52 results about "Lactonase" patented technology

The present invention relates to a method comprising the method steps A) Providing at least one compound of the general formula I) where X=divalent organic radical comprising 1 to 19 carbon atoms B) Contacting the compound of the general formula I) with an enzyme E1 selected from the group esterases, lipases and lactonases, characterized in that method step B) is carried out in the presence of at least one aliphatic alcohol comprising 1 to 6 carbon atoms.

The invention relates to the technical field of lactonase, in particular to a lactonase production device which comprises a bin body, a controller is arranged on one side of the bin body, a top cover is arranged on the upper surface of the bin body, a motor is arranged in the center of the top of the top cover, and one side of the top of the top cover communicates with a feeding pipe; a round pipe penetrating through the top cover is arranged at the bottom of the motor, and a round block is arranged at the end, away from the motor, of the round pipe. Meanwhile, high-frequency irregular up-and-down movement of the main fixing blocks and the auxiliary fixing blocks on the outer surfaces of the supporting rods can be achieved through cooperation of the main fixing blocks and the auxiliary fixing blocks on the outer surfaces of the multiple sets of supporting rods, springs and a bottom-layer circular plate, the cleaning effect on the inner wall of the bin body is improved, then high-frequency impact is conducted on the bottom-layer circular plate, and the service life of the bin body is prolonged. And the bottom of the bin body is vibrated and cleaned in cooperation with a large brush, the cleaning effect is guaranteed, and the cleaning performance is improved.

Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolyzed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolyzed lignocellulosic plant biomass.

The invention provides a construction method of a synthetic strain for generating fengycin by transforming xylose through regulating and controlling a Dahms pathway. The construction method comprises the following steps of: connecting a promoter gene P43, xylose dehydrogenase xylB, xylC, a xylosic acid lactonase gene yjhG and a 2-ketone-3-deoxyxylosic acid dehydratase gene yjhH in series to prepare a Dahms xylose pathway module into a shuttle vector pHY300PLK; meanwhile, starting from metabolite glycolaldehyde of the Dahms pathway, connecting an aldehyde dehydrogenase gene aldA, malic acid synthetase aceB, malic acid dehydrogenase mdh and the promoter P43 in series to prepare an glycolaldehyde back-complement TCA regulating and controlling module into a shuttle vector pHP13; co-expressing the two shuttle vectors in a chassis strain to construct a fengycin high-quality strain BSU02 which is synthesized by co-expressing and transforming the xylose through the Dahms module and the glycolaldehyde back-complement TCA regulating and controlling module.

The invention discloses a black mold BFA010-7 with high production of D-pantolactonase obtained through soil screening and low-temperature plasma mutagenesis, the preservation number is CGMCC 14631, and the above-mentioned Aspergillus niger strain BFA010-7 is prepared Application of D‑pantotolactone. In the present invention, the Aspergillus niger mycelium is grown and propagated in the sponge block by embedding, and then the immobilized cell catalyst is prepared by a chemical cross-linking method. The whole process is simple, easy, cheap and practical, and easy to scale up. The prepared immobilized cell catalyst has the advantages of high activity, good operational stability, easy separation and recovery, etc., and has a good application prospect in the industrial production process of calcium D-pantothenate.

The invention discloses recombinant bacteria using xylose to produce glycollic acid and a building method and application of the recombinant bacteria and belongs to the technical field of genetic engineering. The xylose dehydrogenase gene, xylonic acid lactonase gene, xylonic acid dehydratase gene, 3-deoxygenation-D-glycerin ketopentose acid aldolase gene and glycolic aldehyde dehydrogenase gene in the recombinant bacteria using the xylose to produce the glycollic acid are overexpressed. Meanwhile, the invention further provides a preparation method of the recombinant bacteria and a method using the recombinant bacteria to produce the glycollic acid. By the recombinant bacteria, the biological synthesizing path using D-xylose as the carbon source to form the glycollic acid through glycolic aldehyde conversion is achieved for the first time.