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Erythromycin degrading bacterium RJJ-5 and application thereof

A technology of RJJ-5 and erythromycin, applied in the field of microorganisms, can solve the problems of waste of recyclable components, environmental damage, and high operating cost of antibiotic slag, and achieve the effect of good application value and high erythromycin degradation activity.

Active Publication Date: 2020-06-19
CHANGZHOU UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many shortcomings in these treatment methods, which cannot meet all production needs, environmental requirements, and high commercial costs. For example, the operating cost of incinerating and disposing of antibiotic slag in hazardous waste incinerators is too high, and it has certain damage to the environment, and The inability to effectively utilize the recyclable components results in partial waste, which is not in line with sustainable development; the treatment of antibiotic residues with a huge amount of production and high organic matter content has a huge impact on the environment, so it is not suitable for hazardous waste landfill technology

Method used

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  • Erythromycin degrading bacterium RJJ-5 and application thereof
  • Erythromycin degrading bacterium RJJ-5 and application thereof
  • Erythromycin degrading bacterium RJJ-5 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The screening method of erythromycin-degrading bacteria (Curvularia mebaldsii (Curvularia mebaldsii) RJJ-5) is as follows:

[0022] Weigh a certain amount of bacterial residue, add distilled water, and dilute 10 times. Take 1 mL of the supernatant, add 9 mL of sterile water for gradient dilution, and dilute to 1000 times, take the supernatant and add MSM sterile medium containing erythromycin, and place it in a shaker at 37 ° C and 180 rpm for 5 days. The supernatant obtained above was evenly spread on the MSM solid medium containing erythromycin, then placed in a 37°C incubator and cultured for 3-5 days, and separated on the LB plate by streaking to obtain the erythromycin-degrading bacteria RJJ- 5. The morphology of a single colony is shown in figure 1 shown.

[0023] Strain identification

[0024] The genomic DNA of erythromycin-degrading bacteria RJJ-5 was obtained by shaking and crushing method, and the ITS gene of the strain was amplified by PCR method and sent...

Embodiment 2

[0047] Cultivate the erythromycin-degrading bacteria RJJ-5 in LB liquid medium, collect the bacteria by centrifugation, resuspend with sterile water, and dilute to OD 600 =1, inoculate 1 mL of the selected bacterial strain into 100.0 mL of basal liquid inorganic salt medium containing 100 mg erythromycin, place it in a constant temperature shaker at 180 r / min, cultivate at 37 ° C for 72 hours, and the degradation rate is 71.2%, and the degradation rate of erythromycin The efficiency effect is obvious. ( figure 2 )

Embodiment 3

[0049] The erythromycin-degrading bacteria RJJ-5 was inoculated in 100 grams of sterilized soil according to the inoculum amount of 1%, and 100 mg of erythromycin was added, and cultured at 37°C for 72 hours. The degradation rate was determined to be 51.22%. . ( image 3 )

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Abstract

The invention discloses an erythromycin degrading bacterium RJJ-5 and application thereof, a method for degrading erythromycin and an erythromycin degrading bacterium dry powder. The erythromycin degrading bacterium and the application thereof have the advantages that microorganisms capable of degrading erythromycin are obtained through a microbial technology means according to the characteristicsof erythromycin from specific environmental conditions or through transformation, catalytic degradation is conducted on the erythromycin structure through macrocyclic lactonase, transferase, lyase and the like, and a pollution-free metabolite is obtained. Experiments prove that the erythromycin degrading bacterium RJJ-5 can survive in the environment with erythromycin as the only carbon source, the high erythromycin degrading activity is achieved, and the erythromycin degrading rate can reach 50%-75%. The erythromycin degrading bacterium RJJ-5 can be applied to erythromycin degradation in thepolluted environment and has the good application value in the aspects of water treatment and soil remediation.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an erythromycin-degrading bacterium RJJ-5 and its application. Background technique [0002] my country is a big country in the production and use of antibiotics, and erythromycin is a commonly used antibiotic. A large amount of solid waste is produced in the process of erythromycin, and its main components include bacteria, incompletely utilized medium, degradation products of the medium, secondary metabolites produced by the metabolism of the producing bacteria, and added during the product extraction process. Additives such as inorganic salts, etc. Erythromycin residue contains a lot of nutrients, such as rough eggs, etc., but these nutrients cannot be used directly. The fungal residue waste is identified as hazardous waste by the national department. Improper treatment methods can easily cause environmental pollution and ecological hazards. Therefore, it is necessa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10C02F3/34C12R1/645
CPCC12N1/14B09C1/105C02F3/347C12R2001/645C12N1/145Y02W10/10
Inventor 任建军李春雨李真真郝雨欣张晋呼和涛力
Owner CHANGZHOU UNIV
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