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Method for quick determination of sulfonamide residues in food

A detection method and residue technology, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve problems such as human health hazards, and achieve rapid and effective detection

Inactive Publication Date: 2017-04-26
蔡璇
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Problems solved by technology

Extensive use of sulfonamides results in residues in most animal-source foods, and human health will be harmed after people eat animal-source foods with excessive sulfonamide residues

Method used

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Embodiment Construction

[0015] The present invention will be described below in conjunction with specific embodiments.

[0016] A rapid detection method for sulfonamide residues in food, comprising the following process steps, specifically:

[0017] a. Accurately weigh the weight of the sulfa residue food, and add the weighed sulfa residue food into distilled water, the amount of distilled water is 3-6ml / g, mix and centrifuge, take out the supernatant, and set aside;

[0018] b. Preparation of sulfonamide fluorescent derivatives: prepare a standard aqueous solution with a sulfonamide concentration of 0.001-50 mg / l, and add fluorescamine methanol solution with a mass volume percentage of 0.05-0.1% to the above standard aqueous solution, and let it stand for 30-40 minutes at room temperature. minutes, then remove the upper aqueous phase by centrifugal phase separation to obtain the enriched phase of the standard;

[0019] c. Preparation of sample sulfonamide fluorescent derivatives: take the supernata...

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Abstract

The invention discloses a method for quick determination of sulfonamide residues in food. The method includes the technological steps of a, weighing the weight of the food with the sulfonamide residues accurately, adding the weighed food with the sulfonamide residues into distilled water, mixing the food evenly prior to centrifugation, and taking out liquid supernatant; b, preparing a sulfonamide fluorescent derivative to obtain a standard substance enrichment phase; c, preparing a sample sulfonamide fluorescent derivative to obtain a sample enrichment phase; d, spotting the standard substance enrichment phase obtained in the step b and the sample enrichment phase obtained in the step c on a silica gel plate, observing fluorescence intensity of the standard substance enrichment phase and the sample enrichment phase under an ultraviolet lamp with ultraviolet light wavelength of 365 nm, and judging the sulfonamide residues in the food through comparison between the fluorescence intensity of the standard substance enrichment phase and that of the sample enrichment phase. The method is capable of detecting the sulfonamide residues in the food rapidly and effectively according to the above technological step design.

Description

technical field [0001] The invention relates to the technical field of food detection methods, in particular to a rapid detection method for sulfonamide residues in food. Background technique [0002] Animal source foods such as milk and internal products generally contain rich nutrients such as protein, fat, amino acid, sugar, calcium, phosphorus, and iron. The proportion is also increasing. [0003] Among them, sulfonamides are widely used in animal husbandry production for the prevention and treatment of livestock and poultry diseases due to their advantages of low cost, high curative effect, and short course of treatment. Extensive use of sulfonamides results in residues in most animal-source foods, and people eating animal-source foods with excessive sulfonamide residues will cause harm to human health. [0004] Therefore, it is very necessary to detect the sulfa residues in food. Contents of the invention [0005] The object of the present invention is to provide ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 蔡璇
Owner 蔡璇
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