The invention discloses a method for preparing a temperature
stimuli responsive intelligent
chromatographic column, which comprises the following steps of: A, uniformly mixing N-
Isopropyl acrylamide, N,N'-
methylene-bis-
acrylamide,
polyethylene glycol-20,000, 10 percent w / v
ammonium persulfate and deionized water by using ultrasonic
waves; B, deoxidizing reaction products in the step A, adding 10 percent v / v N,N,N',N'-tetramethyl
ethylene diamine, and uniformly mixing fully; and C, pouring reaction products in the step B into a stainless steel
chromatographic column tube, performing reaction at the temperature of between -18 and 60 DEG C for 10 to 100 hours, arranging
chromatographic column heads at two ends of the column tube, connecting the column tube to a
high pressure pump, and washing the chromatographic column body by using
ultrapure water as a mobile phase. The method has the
advantage of simple process; the prepared monolithic column has good pore structure, high
mechanical strength and high column efficiency; during use, the water is taken as the mobile phase, so the waste and
pollution caused by using a large quantity of
organic solvent can be avoided; and in the
separation process, the action force between reagents and the monolithic column can be controlled only by changing temperature, so the separation is simple and feasible, and the separation effect is good.