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CD105, esVEGR2 and MYC three-protein combined prediction esophageal squamous carcinoma patient prognosis kit

A technology of esophageal squamous cell carcinoma and a kit, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problem of not predicting the prognosis of patients with esophageal squamous cell carcinoma.

Inactive Publication Date: 2016-11-23
SHANTOU UNIV MEDICAL COLLEGE
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Problems solved by technology

However, there is currently no simple and effective method other than the international TNM staging that can more accurately predict the prognosis of patients with esophageal squamous cell carcinoma

Method used

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  • CD105, esVEGR2 and MYC three-protein combined prediction esophageal squamous carcinoma patient prognosis kit
  • CD105, esVEGR2 and MYC three-protein combined prediction esophageal squamous carcinoma patient prognosis kit
  • CD105, esVEGR2 and MYC three-protein combined prediction esophageal squamous carcinoma patient prognosis kit

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Experimental program
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Embodiment Construction

[0013] We collected archival wax blocks of surgical specimens from 124 patients with esophageal squamous cell carcinoma from 2000 to 2006. Further detailed description will be given below in conjunction with the embodiments of the accompanying drawings.

[0014] The steps of immunohistochemical hypersensitivity two-step method are as follows:

[0015] After the specimens were serially sectioned, dewaxed with xylene, and hydrated with graded alcohol, the expression of the target protein was detected by an ultrasensitive two-step method. The specific method is as follows:

[0016] (1) The slices were repaired with 0.01M citrate repair solution, 3% H 2 o 2 After inactivating endogenous peroxidase and blocking with goat serum, incubate with CD105, esVEGFR2 and MYC antibodies overnight at 4°C;

[0017] (2) After the slices were incubated with Polymer enhancer and Polymer polymer respectively, DAB color development;

[0018] (3) The sections were counterstained with hematoxylin...

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Abstract

The invention aims at providing a reagent for detecting the interest protein expression condition in paraffin-embedded tissue. According to the technical scheme, a detection kit for evaluating prognosis information of an esophageal squamous carcinoma patient is provided. The kit comprises antibody primary antibody CD105 protein, esVEGFR2 protein and MYC protein antibodies for resisting three kinds of interest protein, and further comprises goat serum, 0.01M citrate restoration liquid, 3% H2O2, a Polymer enhancer, a Polymer, a DAB color reagent and a PBS solution. The kit can be used for evaluating prognosis of the esophageal squamous carcinoma patient, and is simpler than international TNM staging and easy to carry out, the sensibility and specificity of the kit are both higher than those of TNM staging, and the kit is more suitable for esophageal squamous carcinoma prognosis evaluation of Chinese.

Description

technical field [0001] The invention relates to a kit for combining multiple protein antibodies and using an immunohistochemical hypersensitive two-step method to detect the expression of specimens from patients with esophageal squamous cell carcinoma, thereby predicting the prognosis of patients. Background technique [0002] Esophageal squamous cell carcinoma (ESCC), referred to as esophageal squamous cell carcinoma, is a common malignant tumor of the upper gastrointestinal tract in my country and ranks fourth in the mortality rate of malignant tumors in China. Of the more than 400,000 new cases of esophageal squamous cell carcinoma each year in the world, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal squamous cell carcinoma in the world. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in Henan Province, Yangquan in Shanxi Province, Yant...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57407
Inventor 许丽艳陈博许秀娥吴健谊李恩民
Owner SHANTOU UNIV MEDICAL COLLEGE
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