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Dual-mode method for rapidly detecting histamine in food

A dual-mode, histamine technology, applied in the field of chemical analysis and detection, achieves high sensitivity, sensitive fluorescence-SERS dual-mode detection, and simple operation

Active Publication Date: 2021-06-29
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Gold nanometers play a dual role. One is as a Raman enhancer to enhance the Raman spectrum signal, and at the same time it is a fluorescence quencher, which quenches the fluorescence of carbon dots and histamine fluorescent derivatives to improve the Raman detection signal. , there is no report related to the present invention

Method used

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  • Dual-mode method for rapidly detecting histamine in food
  • Dual-mode method for rapidly detecting histamine in food
  • Dual-mode method for rapidly detecting histamine in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: Determination of histamine in cheese sample

[0038] (1) Preparation of nitrogen and zinc doped carbon dots (N,Zn-CDs): Weigh 1g of citric acid and 0.3g of zinc acetate, dissolve in 30mL of ultrapure water, mix well, add 2.5mL of ethylenediamine, Sonicate for 15 minutes, transfer to a polytetrafluoroethylene reaction tank, microwave digestion at 850W, 150°C for 30 minutes, cool naturally, filter with a filter membrane with a pore size of 0.22 μm, and then use a dialysis bag with a molecular weight cut-off of 3000Da for dialysis treatment for 24 hours , to obtain water-soluble N,Zn-CDs;

[0039] (2) Preparation of β-cyclodextrin-modified nitrogen and zinc-doped carbon dots (β-CD-N,Zn-CDs): Add pH 4 acetic acid-sodium acetate buffer to 8 mL of N,Zn-CDs prepared above Solution 7mL, mix well, add 0.5g 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide hydrochloride (EDC·HCl) and 0.5g N-hydroxysulfosuccinimide Amine (NHS), react at room temperature for 40min; t...

Embodiment 2

[0055] Embodiment 2: the mensuration of histamine in the squid sample

[0056] (1) Preparation of nitrogen and zinc doped carbon dots (N,Zn-CDs): Weigh 1g of citric acid and 0.4g of zinc acetate, dissolve in 40mL of ultrapure water, mix well, add 3.0mL of ethylenediamine, Ultrasound for 20 minutes, and transfer to a polytetrafluoroethylene reaction tank, microwave digestion at 850W, 150°C for 35 minutes, after natural cooling, filter with a filter membrane with a pore size of 0.22 μm, and then perform dialysis treatment with a dialysis bag with a molecular weight cut-off of 3000Da for 24 hours , to obtain water-soluble N,Zn-CDs;

[0057](2) Preparation of β-cyclodextrin-modified nitrogen and zinc-doped carbon dots (β-CD-N,Zn-CDs): Add pH 4 acetic acid-sodium acetate buffer to 10 mL of N,Zn-CDs prepared above Solution 9mL, mix well, add 0.5g 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide hydrochloride (EDC·HCl) and 0.5g N-hydroxysulfosuccinimide Amine (NHS), react at room t...

Embodiment 3

[0065] Embodiment 3: Determination of sausage sample histamine content

[0066] (1) Preparation of nitrogen and zinc doped carbon dots (N,Zn-CDs): same as Example 1;

[0067] (2) Preparation of nitrogen and zinc doped carbon dots (β-CD-N, Zn-CDs) modified by β-cyclodextrin: same as Example 1;

[0068] (3) The preparation of the gold nano solution is the same as in Example 1;

[0069] (4) The drawing of the molecular fluorescence detection working curve of histamine is the same as in Example 1;

[0070] (5) The SERS detection work curve drawing of histamine, the wave number determination of histamine SERS spectrum detection and the SERS analysis of the standard substance are the same as in Example 1;

[0071] (6) The preparation of Carrez solution I and Carrez II solution is the same as in Example 1;

[0072] (7) Pretreatment of sausage samples: same as in Example 2;

[0073] (8) Determination of molecular fluorescence of sausage samples: same as in Example 1, the content o...

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Abstract

The invention discloses a dual-mode method for rapidly detecting histamine in food, which comprises the steps of performing chemical derivation on histamine and o-phthalaldehyde to form a derivative with fluorescence, performing fluorescence sensitization on beta-cyclodextrin modified nitrogen and zinc doped carbon dots by the histamine fluorescent derivative, adding gold nanoparticles, and performing surface enhanced Raman scattering detection. A Raman scattering signal of the histamine derivative enhanced by beta-CD-N and Zn-CDs is greatly improved, the fluorescence intensity is greatly reduced, and gold nanoparticles have the dual effects of Raman enhancement and fluorescence quenching, so that an SERS and fluorescence dual-mode detection method of histamine is established; the method is applied to detection and analysis of histamine in food, and the result accords with the determination method of biogenic amine in food in GB5009.208-2016 food safety national standard; and the method has the characteristics of high sensitivity, strong specificity, simplicity in operation, rapidness and the like.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a dual-mode rapid detection method for histamine in food. Background technique [0002] Before the food is fermented or when the food is spoiled, the bacteria will produce "histamine" biological amine compounds during the metabolic process. When the human body takes too much of this substance, it may cause headaches or other symptoms for those who are intolerant to histamine. Thereby causing a certain impact on health. Therefore, it is meaningful to establish a rapid, sensitive and simple method for detecting histamine content in food. At present, the determination methods of histamine in food include biological method, thin layer chromatography, liquid chromatography, fluorescence method, spectrophotometry and so on. [0003] Surface-enhanced Raman scattering (SERS) spectroscopy can achieve qualitative and quantitative detection and interface stress ana...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨德志李克相李秋兰杨亚玲
Owner KUNMING UNIV OF SCI & TECH
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