An object of the present invention is to provide a
novel protein having the following
amino acid sequence altered for specifically and efficiently binding a
protein to an immobilization carrier via the carboxy terminus. The
protein is used for immobilizing a portion represented by R1-R2 on an immobilization carrier, comprising the
amino acid sequence represented by the general formula R1-R2-R3-R4-R5 [wherein:the sequences are oriented from the
amino terminal side to the carboxy terminal side;the sequence of the R1 portion is the sequence of a subject
protein to be immobilized and contains neither a
lysine residue nor a
cysteine residue;the sequence of the R2 portion may be absent, but when the sequence of the R2 portion is present, the sequence of the R2 portion is a spacer sequence composed of
amino acid residues other than
lysine and
cysteine residues;the sequence of the R3 portion is composed of two residues of amino acid represented by
cysteine-X (where X denotes an
amino acid residue other than
lysine or cysteine);the sequence of the R4 portion may be absent, but when the sequence of the R4 portion is present, the sequence of the R4 portion contains neither a
lysine residue nor a cysteine residue, but contains an acidic
amino acid residue capable of acidifying the
isoelectric point of the entire protein comprising the amino acid sequence represented by the general formula R1-R2-R3-R4-R5; andthe sequence of an R5 portion is an affinity tag sequence for
protein purification.