33 results about "Methylomonas methanolica" patented technology

The invention relates to sesquiterpene dimmers in vernonia anthelmintica, and a preparation method and application. The dimers comprises vernodalin dimer I, vernodalin dimer J and vernodalin dimer K. The preparation method comprises crushing vernonia anthelmintica seeds, percolating and degreasing by petroleum ether, so as to obtain total extractives, then performing gradient elution respectively by using petroleum ether / ethyl acetate, chloroform / methanol and methanol / water, and then performing repeated purification by using semi-preparative high performance liquid chromatograph. Three new sesquiterpene dimers, vernodalin dimer I, J and K are proved to be separated from vernonia anthelmintica seed through analysis on spectrum and mass spectrum data. In-vitro anti-tumor activity research shows that the provided compounds possess relatively obvious cytotoxic activity on human lung cancer cell A-549, human colon cancer cell HCT-15 and human prostate cancer cell PC-3, are applicable to low-toxicity anti-tumor medicines, and are new lead compounds for developing anti-tumor medicines.

A method for producing methane-enriched gas, liquefied petroleum gas and gasoline from synthesis gas. After being heated, the raw material synthesis gas enters a methanol reactor equipped with a methanol synthesis catalyst to react to generate methanol; the outlet material of the methanol reactor enters a methanol reactor equipped with a Catalyst reacts in the methanation reactor to generate methane; the outlet material of the methanation reactor enters the synthetic oil reactor equipped with synthetic oil catalyst to be converted into liquefied petroleum gas and gasoline; the outlet material of the synthetic oil reactor enters the oil-water-gas three-phase separator , the gas phase product obtained by separation is methane-rich gas, and the water phase product is sent to the outside for treatment; the oil phase product enters the rectification tower for rectification separation, and the liquefied petroleum gas product is obtained at the top of the tower, and the gasoline product is obtained at the bottom of the tower. The invention has the advantages of low energy consumption and high efficiency.

The invention specifically relates to an evaporation system and a production process of dimethylformamide. Using carbon monoxide and dimethylamine as raw materials to prepare dimethylformamide under the catalysis of methanol / sodium methoxide has the advantages of good product quality and low cost, but sodium methoxide dissolves in water and is prone to crystallization, and it will catalyze DMF in the evaporation process A reverse reaction occurs to form dimethylamine. The present invention provides a kind of DMF evaporation system and production process applicable to the above-mentioned production mode, and the described production process comprises the following steps: after the crude DMF of the DMF synthesis tower enters the separator for preliminary concentration, it is divided into two routes and the filter is forced Circulation, one way is to add water to the inlet of the second circulation pump to ensure the normal operation of the evaporation circulation pump by adding water, and the other is to inhibit the reverse reaction of DMF. The first circulating pump in the other path plays the role of filtering and discharging the salt mud in the system. The production process can effectively improve the DMF operation cycle and increase the output.

The invention discloses an HPLC method for simultaneous determination of eight active components in Codonopsis pilosula. , Atractylodes Lactone III and 5-Hydroxymethylfurfural, its assay steps are as follows: (1) preparation of test solution; (2) chromatographic condition: C18 chromatographic column, mobile phase consists of methanol-0.01% phosphoric acid aqueous solution, gradient Elution program: 0‑10min, 10% methanol; 10‑25min, 10%‑65% methanol; 25‑40min, 65%‑80% methanol. Wavelength: 0‑5min, 260nm; 5‑6.5min, 210nm; 6.5‑36.5min, 260nm; 36.5‑38min, 220nm; 38‑40min, 260nm. Flow rate 1 mL / min. Column temperature: 30°C. The injection volume was 10 μL. (3) Establishment of standard curve for the determination of the content of eight active ingredients; (4) Determination of the content of active ingredients in the sample. The method is easy to operate, accurate, fast and efficient, can simultaneously measure the contents of eight active components in Codonopsis pilosula medicinal materials, and has great practical value.

The invention discloses a method for separating and purifying Epothilone D based on molecular imprinting. The EpoD molecular imprinting polymer is soaked in a small amount of pure methanol, loaded into a separation column, and methanol-acetic acid, methanol, and methanol-deionized water are sequentially used Rinse the column, vacuum compact the column; dissolve the EpoD crude product in 20-80% methanol aqueous solution, and add it to the installed column; after standing for 20-50min, rinse the column to remove the interference, and further reduce the interference of impurities; Use 10-90% methanol PBS solution as the eluent to elute the column, so that EpoD is completely dissolved in the eluent, collect the eluate and spin evaporate to dryness, and redissolve in pure methanol to obtain a purified EpoD methanol solution. Compared with the existing separation and purification methods, the method is simple, convenient and low in cost, and at the same time, it is specific adsorption in the epothilone D adsorption process, which can achieve better separation effect. It provides possibility for further separation and purification of epothilone D.