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Method for preparing superoxide dismutase (SOD)-loaded gamma-poly glutamic acid hydrogel

A polyglutamic acid and hydrogel technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve problems such as SOD inactivation, achieve mild reaction conditions, good biocompatibility, and improve activity The effect of maintaining performance

Inactive Publication Date: 2014-08-13
TIANJIN AROLYN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical modification of the enzyme needs to consider the specificity of the reaction, the toxicity of the reagent, and the reaction conditions; if it is not handled properly, it will cause the inactivation of SOD during the reaction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1. The preparation of the γ-polyglutamic acid hydrogel of SOD, namely SOD-γ-PGA hydrogel

[0014] Taking γ-PGA as the feed ratio basis, γ-PGA: EDC: NHS: SOD: the molar ratio of cystamine dihydrochloride is 1: 0.5: 0.5: 0.0001: 0.1; γ-PGA is dissolved in deionized water, Ultrasonic dissolution; add EDC to ice bath at 4 °C for 15 min, then add NHS and continue to react at 4 °C for 15 min; add SOD to the above activation system, react at room temperature for 3 hours, then stand at 4 °C for 24 hours; then add A certain amount of cystamine dihydrochloride was reacted at room temperature for 3 hours to form a gel; after the reaction, the reaction solution was put into a dialysis bag and dialyzed in deionized water at 4°C for 6 days, changing the water every 12 hours. The obtained dialysate was freeze-dried to obtain SOD-loaded γ-polyglutamic acid hydrogel.

[0015]

Embodiment 2

[0016] Example 2. The preparation of the γ-polyglutamic acid hydrogel loaded with SOD, i.e. the SOD-γ-PGA hydrogel

[0017] Taking γ-PGA as the feed ratio basis, γ-PGA: EDC: NHS: SOD: the molar ratio of cystamine dihydrochloride is 1: 0.75: 0.75: 0.0005: 0.075; γ-PGA is dissolved in deionized water, Ultrasonic dissolution; add EDC to ice bath at 4 °C for 15 min, then add NHS and continue to react at 4 °C for 15 min; add SOD to the above activation system, react at room temperature for 3 hours, then stand at 4 °C for 24 hours; then add A certain amount of cystamine dihydrochloride was reacted at room temperature for 34 hours to form a gel; after the reaction, the reaction solution was put into a dialysis bag and dialyzed in deionized water at 4 °C for 6 days, changing the water every 12 hours. The obtained dialysate was freeze-dried to obtain SOD-loaded γ-polyglutamic acid hydrogel.

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PUM

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Abstract

The invention discloses a method for preparing superoxide dismutase (SOD)-loaded gamma-poly glutamic acid hydrogel. The method comprises the following steps: adopting a 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride / N-hydroxysuccinimide (EDC / NHS) activation system, and forming an SOD-gamma-polyglutamic acid (PGA) immobilized product by using lysine residues and poly glutamic acid side chain carboxyl of SOD; crosslinking the SOD-gamma-PGA obtained by the reaction by using a diamine crosslinking agent cysteamine hydrochloride, so as to form an SOD-gamma-PGA gel system. The gel system can be applied to wound dressing or mask, and can play the antioxidant damage action, and the skin wound is quickly repaired.

Description

[0001] technical field [0002] The invention relates to a method for loading biological enzymes on polymer materials, in particular to a method for loading superoxide dismutase (SOD) on γ-polyglutamic acid (γ-PGA) hydrogel. Background technique [0003] Superoxide dismutase (SOD) is the main antioxidant enzyme for scavenging superoxide free radicals in organisms, and it is widely used in biomedicine, beauty care and other fields. However, direct use of the superoxide dismutase solution has problems such as easy inactivation, short action time, and pulsed dosage. One of the most urgent problems to be solved is to prevent the inactivation of superoxide dismutase, especially in wound repair. The pH of normal skin is neutral to acidic, generally 5-6, and the pH of the wound can even reach 4 during inflammatory reactions. If superoxide dismutase is exposed to this acidic environment for a long time, it will be inactivated and unable to scavenge free radicals. [0004] Loading...

Claims

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Application Information

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IPC IPC(8): A61L15/26A61L15/44A61K8/84A61K8/66A61K8/04A61Q19/08
Inventor 郝政王淑芳苑庆平
Owner TIANJIN AROLYN BIOTECH
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