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Lactonase and method for degrading alpha-zearalanel through lactonase

A technology of zearalenol and lactonase, applied in the field of lactonase, can solve the problem of low degradation activity

Inactive Publication Date: 2017-07-07
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Application Information

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Problems solved by technology

The gene encoding ZHD101 has been successfully expressed in a heterologous host, but product analysis found that due to the low degradation activity of ZHD101 on ZOL, when an insufficient amount of ZHD101 exists, it cannot degrade a small amount of ZOL coexisting in the system, while α- ZOL still has strong estrogen toxicity

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[0019] Some typical embodiments embodying the features and advantages of the present application will be described in detail in the following description. It should be understood that the present application can have various changes in different embodiments without departing from the scope of the present application, and the descriptions and illustrations therein are essentially used for illustration rather than limitation this application.

[0020] In order to increase the industrial application value of zearalenone degrading enzyme, the present application clones the lactone hydrolase ZHD101 gene from Gliocladium roseum, and the expressed protein is a macrocyclic intracellular protein. esterase. After studying the structure of this lactonase, the amino acids involved in the interaction of substrates in its active region were mutated to increase the activity of the enzyme to the substrate derivative α-zearalenol (α-ZOL). The method for modifying lactonase in the present app...

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Abstract

The invention discloses lactonase. An amino acid sequence is the sequence that the 167 amino acid of the serial number 5 is mutated into Histidine from Valine, and the lactonase is used for improving the degrading efficiency of alpha-zearalanel. The invention further discloses a method for degrading the alpha-zearalanel. The alpha-zearalanel is degraded by means of the lactonase, so that the degrading efficiency of the alpha-zearalanel is improved.

Description

technical field [0001] The present application relates to a lactonase, in particular to a lactonase that can improve the degradation efficiency of α-zearalenol. Background technique [0002] Mycotoxins are toxic and harmful secondary metabolites secreted by molds or other fungi. Mold growth and mycotoxin production in grain feed may occur at various stages of grain maturation, transportation, processing and storage. Feed contaminated with mycotoxins can cause animal poisoning, affect immune function, reduce productivity, and even cause serious public health problems. Mycotoxin contamination has become one of the important factors restricting the development of the feed industry and aquaculture. About 25% of crops are contaminated with mycotoxins to varying degrees every year. Taking the United States and Canada as examples, the pollution of mycotoxins makes the livestock industry and the feed industry every year. Lost about $5 billion. The direct economic loss caused by m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/18C12P7/40A23L5/20
CPCC12N9/18C12P7/40C12Y301/01025A23K10/14A23K10/30A23L5/25C12Y301/01A23K20/10
Inventor 郭瑞庭郑迎迎许中霞陈纯琪彭卫刘卫东刘嚞睿马延和
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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