Method for synthesizing antioxidant feruloylated dibutyrated acylglycerol accelerated by solvent-free system enzyme

Abstract

The invention relates to a method for solvent system free enzymic synthesis of anti oxidant, namely, ferulic acid bis-butanoic acid glyceride. The method comprises the following steps: firstly, being catalyzed by thionyl chloride, ferulic acid and absolute ethyl alcohol are heated under reflux and reaction, are dissolved in ethylether, and are extracted by sodium bicarbonate, the ethylether is removed, and then standstill and seed out are performed to obtain ferulic acid ethyl ester which is then purified; secondly, by utilizing the characteristic that glycerol monobutyralte is liquid at room temperature, the obtained ferulic acid ethyl ester and the glycerol monobutyralte are directly used as the reaction medium and the substrate, and are reacted in the catalysis of Novozym435 lipase and purified, thereby obtaining the ferulic acid bis-butanoic acid glyceride. The method has mild and safe reaction condition, and prepared oxidant which maintains the property of the ferulic acid and the butanoic acid is released slowly under the function of lactonase in the human body. The method has the functions of being anti-oxidation and anti-fatigue, and removing harmful free radical in the human body, and also has the functions of preventing and treating various cardiovascular and cerebrovascular diseases. The method does not use organic solvent, thus simple post treatment is ensured and the environmental pollution is reduced.

Description

technical field [0001] The invention belongs to the field of preparation of food antioxidants, in particular to a preparation method of functional food antioxidant ferulic acid dibutyrin in a solvent-free system. Background technique [0002] "Natural, nutritious, multi-functional" has become the current development policy of the food additive industry. The international quality requirements for food additives are: to make food more natural and fresh; to enhance the stability of food quality during storage; to use no or less chemically synthesized additives. The use of biological methods to replace chemical synthesis of safe and efficient new food additives and the development of functional food additives with immune regulation, anti-aging, anti-fatigue, resistance to hypoxia, anti-radiation, regulation of blood lipids, and adjustment of gastrointestinal function are the fields in the world in recent years. direction of development. [0003] Ferulic acid (ferulic acid, FA)...

AI Technical Summary

Problems solved by technology

The solvent-free system enzyme-catalyzed reaction is to simply mix the reactants to carry out the enzyme-catalyzed reaction (the substrate exists in solid or molten form) without organic solvents. It has all the functions of the organic phase enzyme-catalyzed reaction Advantages, such as: the thermodynamic balance of the reaction moves from hydrolysis to synthesis, the enzyme has a high degree of stability, etc.), and at the same time overcomes the shortcomings of organic solvents such as high toxicity, flammability and volatility, pollution to the environment, high recovery and recycling costs, etc.

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