38 results about "Fluorescent derivative" patented technology

A method utilising one or more fluorogenic probes, for the detection of nitroreductase activity. The non-fluorescent probes are reduced in the presence of nitroreductase to form fluorescent derivatives that may be detected using fluorescence spectroscopy. In particular, the method may be used to detect and / or identify a plurality of nitroreductase in a single test environment

The mechanism of the UV light-induced reaction between the indole moiety of tryptophan and chloroform, and the structure of the modified tryptophan and polypeptides including such modified tryptophan residues. The excited indole moiety, which is formed upon UV light irradiation, emits a solvated electron which initiates a series of events that yield fluorescent derivatives that have CHO group covalently bound to the indole moiety. These derivatives are herein referred to as formyltryptophan, and are relatively stable. Similar reactions are observed when 5-hydroxytryptophan, 5-fluorotryptophan, or N-methylindolacetate are used in place of tryptophan, or when other haloalkanes, such as trichloracetic acid, trichlorethanol, trichlorethane, bromoform, and iodoactetate are used in place of chloroform. The derivatives can be used in a variety of applications in fluorescence spectroscopy, and for nuclear magnetic resonance, X-ray crystallography, infra-red spectroscopy, circular dicroism and mass spectroscopy. Additionally, the UV light-induced reaction between the indole moiety of tryptophan and haloalkanes can be used to prepare derivatives of tryptophan for chemical cross-linking studies of proteins and peptides.

The mechanism of the UV light-induced reaction between the indole moiety of tryptophan and chloroform, and the structure of the modified tryptophan and polypeptides including such modified tryptophan residues. The excited indole moiety, which is formed upon UV light irradiation, emits a solvated electron which initiates a series of events that yield fluorescent derivatives that have CHO group covalently bound to the indole moiety. These derivatives are herein referred to as formyltryptophan, and are relatively stable. Similar reactions are observed when 5-hydroxytryptophan, 5-fluorotryptophan, or N-methylindolacetate are used in place of tryptophan, or when other haloalkanes, such as trichloracetic acid, trichlorethanol, trichlorethane, bromoform, and iodoactetate are used in place of chloroform. The derivatives can be used in a variety of applications in fluorescence spectroscopy, and for nuclear magnetic resonance, X-ray crystallography, infra-red spectroscopy, circular dicroism and mass spectroscopy. Additionally, the UV light-induced reaction between the indole moiety of tryptophan and haloalkanes can be used to prepare derivatives of tryptophan for chemical cross-linking studies of proteins and peptides.

The present invention relates to the field of optical recording materials, in particular, fluorescent compounds and matrices suitable for use in optical memory systems, including three dimensional optical memory systems for READ ONLY MEMORY (ROM). In particular, nonfluorescent Rhodamine B lactams are photochemically transformed into fluorescent Rhodamine B derivatives.

The invention relates to a method for filtering enzyme applied to catalyze nonaqueous phase system transesterification reaction and belongs to the technical field of enzyme reaction and fluorescence measuration. In the method, a. vinyl ester and primary alcohol substance are catalyzed by enzyme to take transesterification reaction, as one of the reaction products vinyl alcohol generates acetaldehyde by keto-enol tautomerization to ensure that the transesterification reaction is nonreversible; b. the acetaldehyde reacts with 4-diazanyl-7-nitryl-2, 1, 3-benzoxadiazole (NBD-H.NH2NH2) to generate fluorescent derivative, and the fluorescence intensity of the fluorescent derivative is mensurated to reflect the catalytic capability of the enzyme indirectly. The invention has simple operation and can intuitively and easily judge whether enzyme catalysis transesterification reaction in nonaqueous phase can occur or not, the invention can compare the catalytic activity of the enzyme catalyzing the transesterification reaction, and the method can be used for studying on the factor influencing enzymic catalytic reaction.

The invention discloses an antibody-polymer combined body and a fluorescent derivative thereof and a preparation method of the antibody-polymer combined body and the fluorescent derivative. The antibody comprises at least one disulfide bond or free sulfydryl; the polymer is combined to the antibody through connection to the disulfide bond or free sulfydryl of the antibody; according to the method, a specific site of the antibody is modified; a polymer and a fluorescent derivative thereof grow in situ; or a polymer and a fluorescent derivative thereof are prepared in advance; and the antibody-polymer combined body and the fluorescent derivative thereof are prepared in a manner of specific site coupling of the antibody. The method disclosed by the invention is simple, convenient and efficient; the prepared product not only reserves the original biological activity of the antibody, but also improves the water solubility, stability, pharmacokinetics, distribution of organisms and treatment efficacy; the immunogenicity is reduced; in addition, compared with an antibody-fluorescence indicator prepared by the traditional method, the antibody-polymer fluorescent derivative prepared by the method has stronger fluorescence signal and detection signal strength.

A method of detection separation and identification for expressed trace protein / peptide; and a system therefor. There is provided a method of detecting, separating and identifying a minute amount of expressed protein and / or peptide, characterized in that a fluorescent derivative of protein and / or peptide contained in a test subject sample having been labeled with a fluorescence reagent is applied to HPLC; a fluorescent fraction is collected and subjected to enzymatic hydrolysis; mass-spectrometry of the resultant fluorescence-labeled fragments and non-fluorescence-labeled fragments is carried out; and the thus obtained ion molecular weight information on each of the fragments is collated with an available protein and / or peptide fragment database to thereby accomplish a structural analysis. Further, there is provided an identification system therefor.

The invention discloses a fluoboride fluorescent derivative, and a synthetic method and an application thereof, and belongs to the metal ion detection field. The molecular structure of the fluoborate fluorescent derivative is represented by a formula shown in the specification. The fluoborate fluorescent derivative is used for the fluorescent sensing and analysis of cadmium ions in the environment, allows boron atoms to be exchanged with the cadmium ions after the identification of the cadmium ions, has a good cadmium ion selectivity, has a strong anti-interference capability to other metal ions, can avoid the interferences of other metal ions, especially the interferences of zinc ions, and allows the cadmium ions to be rapidly distinguished from a plurality of metal ions.

The present invention relates to the field of optical recording materials, in particular, fluorescent compounds and matrices suitable for use in optical memory systems, including three dimensional optical memory systems for READ ONLY MEMORY (ROM). In particular, nonfluorescent Rhodamine B lactams are photochemically transformed into fluorescent Rhodamine B derivatives.

The invention relates to a novel oridonin fluorescent derivative and concretely relates to an oridonin fluorescent probe prepared by connecting a fluorophore to an oridonin framework. The invention also discloses a preparation method of the oridonin fluorescent derivative and a potential use of the oridonin fluorescent derivative in antitumor mechanism research and cancer treatment and especially relates to a use of the oridonin fluorescent probe in detection of oridonin target cell positioning.

The invention relates to a device and a method for measuring neurotransmitters in the brain dialysate of mammals using enzymatic reactors specific to a certain neurotransmitter, which generate fluorescent derivatives when mixed. The mixture is introduced into an incubation chamber and passes through a temperature controlling system in order to set it to the optimum enzymatic reaction temperature, and the fluorescent derivative generated is passed through a flow fluorescence cell located inside the incubation chamber, where the liquid is impacted with a laser light source via an optical fibre. The fluorescence emitted is captured by a fluorescence detector that sends it to a processing system in order to obtain the corresponding values.

The invention discloses a dual-mode method for rapidly detecting histamine in food, which comprises the steps of performing chemical derivation on histamine and o-phthalaldehyde to form a derivative with fluorescence, performing fluorescence sensitization on beta-cyclodextrin modified nitrogen and zinc doped carbon dots by the histamine fluorescent derivative, adding gold nanoparticles, and performing surface enhanced Raman scattering detection. A Raman scattering signal of the histamine derivative enhanced by beta-CD-N and Zn-CDs is greatly improved, the fluorescence intensity is greatly reduced, and gold nanoparticles have the dual effects of Raman enhancement and fluorescence quenching, so that an SERS and fluorescence dual-mode detection method of histamine is established; the method is applied to detection and analysis of histamine in food, and the result accords with the determination method of biogenic amine in food in GB5009.208-2016 food safety national standard; and the method has the characteristics of high sensitivity, strong specificity, simplicity in operation, rapidness and the like.

The invention discloses an efficient liquid phase chromatographic fluorescence detection method for measuring concentration of perfluorooctanoic acid in a landfill leachate. The method comprises the following steps of (1) carrying out pretreatment on a leachate sample with a certain volume to obtain a solution A; (2) performing constant volume of 10mL of the solution A obtained in the step (1), then adding 1mL and 5mg / mL of derivating agent acetone solution, and next, carrying out a catalytic reaction for 150min in a constant-temperature water bath at the temperature of 70 DEG C to obtain a fluorescent derivative solution; and (3) taking 1 mL of the fluorescent derivative solution, taking Zorbax Eclicpse Plus C18 as a chromatographic column, and taking acetonitrile-water as a flowing phase, performing machine measuring, recording the peak area, obtaining the concentration of perfluorooctanoic acid in the step (2) according to a calculation formula, and next, indirectly calculating theconcentration of perfluorooctanoic acid in the landfill leachate. The method has the advantages of being high in sensitivity, low in detection limit, relatively low in derivatization agent cost, simple in method and the like.

A fluorescent chromophore, its fluorescent derivative and their preparing process are disclosed. The fluorescent chromophore is 8-oxy-8H acenaphtho-(1,2-b) pyrrole-9-nitrile, and is prepared from 2-(2-oxy-2H-acenaphthenyl) propanedinitrile through cyclizing reaction in solvent under the existance of alkali catalyst. The fluorescent derivative is 3,6-bisubstituent-8-oxy-8H -acenaphtho (1,2-b) pyrrole-9-nitrile and is prepared by substitution reaction of the said fluorescent chromophore on neucleophile in solvent. It can be used as ideal fluorescent probe compound with very high fluorescent quantum efficiency (near to 1).

The invention discloses a method for determining the content of glutathione in bacterial cells. The method comprises the following steps: drafting a standard curve of different concentrations of glutathione to the fluorescence intensity; adopting a plate colony-counting method to carry out colony counting; setting a bacterial liquid experiment group for determination and a background fluorescence control group; processing the experiment group and the control group, and detecting by adopting a fluorescence intensity detection method; calculating according to the standard curve to obtain the glutathione content, and calculating according to flat counting to obtain the quantity of sample bacteria; and calculating a ratio of the glutathione content to the quantity of the corresponding bacteria to obtain the content of glutathione in a certain quantity of bacterial cells. An mBBr reagent is adopted in the invention, can go through the cell walls of Gram-positive bacteria, enters the cells, and combines with glutathione to generate a stable fluorescence derivative, and the bacteria are cracked by acetonitrile, so a problem of the wall breaking of the Gram-positive bacteria of the measurement method is solved.

Provided herein are methods for identifying a compound that modulates a Ryanodine receptor (Ryr). Fluorescence resonance energy transfer between an FKBP bound to an RyR and fluorescent derivatives of RyR binding partners (e.g., calmodulin) or domain-peptide biosensors is used to provide a readout dependent on the RyR functional state. The methods permit measurement of RyR present in a permeabilized cell or in a purified membrane.

The invention relates to the field of food and agricultural safety detection, in particular to the design and synthesis of a carbazole-based fluorescent derivative reagent with novel structure and its application in the detection of fipronil and fipronil metabolites. Tests have proved that the carbazole fluorescent derivatization reagent designed and synthesized by the present invention has strong fluorescence intensity, and the HPLC of fipronil and its metabolite fipronil sulfoxide can be determined based on the pre-column derivatization established by the derivatization reagent. ‑FLD (High Performance Liquid Chromatography‑Fluorescence Detection) method has high sensitivity, low detection limit and quantification limit, good stability and reproducibility. This method is applied to residual fipronil and its metabolite fipronil sulfoxide in eggs The detection results are accurate and reliable, and are superior to the currently reported HPLC-MS / MS and GC-MS methods. The carbazole fluorescent derivatization reagent designed and synthesized by the present invention has excellent performance, and the pre-column derivatization detection method of fipronil and its metabolites based on it is sensitive, reliable and accurate, and has a good application prospect.

The invention discloses a method for determining the content of glutathione in bacterial cells. The method comprises the following steps: drafting a standard curve of different concentrations of glutathione to the fluorescence intensity; adopting a plate colony-counting method to carry out colony counting; setting a bacterial liquid experiment group for determination and a background fluorescence control group; processing the experiment group and the control group, and detecting by adopting a fluorescence intensity detection method; calculating according to the standard curve to obtain the glutathione content, and calculating according to flat counting to obtain the quantity of sample bacteria; and calculating a ratio of the glutathione content to the quantity of the corresponding bacteria to obtain the content of glutathione in a certain quantity of bacterial cells. An mBBr reagent is adopted in the invention, can go through the cell walls of Gram-positive bacteria, enters the cells, and combines with glutathione to generate a stable fluorescence derivative, and the bacteria are cracked by acetonitrile, so a problem of the wall breaking of the Gram-positive bacteria of the measurement method is solved.

The invention relates to a device and a method for measuring neurotransmitters in the brain dialysate of mammals using enzymatic reactors specific to a certain neurotransmitter, which generate fluorescent derivatives when mixed. The mixture is introduced into an incubation chamber and passes through a temperature controlling system in order to set it to the optimum enzymatic reaction temperature, and the fluorescent derivative generated is passed through a flow fluorescence cell located inside the incubation chamber, where the liquid is impacted with a laser light source via an optical fiber. The fluorescence emitted is captured by a fluorescence detector that sends it to a processing system in order to obtain the corresponding values.

Antibody-macromolecular conjugates, fluorescent derivatives thereof and their preparation methods. The antibody contains at least one disulfide bond or free sulfhydryl group, and the macromolecule is bound to the antibody through the disulfide bond or free sulfhydryl group attached to the antibody. The method is to modify the specific site of the antibody and grow the polymer and its fluorescent derivatives in situ; or prepare the polymer and its fluorescent derivatives in advance, and then couple with the specific site of the antibody to prepare the antibody-polymer Conjugates and their fluorescent derivatives. The method of the present invention is simple and efficient, and the prepared product not only retains the original biological activity of the antibody but also improves the water solubility, stability, pharmacokinetics, biodistribution and therapeutic efficacy of the antibody, and reduces its immunogenicity. In addition, the present invention Compared with antibody-fluorescent markers prepared by traditional methods, the prepared antibody-polymer fluorescent derivatives have stronger fluorescent signals and detection signal intensity.

A method of detection separation and identification for expressed trace protein / peptide; and a system therefor. There is provided a method of detecting, separating and identifying a minute amount of expressed protein and / or peptide, characterized in that a fluorescent derivative of protein and / or peptide contained in a test subject sample having been labeled with a fluorescence reagent is applied to HPLC; a fluorescent fraction is collected and subjected to enzymatic hydrolysis; mass-spectrometry of the resultant fluorescence-labeled fragments and non-fluorescence-labeled fragments is carried out; and the thus obtained ion molecular weight information on each of the fragments is collated with an available protein and / or peptide fragment database to thereby accomplish a structural analysis. Further, there is provided an identification system therefor.

The invention provides 3,5-dibenzimidazolyl-8-thiophenyl fluoroboron fluorescent derivatives, belonging to the technical field of fluoroboron fluorescent derivatives. The 3,5-dibenzimidazolyl-8-thiophenyl fluoroboron fluorescent derivatives have a structure shown in a formula (I), and the formula (I) is described in the description. The invention also provides a preparation method of the fluoroboron fluorescent derivatives. A synthetic route used in the method is a C-H / N-H direct oxidative coupling reaction; compared with the traditional fluoroboron fluorescent ammoniation preparation technology, the method provided by the invention reduces the organic synthesis steps, avoids the cumbersome process of pre-activation of a substrate, improves the reaction compatibility and increases the total yield of the synthetic reaction. After the fluoroboron fluorescent derivatives provided by the invention are applied to the endoplasmic reticulum specific fluorescence development and fluorescent labeling of cells, the cost of the derivatives is far lower than that of the commercially available cell endoplasmic reticulum labeling reagents, and the problems that in the prior art, an endoplasmic reticulum target agent is complicated in structure, inconvenient to synthesize and expensive in price can be solved.

The invention relates to a method and system of polysaccharide, quantitative test, which is that polysaccharide is hydrolysis after isolation through chromatographic column then reacts with fluorescence biochemical reagent, and water content of polysaccharide is acquired by fluorescence detector measuring fluorescence derivative created. The invention has increased sensitivity of detection greatly by making polysaccharide react with fluorescence biochemical reagent 6 after its hydrolysis, is suitable for quantitative test of water content of polysaccharide to various kinds, whose testing limitation can reach tens of ng.

The invention discloses a method for rapid fluorescence detection of chromium content. The method comprises the following steps: histamine, tyramine and fluorescamine are adopted to form a fluorescent derivative in a phosphoric acid buffer solution with pH of 8, the derivative generates strong fluorescence emission at 488nm under the excitation wavelength of 390nm, the characteristic that nitrogen and hydroxyl of the derivative have excellent affinity to Cr (VI) is utilized to generate specific complexation, Cr (VI) induces quenching of a fluorescence signal, and a chromium fluorescence probe is constructed and can be used for fluorescence detection of Cr (VI) in a sample; under the alkaline condition, Cr (III) is oxidized into Cr (VI) by hydrogen peroxide, the total chromium in the sample can be measured. The method can be used for morphological analysis of chromium, the detection limit of the method is 0.05 mg / kg, the method is high in specificity, and other metal ions do not have the phenomenon; The method is applied to detection of chromium in environmental food, a result accords with a related national standard determination method; the method has the characteristics of simple operation, high sensitivity, rapidness and the like.

The invention relates to the field of food and agricultural safety detection, in particular to the design and synthesis of a coumarin-based fluorescent derivative reagent with novel structure and its application in the detection of fipronil. Tests have proved that the coumarin-based fluorescent derivative reagent designed and synthesized by the present invention has strong fluorescence intensity, and the HPLC-FLD method for determining fipronil based on the pre-column derivatization established by the derivative reagent has high sensitivity and low detection limit. and low limit of quantification, good stability and reproducibility, the method is applied to the detection of fipronil residues in eggs, the results are accurate and reliable, and are superior to the currently reported HPLC-MS / MS and GC-MS methods. The coumarin-based fluorescent derivatization reagent designed and synthesized by the present invention has excellent performance, and the pre-column derivatization detection method of fipronil based on it is sensitive, reliable and accurate, and has a good application prospect.

The invention discloses a fluorescence detection method for a phthalate plasticizer in an oil substance. The fluorescence detection method comprises the following steps of (1) preparing a phthalate standard curve, (2) preparing saponification hydrolysate, (3) preparing a fluorescent derivative, and (4) performing detection and conversion. According to the method, NaOH is adopted to completely saponify the oil substance, phthalate is hydrolyzed into sodium phthalate, then petroleum ether is added to thoroughly separate grease from an aqueous solution dissolved with the sodium phthalate under centrifugal action, the sodium phthalate is neutralized into phthalic acid under the action of hydrogen peroxide, a peroxy root (-O-) structure with an electron donating effect is combined to carboxyl of the phthalic acid, so that the phthalate which originally exists in the grease and cannot generate fluorescence is separated out and fluorescence is generated, and a fluorescence detection mode becomes possible. The method has the advantages of simple operation, less reagents, mild and easily controllable detection conditions, low cost and wide linear range of a working curve.

The invention discloses a method for quick determination of sulfonamide residues in food. The method includes the technological steps of a, weighing the weight of the food with the sulfonamide residues accurately, adding the weighed food with the sulfonamide residues into distilled water, mixing the food evenly prior to centrifugation, and taking out liquid supernatant; b, preparing a sulfonamide fluorescent derivative to obtain a standard substance enrichment phase; c, preparing a sample sulfonamide fluorescent derivative to obtain a sample enrichment phase; d, spotting the standard substance enrichment phase obtained in the step b and the sample enrichment phase obtained in the step c on a silica gel plate, observing fluorescence intensity of the standard substance enrichment phase and the sample enrichment phase under an ultraviolet lamp with ultraviolet light wavelength of 365 nm, and judging the sulfonamide residues in the food through comparison between the fluorescence intensity of the standard substance enrichment phase and that of the sample enrichment phase. The method is capable of detecting the sulfonamide residues in the food rapidly and effectively according to the above technological step design.

The invention relates to a fluorescent probe for detecting cytosine and a preparation method of the fluorescent probe. The fluorescent probe is a high fluorescence derivative containing three dimethylpyrrole BODIPY primitives. The fluorescent probe is of a structure making the three dimethyl pyrrole BODIPY primitives connected to the same alkyl carbon, and the chemical name is tri-([4-(acetoxyphenyl]-(4,4-difluoro-1,3,5,7-tetramethyl-4-boron-3a,4a-bipyrrole))oxygen methylene propane. The tri BODIPY shows bright yellow in the solution state, very strong fluorescence-emission is achieved at 510nm, the tri BODIPY fluorescence quantum yield reaches 0.97, fluorescence is obviously enhanced after complexation is conducted with cytosine, fluorescence enhancement and cytosine concentration are in linear relation, the minimum detection limit reaches 8.2 micrometer, interference of other biomolecules is small, and the fluorescent probe is an ideal rapid cytosine detection sensing probe.

The invention discloses a fluoboride fluorescent derivative, and a synthetic method and an application thereof, and belongs to the metal ion detection field. The molecular structure of the fluoborate fluorescent derivative is represented by a formula shown in the specification. The fluoborate fluorescent derivative is used for the fluorescent sensing and analysis of cadmium ions in the environment, allows boron atoms to be exchanged with the cadmium ions after the identification of the cadmium ions, has a good cadmium ion selectivity, has a strong anti-interference capability to other metal ions, can avoid the interferences of other metal ions, especially the interferences of zinc ions, and allows the cadmium ions to be rapidly distinguished from a plurality of metal ions.