and drying, to be specific, the membrane forming liquid is subjected to tape casting and membrane forming, and is dried at certain temperature for a certain period of time so as to obtain an intestine casing membrane semi-finished product; fourthly, curing processing, to be specific, curing processing is carried out at a certain temperature and humidity conditions, so as to obtain an intestine casing membrane finished product. According to the invention, the prepared protein composite intestine casing membrane has the advantages of high tensile strength, high breakage elongation rate, high nutritional value, and the like.

The invention relates to a process for purifying Fc fusion protein. The Fc fusion protein involved in the invention comprises but is not limited to recombinant human Fc fusion pegylated interferon (Fc-IFN). The process for purifying the Fc fusion protein combines the factors of the characteristics of Fc fusion protein, the requirements of purity and yield of purified samples, and the requirements of the amplification of the subsequent purification process and the preparation and formula and the like. A purification process which is suitable for industrial production is determined through laboratory research and scaled-up pilot test research. The process comprises the steps of centrifuging and pre-treatment, primary purification of ProteinA SepharoseFF chromatography and refined purification of Q-Sepharose FF and G25 Sephadex chromatography. The purity of the target protein obtained by purification reaches over 95 percent, the protein purification yield reaches over 40 percent, and the purity and yield of the target protein are improved. Research results prove that: the purification process is simple and convenient and suitable for industrial scale-up production, and the purity and the yield of the target protein are high.

The invention belongs to the field of biomedicine detection and particularly relates to a protein combined chip for Lyme disease immunoserology diagnosis and a preparation method and application thereof. The protein combined chip comprises a solid phase carrier and capture molecules fixed to the surface of the solid phase carrier, and the capture molecules comprise Borrelia burgdorferi flagellin, Borrelia burgdorferi outer membrane protein C (OspC) and Borrelia burgdorferi variable main protein sequence expression E protein (VlsE). The protein combined chip can further comprise capture molecules VlsE IR6 polypeptide. Compared with a traditional method, the protein combined chip can conveniently distinguish infection types on the basis of greatly increasing the positive rate.

The present invention relates to methods of identifying a binding partner of a target molecule within a plurality of analyte molecules, including a plurality of peptides and / or proteins. The target molecule is physically combined with a target labeling nucleic acid molecule, which includes a specific nucleotide sequence. Where the target molecule is a peptide / protein this specific nucleotide sequence may include a sequence encoding the target molecule. Each member of the plurality of analyte molecules is physically linked to an analyte labeling nucleic acid molecule, each analyte labeling nucleic acid molecule comprising a selected nucleotide sequence. This specific nucleotide sequence may include a sequence encoding a peptide / protein combined therewith. The target molecule is contacted with the analyte molecules and a complex between the target molecule and an analyte molecule forms. The mixture is subdivided into compartments, with each compartment comprising at most one target molecule or one complex between the target and an analyte molecule. The target labeling nucleic acid molecule and the analyte labeling nucleic acid molecule are linked and the plurality of compartments allowed to disintegrate. The linked nucleic acid molecule is retrieved and the sequence determined.

The invention relates to a DNA combine protein check method based on mobile cutting tangent enzyme, which designs a section of double stranded probe for the recognize sequence of an object DAN combine protein, wherein one single strand 5' end is marked with a connecting group 1 to be fixed on the surface of a solid carrier 2, and another single strand 5' end is marked with a signal molecule 6, the 3' end of the recognize point 3 with the object protein is arranged with a IIS type tangent enzyme recognize point 5, adjusts the distance between two recognize points to arrange the enzyme tangent pint 4 in the combine point 3. And the operation comprises that I, cultivating the solution with object DNA combine protein with the fixed double stranded probe, to process enzyme tangent reaction in a tangent enzyme system, II, when the object protein exists, the signal molecule is held on the surface of the carrier, and when the object protein does not exist, the probe is cut off by the tangent enzyme in the idle protein combine point, III, based on the signal strength, realizing the non-mark check on the object protein.

This invention discloses a magnetic card extractor for extracting DNA, RNA or protein. The magnetic card extractor comprises a test tube rack and a magnetic box. The both sides of the test tube rack are tightly locked on the magnetic box by clasps and the lock on the card, so that the test tube in the hole of the upside-down extractor cannot fall. DNA, RNA or protein combined with the magnetic reagent in the test tube is adsorbed to the test tube wall by the magnetic force, so that DNA, RNA or protein is left in the test tube after pouring the waste liquid in the experiment.

The invention aims at providing a reagent for detecting the interest protein expression condition in paraffin-embedded tissue. According to the technical scheme, a detection kit for evaluating prognosis information of an esophageal squamous carcinoma patient is provided. The kit comprises antibody primary antibody CD105 protein, esVEGFR2 protein and MYC protein antibodies for resisting three kinds of interest protein, and further comprises goat serum, 0.01M citrate restoration liquid, 3% H2O2, a Polymer enhancer, a Polymer, a DAB color reagent and a PBS solution. The kit can be used for evaluating prognosis of the esophageal squamous carcinoma patient, and is simpler than international TNM staging and easy to carry out, the sensibility and specificity of the kit are both higher than those of TNM staging, and the kit is more suitable for esophageal squamous carcinoma prognosis evaluation of Chinese.

The invention discloses a method for separating a membrane receptor protein. The protein combined with a preS1 peptide fragment of a hepatitis B virus on a cytomembrane of a hepatoma carcinoma cell is separated by virtue of immunomagnetic beads; and a method for rapidly separating the membrane receptor protein is built. According to the research result, the molecular weight range of a band is roughly equivalent to that of the foregoing scholar; the band is relatively abundant; the immunomagnetic beads are feasible for separating receptor associated protein; the overall process from preparation of the membrane protein to gel scanning can be finished within 2d; and the operation is very simple. Compared with other methods, according to the method disclosed by the invention, the effects of saving time and labor can be achieved, and the method is convenient and fast.