Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

N-acyl homoserine lactonase-inorganic hybrid nano-catalyst and preparation thereof

A technology of acyl homoserine lactone and acyl homoserine, which is applied in the field of N-acyl homoserine lactonase-inorganic hybrid nanocatalyst and its preparation, can solve the problems of poor stability and limitation of quenching enzymes, Achieve good recycling rate, improved catalytic activity, and good application prospects

Active Publication Date: 2019-07-09
ZHEJIANG UNIV OF TECH
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of N-acyl homoserine lactonase (AHL-lactonase)-inorganic hybrid nano catalyst, is a kind of AHL-lactonase-inorganic hybrid nano catalyst prepared by self-assembly method , in order to solve the technical problem that the existing group quenching enzymes have poor stability and are limited in practical applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • N-acyl homoserine lactonase-inorganic hybrid nano-catalyst and preparation thereof
  • N-acyl homoserine lactonase-inorganic hybrid nano-catalyst and preparation thereof
  • N-acyl homoserine lactonase-inorganic hybrid nano-catalyst and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Add 5.0 μL of 0.65 μM AHL-lactonase purified enzyme from Salinicolasalaria 1A01339 to 1 mL of 10 mM phosphate buffer with a pH of 8.0, mix well, add 26 μL of 200 mM cobalt chloride, and then place the resulting mixture at 25 °C After standing still for 0.5h, the supernatant was removed by centrifugation, the precipitate was washed 3 times with ultrapure water, and then dried in vacuum to obtain AHL-lactonase-inorganic hybrid nanocatalyst——Co 3 (PO 4 ) 2 @AHL - lactonase.

Embodiment 2

[0037] Add 5.0 μL of 0.65 mM AHL-lactonase purified enzyme from Salinicolasalaria 1A01339 to 1 mL of 10 mM phosphate buffer solution with a pH of 8.0, mix well and add 26 μL of 200 mM manganese sulfate, and then place the resulting mixture at 25° C. After standing for 0.5h, the supernatant was removed, the precipitate was washed 3 times with ultrapure water, and then dried in vacuum to obtain AHL-lactonase-inorganic hybrid nanocatalyst——Mn 3 (PO 4 ) 2 @AHL - lactonase.

Embodiment 3

[0039] Add 5.0 μL 0.65 mM AHL-lactonase purified enzyme from Salinicolasalaria 1A01339 to 1 mL of 10 mM phosphate buffer solution with a pH of 8.0, mix well, add 26 μL 200 mM zinc sulfate, and then place the resulting mixture at 25 ° C After standing for 0.5h, the supernatant was removed, the precipitate was washed 3 times with ultrapure water, and then dried in vacuum to obtain AHL-lactonase-inorganic hybrid nanocatalyst——Zn 3 (PO 4 ) 2 @AHL - lactonase.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an N-acyl homoserine lactonase(AHL-lactonase)-inorganic hybrid nano-catalyst and a preparation method thereof. The nano-catalyst is composed of the organic component and the inorganic component, wherein the organic component is theAHL-lactonase, and the inorganic component is insoluble phosphate, such as zinc, nickel, manganese, cobalt and the like. The preparation method comprises the following steps: adding the AHL-lactonase and soluble metal salt into a phosphate buffer solution respectively, standing the obtained mixed liquid, removing the supernatant, and carryingout vacuum drying to obtain the AHL-lactonase-inorganic hybrid nano-catalyst with small particle size and stable structure. The preparation method is simple and the activity of the obtained AHL-lactonase-inorganic hybrid nano-catalyst is significantly higher than the activityof free enzymes, and the thermal stability is better; the surface area of small-sized complexes is larger, thereby increasing the contact area with substrate in the reaction. The enzyme substrate has a wide spectrum, can effectively degrade many signal molecules, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of enzyme modification, and in particular relates to an N-acyl homoserine lactonase-inorganic hybrid nanometer catalyst and a preparation method thereof. Background technique [0002] Bacteria can sense changes in population density by monitoring the concentration of extracellular signaling molecules. When the signal molecules reach a certain critical density value, they will induce the expression of specific genes and coordinate group behavior. This phenomenon is named quorum sensing (Quorum sensing). Sensing, QS). The pathogenicity of many animal and plant pathogenic bacteria is regulated by the quorum sensing system, and Quorum Quenching (QQ) targeting the bacterial quorum sensing system has been proven to be an effective strategy to control this type of bacterial disease. Population quenching can be achieved by mechanisms that inhibit the synthesis, accumulation, monitoring, or enzymatic degradation or ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96
CPCC12N9/18C12N9/96C12Y301/01081
Inventor 陈艳柳鹏福储消和郭倩韩笑笑竹国津
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com