38 results about "Fluorescent derivative" patented technology

The present invention relates to the field of optical recording materials, in particular, fluorescent compounds and matrices suitable for use in optical memory systems, including three dimensional optical memory systems for READ ONLY MEMORY (ROM). In particular, nonfluorescent Rhodamine B lactams are photochemically transformed into fluorescent Rhodamine B derivatives.

Provided herein are methods for identifying a compound that modulates a Ryanodine receptor (Ryr). Fluorescence resonance energy transfer between an FKBP bound to an RyR and fluorescent derivatives of RyR binding partners (e.g., calmodulin) or domain-peptide biosensors is used to provide a readout dependent on the RyR functional state. The methods permit measurement of RyR present in a permeabilized cell or in a purified membrane.

The invention relates to the field of food and agricultural safety detection, in particular to the design and synthesis of a carbazole-based fluorescent derivative reagent with novel structure and its application in the detection of fipronil and fipronil metabolites. Tests have proved that the carbazole fluorescent derivatization reagent designed and synthesized by the present invention has strong fluorescence intensity, and the HPLC of fipronil and its metabolite fipronil sulfoxide can be determined based on the pre-column derivatization established by the derivatization reagent. ‑FLD (High Performance Liquid Chromatography‑Fluorescence Detection) method has high sensitivity, low detection limit and quantification limit, good stability and reproducibility. This method is applied to residual fipronil and its metabolite fipronil sulfoxide in eggs The detection results are accurate and reliable, and are superior to the currently reported HPLC-MS / MS and GC-MS methods. The carbazole fluorescent derivatization reagent designed and synthesized by the present invention has excellent performance, and the pre-column derivatization detection method of fipronil and its metabolites based on it is sensitive, reliable and accurate, and has a good application prospect.

The invention discloses a method for determining the content of glutathione in bacterial cells. The method comprises the following steps: drafting a standard curve of different concentrations of glutathione to the fluorescence intensity; adopting a plate colony-counting method to carry out colony counting; setting a bacterial liquid experiment group for determination and a background fluorescence control group; processing the experiment group and the control group, and detecting by adopting a fluorescence intensity detection method; calculating according to the standard curve to obtain the glutathione content, and calculating according to flat counting to obtain the quantity of sample bacteria; and calculating a ratio of the glutathione content to the quantity of the corresponding bacteria to obtain the content of glutathione in a certain quantity of bacterial cells. An mBBr reagent is adopted in the invention, can go through the cell walls of Gram-positive bacteria, enters the cells, and combines with glutathione to generate a stable fluorescence derivative, and the bacteria are cracked by acetonitrile, so a problem of the wall breaking of the Gram-positive bacteria of the measurement method is solved.

The invention relates to a device and a method for measuring neurotransmitters in the brain dialysate of mammals using enzymatic reactors specific to a certain neurotransmitter, which generate fluorescent derivatives when mixed. The mixture is introduced into an incubation chamber and passes through a temperature controlling system in order to set it to the optimum enzymatic reaction temperature, and the fluorescent derivative generated is passed through a flow fluorescence cell located inside the incubation chamber, where the liquid is impacted with a laser light source via an optical fiber. The fluorescence emitted is captured by a fluorescence detector that sends it to a processing system in order to obtain the corresponding values.

A method of detection separation and identification for expressed trace protein / peptide; and a system therefor. There is provided a method of detecting, separating and identifying a minute amount of expressed protein and / or peptide, characterized in that a fluorescent derivative of protein and / or peptide contained in a test subject sample having been labeled with a fluorescence reagent is applied to HPLC; a fluorescent fraction is collected and subjected to enzymatic hydrolysis; mass-spectrometry of the resultant fluorescence-labeled fragments and non-fluorescence-labeled fragments is carried out; and the thus obtained ion molecular weight information on each of the fragments is collated with an available protein and / or peptide fragment database to thereby accomplish a structural analysis. Further, there is provided an identification system therefor.

The invention relates to the field of novel 23-hydroxybetulinic acid fluorescent derivative organic synthesis and pharmaceutical chemistry and concretely relates to a 23-hydroxybetulinic acid fluorescent probe obtained through connection of a coumarin fluorophore to a 23-hydroxybetulinic acid skeleton. The invention discloses a use of the 23-hydroxybetulinic acid fluorescent derivative in antineoplastic mechanism research and a potential use of the 23-hydroxybetulinic acid fluorescent derivative in cancer treatment and especially relates to a use of the 23-hydroxybetulinic acid fluorescent probe in detection of target cell localization and uptake under action of 23-hydroxybetulinic acid.

The invention discloses a fluoboride fluorescent derivative, and a synthetic method and an application thereof, and belongs to the metal ion detection field. The molecular structure of the fluoborate fluorescent derivative is represented by a formula shown in the specification. The fluoborate fluorescent derivative is used for the fluorescent sensing and analysis of cadmium ions in the environment, allows boron atoms to be exchanged with the cadmium ions after the identification of the cadmium ions, has a good cadmium ion selectivity, has a strong anti-interference capability to other metal ions, can avoid the interferences of other metal ions, especially the interferences of zinc ions, and allows the cadmium ions to be rapidly distinguished from a plurality of metal ions.